A TI{CRIMIC.TG4.1} DNA cassette has been inserted into sr, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.1} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: GATTAGTTGCGGACTCCATGCGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.TG4.1} cassette is predicted to be accompanied by a deletion of 9bp of genomic sequence flanking the insertion site.
lethal (with Df(3R)BSC510)
srCR00452-TG4.1/Df(3R)BSC510 is not rescued by Scer\GAL4sr-CR00452-TG4.1/srb.UAS