FlyBase Allele Report: Dmel\Cdc50[sgRNA.U6]

General Information

Symbol

Dmel\Cdc50^sgRNA.U6

Species

D. melanogaster

Name

FlyBase ID

FBal0343394

Feature type

allele

Associated gene

Dmel\Cdc50

Associated Insertion(s)

Carried in Construct

P{U6-Cdc50.sgRNA}

Also Known As

gRNA-CDC50

Key Links

Transgenic product class

sgRNA

(Sapar et al., 2018)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Sapar et al., 2018)

Mutagen

in vitro construct

(Sapar et al., 2018)

Progenitor genotype

Carried in construct

P{U6-Cdc50.sgRNA}

Cytology

Description

CRISPR construct designed to knockout the CDC50 gene when combined with a source of Cas9 endonuclease. Constitutively expresses two sgRNAs, both of which target coding exon sequence that is shared by all CDC50 splice isoforms.

(Sapar et al., 2018)

Allele components

Component

Use(s)

Encoded product / tool

Cdc50

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval multidendritic class IV neuron | larval stage, with Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

Detailed Description

Statement

Reference

The knockout of CDC50 in class IV dendritic arborizing neurons by the combination of CDC50^sgRNA.U6 and Spyo\Cas9^ppk.PS leads to some dendrite debris being detected on the underlying epidermal cells, but does not affect dendrite density or dendrite dendrite field size.

(Sapar et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2}, Cdc50^sgRNA.U6, Scer\GAL4^DcG, Spyo\Cas9^ppk.PS has abnormal size | larval stage phenotype, suppressible by drpr^indel/drpr^indel

(Sapar et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^ppk.PH, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8 is a suppressor of abnormal stress response | larval stage phenotype of Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Scer\GAL4^ppk.PH, Spyo\Cas9^ppk.PP, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Scer\GAL4^ppk.PH, Spyo\Cas9^ppk.PP, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6 is a suppressor of abnormal stress response | larval stage phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Spyo\Cas9^ppk.PP/Cdc50^sgRNA.U6 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Spyo\Cas9^ppk.PP/Cdc50^sgRNA.U6 is a suppressor | partially of abnormal stress response | larval stage phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Scer\GAL4^ppk.PH, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Other

Statement

Reference

Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2}, Cdc50^sgRNA.U6, Scer\GAL4^DcG, Spyo\Cas9^ppk.PS has abnormal size | larval stage phenotype

(Sapar et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, enhanceable by Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG}/Ecol\lexA^ppk.PU

(Sapar et al., 2018)

NOT Enhanced by

Statement

Reference

Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, non-enhanceable by ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

Suppressed by

Statement

Reference

Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, suppressible by drpr^indel/drpr^indel

(Sapar et al., 2018)

Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2}, Cdc50^sgRNA.U6, Scer\GAL4^DcG, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, suppressible by drpr^indel/drpr^indel

(Sapar et al., 2018)

Cdc50^sgRNA.U6, Ecol\lexA^ppk.PU, Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG}, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, suppressible by drpr^indel/drpr^indel

(Sapar et al., 2018)

Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, suppressible | partially by Scer\GAL4^ppk.PH/Mmus\Xkr8^UAS.Tag:FLAG

(Sapar et al., 2018)

NOT suppressed by

Statement

Reference

Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype, non-suppressible by ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

Enhancer of

Statement

Reference

Spyo\Cas9^ppk.PS/Cdc50^sgRNA.U6 is an enhancer of larval multidendritic class IV neuron | larval stage phenotype of Ecol\lexA^ppk.PU, Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG}

(Sapar et al., 2018)

Spyo\Cas9^ppk.PS/Cdc50^sgRNA.U6 is an enhancer of dendrite | larval stage phenotype of Ecol\lexA^ppk.PU, Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG}

(Sapar et al., 2018)

Spyo\Cas9^ppk.PS/Cdc50^sgRNA.U6 is an enhancer of dendritic tree | larval stage phenotype of Ecol\lexA^ppk.PU, Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG}

(Sapar et al., 2018)

