FlyBase Allele Report: Dmel\sr[b.UAS]

General Information

Symbol

Dmel\sr^b.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0065677

Feature type

allele

Associated gene

Dmel\sr

Associated Insertion(s)

Carried in Construct

P{UAS-sr.b.V}

Also Known As

UAS-sr

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Vorbruggen and Jackle, 1997)

Progenitor genotype

Carried in construct

P{UAS-sr.b.V}

Cytology

Description

An EcoRI/XbaI fragment of the sr b cDNA (nucleotides 405 to 4458) is expressed under the control of UAS regulatory sequences.

(Vorbruggen and Jackle, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^sca-C253

(Usui et al., 2004)

visible, with Scer\GAL4^sr-md710

(Usui et al., 2004)

Phenotype Manifest In

abdominal 5 lateral transverse muscle cell | embryonic stage, with Scer\GAL4^hry.6

(Vorbruggen and Jackle, 1997)

abdominal 5 ventral longitudinal muscle cell | embryonic stage, with Scer\GAL4^hry.6

(Vorbruggen and Jackle, 1997)

abdominal 6 lateral transverse muscle cell | embryonic stage, with Scer\GAL4^hry.6

(Vorbruggen and Jackle, 1997)

abdominal 6 ventral longitudinal muscle cell | embryonic stage, with Scer\GAL4^hry.6

(Vorbruggen and Jackle, 1997)

abdominal 7 lateral transverse muscle cell | embryonic stage, with Scer\GAL4^hry.6

(Vorbruggen and Jackle, 1997)

abdominal 7 ventral longitudinal muscle cell | embryonic stage, with Scer\GAL4^hry.6

(Vorbruggen and Jackle, 1997)

embryonic/larval somatic muscle cell, with Scer\GAL4^en-e16E

(Vorbruggen and Jackle, 1997)

embryonic/larval somatic muscle cell, with Scer\GAL4^hs.2sev

(Vorbruggen and Jackle, 1997)

embryonic/larval somatic muscle cell, with Scer\GAL4^ptc-559.1

(Vorbruggen and Jackle, 1997)

embryonic/larval somatic muscle cell | ventral, with Scer\GAL4^sim.PS

(Vorbruggen and Jackle, 1997)

lateral transverse muscle cell | embryonic stage, with Scer\GAL4^en-e16E

(Vorbruggen and Jackle, 1997)

mesothoracic tergum & chaeta, with Scer\GAL4^sca-C253

(Usui et al., 2004)

mesothoracic tergum & microchaeta, with Scer\GAL4^sr-md710

(Usui et al., 2004)

Detailed Description

Statement

Reference

Expression of sr^b.Scer\UAS under the control of Scer\GAL4^sr-md710 results in a severe decrease in the number of microchaetae on the notum. Expression of sr^b.Scer\UAS under the control of Scer\GAL4^sca-C253 results in a total lack of bristles on the notum.

(Usui et al., 2004)

Expression of sr^b.Scer\UAS in the developing mesoderm using Scer\GAL4^how-24B does not cause muscle defects. Expression of sr^b.Scer\UAS in the ventral midline using Scer\GAL4^sim.PS results in strong distortion of the ventral muscles, which frequently cross the midline. Expression of sr^b.Scer\UAS using Scer\GAL4^en-e16E produces a disturbed muscle pattern, and lateral transverse muscles with abnormally elongated processes are seen. Expression of sr^b.Scer\UAS using Scer\GAL4^ptc-559.1 produces a disturbed muscle pattern, and fused myotubes of abnormal shape are seen. Expression of sr^b.Scer\UAS using Scer\GAL4^h.6 results in the absence of ventral longitudinal and lateral transversal muscles in abdominal segments 5 to 7. Ubiquitous expression of sr^b.Scer\UAS using Scer\GAL4^hs.2sev results in an abnormal muscle pattern only when expression occurs prior to embryonic stage 15. When expressed at stage 12 using Scer\GAL4^hs.2sev, abnormal outgrowth of muscles is seen.

(Vorbruggen and Jackle, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

sr^b.UAS/Scer\GAL4^sca-C253 is a suppressor of visible phenotype of Scer\GAL4^sca-C253, sc^UAS.cHa

(Usui et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

sr^b.UAS/Scer\GAL4^sca-C253 is a suppressor of mesothoracic tergum & macrochaeta | ectopic phenotype of Scer\GAL4^sca-C253, sc^UAS.cHa

(Usui et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of sr^b.Scer\UAS removes most of the ectopic bristles on the notum caused by expression of sc^Scer\UAS.cHa under the control of Scer\GAL4^sca-C253 and also removes many of the extant ones.

(Usui et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^{sr-CR00452-TG4.1}/sr^b.UAS fails to rescue sr^{CR00452-TG4.1}/Df(3R)BSC510

(Lee et al., 2018)

Comments

Images (0)

Mutant

Wild-type