FlyBase Allele Report: Dmel\spz5[37]

General Information

Symbol

Dmel\spz5³⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0338012

Feature type

allele

Associated gene

Dmel\spz5

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ulian-Benitez et al., 2017)

Mutagen

FLPase

(Ulian-Benitez et al., 2017)

Progenitor genotype

PBac{RB}spz5^e03444

(Ulian-Benitez et al., 2017)

PBac{WH}Shab^f05893

(Ulian-Benitez et al., 2017)

Cytology

Description

27kb deletion that removes the translation start site and first exon of spz5. Part of the neighbouring Shab gene is also deleted.

(Ulian-Benitez et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

abnormal neuroanatomy | larval stage (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

abnormal size | larval stage (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

viable (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

Phenotype Manifest In

axon | larval stage (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

neuromuscular junction | larval stage (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

presynaptic active zone | larval stage (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

type I bouton | larval stage (with Df(3L)Exel6092)

(Ulian-Benitez et al., 2017)

Detailed Description

Statement

Reference

spz5³⁷/Df(3L)Exel6092 transheterozygous larvae are more frequently still and crawl at significantly lower average speeds (despite being able to achieve the same fastest speeds), as compared to controls. Their larval NMJs exhibit several morphological defects compared to controls, including significant decreases in the number of type Ib boutons, in the axonal length and in the number of NMJ branch points, and a significant increase in the number of active zones.

(Ulian-Benitez et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^Toll-7

(Ulian-Benitez et al., 2017)

NOT Enhanced by

Statement

Reference

Df(3L)Exel6092/spz5³⁷ has abnormal locomotor behavior | larval stage phenotype, non-enhanceable by kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has abnormal size | larval stage phenotype, non-enhanceable by kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Suppressed by

Statement

Reference

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by kek6^{UAS.mRFP(Unk)}/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has abnormal size | larval stage phenotype, suppressible | partially by kek6^{UAS.mRFP(Unk)}/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by kek6^{UAS.mRFP(Unk)}/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has abnormal neuroanatomy | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has abnormal size | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has abnormal neuroanatomy | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has abnormal size | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has abnormal neuroanatomy | larval stage phenotype, suppressible by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has abnormal neuroanatomy | larval stage phenotype, suppressible by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

Enhancer of

Statement

Reference

Df(3L)Exel6092/spz5³⁷ is an enhancer of abnormal neuroanatomy | larval stage phenotype of kek6³⁴/Df(3L)Exel6092

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ is an enhancer of abnormal size | larval stage phenotype of kek6³⁴/Df(3L)Exel6092

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ is an enhancer of abnormal locomotor behavior | larval stage phenotype of kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Other

Statement

Reference

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has viable phenotype

(Ulian-Benitez et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has type I bouton | larval stage phenotype, enhanceable by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^Toll-7

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has neuromuscular junction | larval stage phenotype, enhanceable by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^Toll-7

(Ulian-Benitez et al., 2017)

NOT Enhanced by

Statement

Reference

Df(3L)Exel6092/spz5³⁷ has neuromuscular junction | larval stage phenotype, non-enhanceable by kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has type I bouton | larval stage phenotype, non-enhanceable by kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has presynaptic active zone | larval stage phenotype, non-enhanceable by kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has axon | larval stage phenotype, non-enhanceable by kek6³⁴/Df(3R)ED6361

(Ulian-Benitez et al., 2017)

Suppressed by

Statement

Reference

Df(3L)Exel6092/spz5³⁷ has axon | larval stage phenotype, suppressible | partially by kek6^{UAS.mRFP(Unk)}/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has neuromuscular junction | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has axon | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has neuromuscular junction | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has axon | larval stage phenotype, suppressible by CaMKII^T287D.UAS/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has neuromuscular junction | larval stage phenotype, suppressible by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has type I bouton | larval stage phenotype, suppressible by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has neuromuscular junction | larval stage phenotype, suppressible by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷, kek6³⁴/Df(3R)ED6361 has type I bouton | larval stage phenotype, suppressible by Vap33^{UAS.Tag:FLAG,Tag:HA}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ has type I bouton | larval stage phenotype, suppressible | partially by kek6^{UAS.mRFP(Unk)}/Scer\GAL4^Toll-6-D42

(Ulian-Benitez et al., 2017)

Enhancer of

Statement

Reference

Df(3L)Exel6092/spz5³⁷ is an enhancer of neuromuscular junction | larval stage phenotype of kek6³⁴/Df(3L)Exel6092

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ is an enhancer of type I bouton | larval stage phenotype of kek6³⁴/Df(3L)Exel6092

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ is an enhancer of presynaptic active zone | larval stage phenotype of kek6³⁴/Df(3L)Exel6092

(Ulian-Benitez et al., 2017)

Df(3L)Exel6092/spz5³⁷ is an enhancer of axon | larval stage phenotype of kek6³⁴/Df(3L)Exel6092

(Ulian-Benitez et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

spz5³⁷/Df(3L)Exel6092, kek6³⁴/Df(3R)ED6361 double transheterozygotes are viable and larvae exhibit a impaired locomotion phenotype (more frequent immobility and slower average speed) which is similar to that observed in spz5³⁷/Df(3L)Exel6092 transheterozygotes (although travelling shorter distances) and more severe than that observed in kek6³⁴/Df(3R)ED6361 transheterozygotes. These double mutants show larval NMJ defects that are similar to those of spz5³⁷/Df(3L)Exel6092 and more severe than those of kek6³⁴/Df(3R)ED6361.

The decrease in type I bouton number observed at the larval neuromuscular junction of spz5³⁷/Df(3L)Exel6092 single transheterozygotes and of spz5³⁷/Df(3L)Exel6092, kek6³⁴/Df(3R)ED6361 double transheterozygotes is suppressed by either the expression of kek6^{UAS.mRFP(Unk)} under the control of Scer\GAL4^Toll-6-D42 or by expression of Vap33^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^elav.PU; the decrease observed in the double mutants is enhanced by the expression of Vap33^{Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^Toll-7. The decrease in axon length observed at the larval neuromuscular junction of these single and double transheterozygotes is also suppressed by the expression of kek6^{UAS.mRFP(Unk)} under the control of Scer\GAL4^Toll-6-D42.

The decreased axonal length observed at the larval neuromuscular junction of either spz5³⁷/Df(3L)Exel6092 single transheterozygotes or spz5³⁷/Df(3L)Exel6092, kek6³⁴/Df(3R)ED6361 double transheterozygotes is suppressed by the expression of CaMKII^{T287D.Scer\UAS} under the control of Scer\GAL4^Toll-6-D42.

(Ulian-Benitez et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

DNT2³⁷

(Ulian-Benitez et al., 2017)

spz5³⁷

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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