NOT Enhancer of

Statement

Reference

Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS is a non-enhancer of larval multidendritic class IV neuron | larval stage phenotype of ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

Spyo\Cas9^ppk.PS/Cdc50^sgRNA.U6 is a non-enhancer of larval multidendritic class IV neuron | larval stage phenotype of Ecol\lexA^ppk.PU, Mmus\Xkr8^{lexAop.Tag:FLAG}

(Sapar et al., 2018)

Spyo\Cas9^ppk.PS/Cdc50^sgRNA.U6 is a non-enhancer of dendrite | larval stage phenotype of Ecol\lexA^ppk.PU, Mmus\Xkr8^{lexAop.Tag:FLAG}

(Sapar et al., 2018)

Spyo\Cas9^ppk.PS/Cdc50^sgRNA.U6 is a non-enhancer of dendritic tree | larval stage phenotype of Ecol\lexA^ppk.PU, Mmus\Xkr8^{lexAop.Tag:FLAG}

(Sapar et al., 2018)

Suppressor of

Statement

Reference

Scer\GAL4^ppk.PH, Spyo\Cas9^ppk.PP, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6 is a suppressor of dendrite | larval stage phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Scer\GAL4^ppk.PH, Spyo\Cas9^ppk.PP, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6 is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Spyo\Cas9^ppk.PP/Cdc50^sgRNA.U6 is a suppressor | partially of dendrite | larval stage phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Spyo\Cas9^ppk.PP/Cdc50^sgRNA.U6 is a suppressor | partially of larval dorsal multidendritic neuron ddaC phenotype of Mmus\Wld^S.UAS, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Scer\GAL4^ppk.PH, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8 is a suppressor of dendrite | larval stage phenotype of Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

Scer\GAL4^ppk.PH, Mmus\Ano6^{D430G-L.UAS.Tag:FLAG}, Cdc50^sgRNA.U6, Spyo\Cas9^SOP.scE1x8 is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Sarm^U6:3.gRNA, Spyo\Cas9^SOP.scE1x8

(Ji et al., 2022)

NOT Suppressor of

Statement

Reference

Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS is a non-suppressor of larval multidendritic class IV neuron | larval stage phenotype of ATP8A^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

Other

Statement

Reference

Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2}, Cdc50^sgRNA.U6, Scer\GAL4^DcG, Spyo\Cas9^ppk.PS has larval multidendritic class IV neuron | larval stage phenotype

(Sapar et al., 2018)

Cdc50^sgRNA.U6, Ecol\lexA^ppk.PU, Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG} has larval multidendritic class IV neuron | larval stage phenotype

(Sapar et al., 2018)

Cdc50^sgRNA.U6, Ecol\lexA^ppk.PU, Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG} has vesicle | larval stage phenotype

(Sapar et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The combined tissue-specific knockout of CDC50 (CDC50^sgRNA.U6+Spyo\Cas9^ppk.PS) and expression of Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG} under the control of Ecol\lexA^ppk.PU leads to higher levels of dendrite debris from class IV dendritic arborizing neurons being detected on the underlying epidermal cells than upon either CDC50 knockout or Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG} expression; this phenotype is more severe upon heat shock. This "double mutant" background leads to significant vesicle shedding by class IV dendritic arborizing neurons compared to controls, which is suppressed by drpr^indel homozygosity.

The combined tissue-specific knockout of CDC50 (CDC50^sgRNA.U6+Spyo\Cas9^ppk.PS) and expression of Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2} under the control of Scer\GAL4^DcG leads to short dendrites in class IV dendritic arborizing neurons, as compared to CDC50 knockout alone; this phenotype is suppressed by drpr^indel homozygosity.

The tissue-specific double knockout of CDC50 and ATP8A (CDC50^sgRNA.U6+ATP8A^sgRNA.U6+Spyo\Cas9^ppk.PS) in class IV dendritic arborizing neurons does not affect dendrite density or dendrite dendrite field size, as compared to controls.

(Sapar et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CDC50^sgRNA.U6

Cdc50^sgRNA.U6

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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