The VT049484 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).
Comment: when combined with P{VT050660-p65.AD}
Comment: when combined with P{VT050660-p65.AD}
Thermogenetic activation (using TrpA1UAS.cvPa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT049484 and Hsap\RELAAD.VT050660 results in a significant increase in the total distance and bout distance of backwards walking, with a significant decrease in forwards walking, compared to controls.
Inhibition (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT049484 and Hsap\RELAAD.VT050660 significantly reduces backing-up behavior when adult flies reach a narrow dead end, compared to controls; there is no significant effect on walking direction in unimpeded conditions, compared to controls.