FB2024_03 , released June 25, 2024
Allele: Dmel\Vap33UAS.Tag:FLAG,Tag:HA
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General Information
Symbol
Dmel\Vap33UAS.Tag:FLAG,Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0269830
Feature type
allele
Associated gene
Dmel\Vap33
Associated Insertion(s)
Carried in Construct
P{UAS-FLAG.Vap-33-1.HA}
Key Links
Transgenic product class
wild_type
(Tsuda et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Tsuda et al., 2008)
Mutagen
in vitro construct
(Tsuda et al., 2008)
Progenitor genotype
Carried in construct
P{UAS-FLAG.Vap-33-1.HA}
Cytology
Description

UASt regulatory sequences drive expression of Vap33 tagged at the N-terminal end with 3x Tag:FLAG and at the C-terminal end with 2x Tag:HA.

(Tsuda et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Vap33
Tagged with
Tag:FLAG
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Vap33Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4Toll-6-D42 does not lead to significant changes the number of larval neuromuscular junction type I boutons, as compared to controls.

      (Ulian-Benitez et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Suppressor of
      Statement
      Reference

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4unspecified is a suppressor of axon | somatic clone | adult stage phenotype of αCOP2750

      (Maddison et al., 2023)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4unspecified is a suppressor of mitochondrion | somatic clone | adult stage | decreased number phenotype of αCOP2750

      (Maddison et al., 2023)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4unspecified is a suppressor of combined L1-L3 nerve | somatic clone | adult stage phenotype of αCOP2750

      (Maddison et al., 2023)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4unspecified is a suppressor | partially of endoplasmic reticulum | somatic clone | adult stage | decreased number phenotype of αCOP2750

      (Maddison et al., 2023)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4elav.PU is a suppressor of neuromuscular junction | larval stage phenotype of Df(3L)Exel6092/spz537

      (Ulian-Benitez et al., 2017)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4elav.PU is a suppressor of type I bouton | larval stage phenotype of Df(3L)Exel6092/spz537

      (Ulian-Benitez et al., 2017)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4elav.PU is a suppressor of neuromuscular junction | larval stage phenotype of Df(3L)Exel6092/spz537, kek634/Df(3R)ED6361

      (Ulian-Benitez et al., 2017)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4elav.PU is a suppressor of type I bouton | larval stage phenotype of Df(3L)Exel6092/spz537, kek634/Df(3R)ED6361

      (Ulian-Benitez et al., 2017)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4Toll-6-D42 is a suppressor of neuromuscular junction | larval stage phenotype of kek634/Df(3L)Exel6092

      (Ulian-Benitez et al., 2017)

      Vap33UAS.Tag:FLAG,Tag:HA/Scer\GAL4Toll-6-D42 is a suppressor of type I bouton | larval stage phenotype of kek634/Df(3L)Exel6092

      (Ulian-Benitez et al., 2017)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      spz537/Df(3L)Exel6092, kek634/Df(3R)ED6361 double transheterozygotes are viable and larvae exhibit a impaired locomotion phenotype (more frequent immobility and slower average speed) which is similar to that observed in spz537/Df(3L)Exel6092 transheterozygotes (although travelling shorter distances) and more severe than that observed in kek634/Df(3R)ED6361 transheterozygotes. These double mutants do not have more severe larval NMJ defects than either single mutant.

      The expression of Vap33Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4Toll-6-D42 suppresses the decrease in type I bouton number observed at the larval neuromuscular junction of kek634/Df(3R)ED6361 single transheterozygotes; expression under the control of Scer\GAL4elav.PU suppresses the decrease in type I bouton number observed at the larval neuromuscular junction of spz537/Df(3L)Exel6092 single transheterozygotes and of spz537/Df(3L)Exel6092, kek634/Df(3R)ED6361 double transheterozygotes; expression under the control of Scer\GAL4Toll-7 enhances the decrease in type Ib boutons observed in the double mutants.

      (Ulian-Benitez et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Vap-33-1Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1
      Vap-33AScer\UAS.T:Zzzz\FLAG,T:Ivir\HA1
      Vap33Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1
      Vap33UAS.Tag:FLAG,Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (5)