FB2024_03 , released June 25, 2024
Recombinant Construct: P{U6-Cdc50.sgRNA}
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General Information
Symbol
P{U6-Cdc50.sgRNA}
FlyBase ID
FBtp0130925
Feature type
transgenic_transposable_element
Component Allele(s)
Cdc50sgRNA.U6
Also Known As
gRNA-CDC50
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Cdc50sgRNA.U6
Transgenic product class
sgRNA
(Sapar et al., 2018)
Component
Use(s)
Encoded product / tool
Cdc50
Description

CRISPR construct designed to knockout the CDC50 gene when combined with a source of Cas9 endonuclease. Constitutively expresses two sgRNAs, both of which target coding exon sequence that is shared by all CDC50 splice isoforms.

(Sapar et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
CDC50 KO
(Sapar et al., 2018)
P{U6-CDC50.sgRNA}
(FlyBase, 1992-)
P{U6-Cdc50.sgRNA}
(FlyBase, 1996-)
gRNA-CDC50
(Ji et al., 2023, Ji et al., 2022, Sapar et al., 2018)
Secondary FlyBase IDs
    References (5)