FlyBase Allele Report: Hsap\RELA[AD.VT050660]

General Information

Symbol

Hsap\RELA^AD.VT050660

Species

H. sapiens

Name

FlyBase ID

FBal0340364

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{VT050660-p65.AD}

Also Known As

VT050660-p65ADZp, VT50660-p65ADZp

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Bidaye et al., 2014)

Progenitor genotype

Carried in construct

P{VT050660-p65.AD}

Cytology

Description

The VT050660 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Bidaye et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

VT050660

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

Comment: when combined with P{VT008453-GAL4.DBD}

when combined with P{VT019059-GAL4.DBD}

when combined with P{VT037220-GAL4.DBD}

when combined with P{VT049484-GAL4.DBD}

(Bidaye et al., 2014)

adult moonwalker descending neuron

Comment: when combined with P{VT008453-GAL4.DBD}

when combined with P{VT044845-GAL4.DBD} (combination referred to as 'MDN-1-GAL4')

when combined with P{VT049484-lexA.DBD} (combination referred to as 'MDN+MAN-LexA')

when combined with P{VT014014-GAL4.DBD}

when combined with P{VT044845-GAL4.DBD} (combination referred to as 'MDN3-GAL4')

when combined with P{VT049484-lexA.DBD}

when combined with P{VT019059-GAL4.DBD}

when combined with P{VT037220-GAL4.DBD}

when combined with P{VT044845-GAL4.DBD}

when combined with P{VT049484-GAL4.DBD}

(Israel et al., 2022, Sen et al., 2019, Bidaye et al., 2014)

adult ventral nerve cord | restricted

Comment: when combined with P{VT019059-GAL4.DBD}

when combined with P{VT037220-GAL4.DBD}

(Bidaye et al., 2014)

adult moonwalker ascending neuron

Comment: when combined with P{VT037220-GAL4.DBD}

when combined with P{VT049484-lexA.DBD}

when combined with P{VT049484-lexA.DBD} (combination referred to as 'MDN+MAN-LexA')

when combined with P{VT049484-GAL4.DBD}

(Israel et al., 2022, Sen et al., 2019, Bidaye et al., 2014)

peripheral nervous system | restricted

Comment: when combined with P{VT044845-GAL4.DBD}

(Bidaye et al., 2014)

oviposition descending neuron oviDNa | subset

Comment: when combined with P{VT028160-GAL4.DBD} (combination referred to as 'oviDN-SS1')

(Wang et al., 2020)

oviposition descending neuron oviDNb

Comment: when combined with P{VT028160-GAL4.DBD} (combination referred to as 'oviDN-SS1')

(Wang et al., 2020)

oviposition descending neuron oviDN

Comment: when combined with P{VT028160-GAL4.DBD} (combination referred to as 'SS46540')

(Wang et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.VT037220

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.VT044845

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.VT049484

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.VT008453, TrpA1^UAS.cvPa

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.VT014014, TrpA1^UAS.cvPa

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.VT019059, TrpA1^UAS.cvPa

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.VT037220, TrpA1^UAS.cvPa

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.VT044845, TrpA1^UAS.cvPa

(Bidaye et al., 2014)

abnormal locomotor behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.VT049484, TrpA1^UAS.cvPa

(Bidaye et al., 2014)

Phenotype Manifest In

oocyte | increased number, with CNMaR^KK106827, Scer\GAL4^DBD.VT028160

(Grmai et al., 2024)

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.cvPa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT049484 and Hsap\RELA^AD.VT050660, or the split-GAL4 pair of Scer\GAL4^DBD.VT037220 and Hsap\RELA^AD.VT050660, or the split-GAL4 pair of Scer\GAL4^DBD.VT044845 and Hsap\RELA^AD.VT050660, results in a significant increase in the total distance and bout distance of backwards walking, with a significant decrease in forwards walking, compared to controls.

Thermogenetic activation (using TrpA1^UAS.cvPa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT014014 and Hsap\RELA^AD.VT050660, or the split-GAL4 pair of Scer\GAL4^DBD.VT019059 and Hsap\RELA^AD.VT050660, but not the split-GAL4 pair of Scer\GAL4^DBD.VT008453 and Hsap\RELA^AD.VT050660, results in a small, significant increase in the total distance, but not the bout distance, of backwards walking, compared to controls; all three of these combinations significantly decrease forwards walking, compared to controls.

Inhibition (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT049484 and Hsap\RELA^AD.VT050660, or the split-GAL4 pair of Scer\GAL4^DBD.VT037220 and Hsap\RELA^AD.VT050660, or the split-GAL4 pair of Scer\GAL4^DBD.VT044845 and Hsap\RELA^AD.VT050660, but not the split-GAL4 pair of Scer\GAL4^DBD.VT014014 and Hsap\RELA^AD.VT050660, nor the split-GAL4 pair of Scer\GAL4^DBD.VT008453 and Hsap\RELA^AD.VT050660, nor the split-GAL4 pair of Scer\GAL4^DBD.VT019059 and Hsap\RELA^AD.VT050660, significantly reduces backing-up behavior when adult flies reach a narrow dead end, compared to controls; in the case of Scer\GAL4^DBD.VT037220 and Hsap\RELA^AD.VT050660 only, there is a small, significant increase in total forwards walking distance in unimpeded conditions, compared to controls.