FlyBase Allele Report: Dmel\ena[UAS.Tag:polyHis]

General Information

Symbol

Dmel\ena^{UAS.Tag:polyHis}

Species

D. melanogaster

Name

FlyBase ID

FBal0340223

Feature type

allele

Associated gene

Dmel\ena

Associated Insertion(s)

Carried in Construct

P{UAS-ena.His6}

Also Known As

UAS-ena, UAS-ena.His6

Key Links

Transgenic product class

cDNA

(Comer et al., 1998)

wild_type

(Comer et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Comer et al., 1998)

wild_type

(Comer et al., 1998)

Mutagen

in vitro construct

(Merdes, 2010.2.24, Comer et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-ena.His6}

Cytology

Description

UAS sequences drive expression of ena tagged with Tag:polyHis.

(Merdes, 2010.2.24)

UASt regulatory sequences drive expression of a wild-type ena cDNA. The protein is tagged with Tag:polyHis.

(Comer et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^how-24B

(Loya et al., 2009)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^how-24B

(Loya et al., 2014)

abnormal size, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

Phenotype Manifest In

embryonic/larval neuromuscular junction, with Scer\GAL4^how-24B

(Loya et al., 2009)

follicle cell & actin filament, with Scer\GAL4^CY2

(Baum and Perrimon, 2001)

NMJ bouton, with Scer\GAL4^how-24B

(Loya et al., 2009)

NMJ bouton | third instar larval stage, with Scer\GAL4^how-24B

(Loya et al., 2014)

nurse cell & actin filament, with Scer\GAL4^{VP16.mat.αTub67C}

(Baum and Perrimon, 2001)

subsynaptic reticulum | third instar larval stage, with Scer\GAL4^how-24B

(Loya et al., 2014)

wing, with Scer\GAL4^Bx-MS1096

(Lyulcheva et al., 2008)

Detailed Description

Statement

Reference

Third instar larvae expressing ena^{Scer\UAS.T:Zzzz\His6} under the control of Scer\GAL4^RapGAP1-OK6 show normal number of satellite boutons on neuromuscular junctions relative to controls.

(Sakuma et al., 2016)

Third instar larvae expressing ena^Scer\UAS.cCa under the control of Scer\GAL4^how-24B show a significant decrease in the number of synaptic boutons and in presynaptic arbor expansion at the neuromuscular junction compared to controls. There is a significant reduction in subsynaptic reticulum thickness, area and fold complexity compared to controls, while presynaptic bouton area is normal.

Larvae expressing ena^Scer\UAS.cCa under the control of Scer\GAL4^how-24B show no significant changes in evoked excitatory junctional potential (EJP) amplitude, miniature EJP (mEJP) amplitude, mEJP frequency and quantal content at the neuromuscular junction compared to controls.

(Loya et al., 2014)

Expression of ena^Scer\UAS.cCa under the control of Scer\GAL4^how-24B results in a reduction in the size and complexity of the muscle 6/7 neuromuscular junction. A reduced number of boutons is seen, along with reduced expansion of the presynaptic terminal arbor and fewer axonal branches. The neuromuscular junctions of larvae expressing ena^Scer\UAS.cCa under the pan-neural Scer\GAL4^elav.PLu driver are indistinguishable from controls.

(Loya et al., 2009)

Over expression of ena^Scer\UAS.cCa in the wing using Scer\GAL4^Bx-MS1096 results in tissue overgrowth.

(Lyulcheva et al., 2008)

Larvae expressing ena^Scer\UAS.cCa under the control of Scer\GAL4^elav-C155, Scer\GAL4^hs.PB or Scer\GAL4^Act5C.PI do not show axonal transport phenotypes.

(Martin et al., 2005)

Expression of ena^Scer\UAS.cCa under the control of Scer\GAL4^CY2 or Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} induces the formation of punctate cytoplasmic F-actin structures associated with clumps of ena protein.

(Baum and Perrimon, 2001)

ISNb bypass phenotypes are not produced when ena^Scer\UAS.cCa is expressed under the control of Scer\GAL4^elav.PLu.

(Wills et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^elav.PLu/ena^{UAS.Tag:polyHis} is a suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, ena^{UAS.Tag:polyHis} has wing phenotype, non-enhanceable by pico^{UAS.Tag:polyHis,Tag:MYC}

(Lyulcheva et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, ena^{UAS.Tag:polyHis} has wing phenotype, suppressible by pico^RNAi.UAS

(Lyulcheva et al., 2008)

Suppressor of

Statement

Reference

Scer\GAL4^elav.PLu/ena^{UAS.Tag:polyHis} is a suppressor of larval longitudinal connective | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Scer\GAL4^elav.PLu/ena^{UAS.Tag:polyHis} is a suppressor of commissure | embryonic stage 16 phenotype of robo1¹, sli²

(Keleman et al., 2005)

Scer\GAL4^Pan-R7/ena^{UAS.Tag:polyHis} is a suppressor | partially of photoreceptor cell R7 & axon phenotype of Lar^C12/Lar²¹²⁷

(Maurel-Zaffran et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Tissue overgrowth resulting from expression of ena^Scer\UAS.cCa in the wing using Scer\GAL4^Bx-MS1096 is not enhanced by co-expression of pico^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}.

Tissue overgrowth resulting from expression of ena^Scer\UAS.cCa in the wing using Scer\GAL4^Bx-MS1096 is suppressed by co-expressing a copy of pico^IR.Scer\UAS.

(Lyulcheva et al., 2008)

Expression of ena^Scer\UAS.cCa under the control of Scer\GAL4^elav.PLu suppresses the midline crossing phenotype seen in transheterozygous sli² robo¹ stage 16 embryos.

(Keleman et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^neu/ena^{UAS.Tag:polyHis} partially rescues ena^GC5/ena^GC1

(Wills et al., 1999)

Scer\GAL4^e22c/ena^{UAS.Tag:polyHis} partially rescues ena^GC5/ena^GC1

(Comer et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29959

w¹¹¹⁸; P{UAS-ena.His6}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

ena^{Scer\UAS.T:Zzzz\His6.cMa}

ena^{Scer\UAS.T:Zzzz\His6}

ena^Scer\UAS.cCa

ena^{UAS.Tag:polyHis}

enb^Scer\UAS.cCa

enb^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Comer

(Comer et al., 1998)

Secondary FlyBase IDs

FBal0241978
FBal0086641

References (14)

Report Sections

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