FlyBase Allele Report: Dmel\Psn[RNAi.UAS.2]

General Information

Symbol

Dmel\Psn^RNAi.UAS.2

Species

D. melanogaster

Name

FlyBase ID

FBal0327447

Feature type

allele

Associated gene

Dmel\Psn

Associated Insertion(s)

Carried in Construct

P{UAS-Psn.RNAi.2}

Key Links

Transgenic product class

RNAi_reagent

(Kang et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kang et al., 2017)

Mutagen

in vitro construct

(Kang et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-Psn.RNAi.2}

Cytology

Description

UAS regulatory sequences drive expression of 21bp shRNA targeting the 7th exon of Psn.

(Kang et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Psn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease

CEA

(Kang et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Nct^RNAi.UAS.2

(Kang et al., 2017)

model of Alzheimer's disease

is exacerbated by Nct^RNAi.UAS.2

(Kang et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain, with Scer\GAL4^elav-C155

(Kang et al., 2017)

adult brain | progressive, with Scer\GAL4^elav-C155

(Kang et al., 2017)

eye | adult stage | female limited, with Scer\GAL4^elav-C155

(Kang et al., 2017)

wing | adult stage, with Scer\GAL4^bbg-C96

(Kang et al., 2017)

wing | adult stage | female limited, with Scer\GAL4^elav-C155

(Kang et al., 2017)

wing margin | adult stage, with Scer\GAL4^bbg-C96

(Kang et al., 2017)

wing vein | adult stage, with Scer\GAL4^bbg-C96

(Kang et al., 2017)

Detailed Description

Statement

Reference

The expression of Psn^{dsRNA.shRNA.Scer\UAS.2} under the control of Scer\GAL4^Act5C.PU leads to early- to mid-pupal lethality, as compared to control. Expression under the control of Scer\GAL4^bbg-C96 leads to wing defects, ranging from severe wing vein and blistering phenotypes to wing margin loss, as compared to controls. Constitutive expression of Psn^{dsRNA.shRNA.Scer\UAS.2} under the control of Scer\GAL4^elav-C155 leads to pupal lethality in males, semi-lethality in females, and the surviving adult females show a severely decreased lifespan, severe locomotor defects in negative geotaxis assays, wrinkled wings in a third of the population and a rough eye phenotype, as compared to controls; adulthood-only expression (temporally-controlled by Gal80[ts]) leads to no obvious defects in gender ratio or in the eye, but leads to a significant decrease in adult lifespan, age-dependent decrease in adult locomotion (significant decreases in negative geotaxis assay score at day 50, but not at days 10 and 30 post eclosion), age-dependent increase in the number of vacuoli in the adult brain (significant and progressive increase from day 30 until day 50 post eclosion) and to a significant increase in the number of apoptotic cells in 30 days post eclosion brains, as compared to controls.

(Kang et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 has short lived phenotype, enhanceable by Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

NOT Enhanced by

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 has increased cell death | adult stage phenotype, non-enhanceable by Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

NOT suppressed by

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 has lethal - all die during pupal stage | male limited phenotype, non-suppressible by Hsap\PSEN1^UAS.VTR, Scer\GAL4^elav-C155

(Kang et al., 2017)

Enhancer of

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 is an enhancer of short lived phenotype of Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

NOT Enhancer of

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 is a non-enhancer of increased cell death | adult stage phenotype of Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 has adult brain | progressive phenotype, enhanceable by Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

NOT Enhanced by

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 has adult brain phenotype, non-enhanceable by Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

NOT suppressed by

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 has wing | female limited | adult stage phenotype, non-suppressible by Hsap\PSEN1^UAS.VTR, Scer\GAL4^elav-C155

(Kang et al., 2017)

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 has eye | female limited | adult stage phenotype, non-suppressible by Hsap\PSEN1^UAS.VTR, Scer\GAL4^elav-C155

(Kang et al., 2017)

Enhancer of

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 is an enhancer of adult brain | progressive phenotype of Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

NOT Enhancer of

Statement

Reference

Psn^RNAi.UAS.2, Scer\GAL4^elav-C155 is a non-enhancer of adult brain phenotype of Nct^RNAi.UAS.2, Scer\GAL4^elav-C155

(Kang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The adulthood-only co-expression of Psn^{dsRNA.shRNA.Scer\UAS.2} and Nct^{dsRNA.shRNA.Scer\UAS.2} under the combined control of Scer\GAL4^elav-C155 and Gal80[ts] enhances the decrease in adult lifespan and the increase in the number of vacuoli, but not of apoptotic cells, in the adult brain, as compared to the adulthood-only expression of Psn^{dsRNA.shRNA.Scer\UAS.2} alone or Nct^{dsRNA.shRNA.Scer\UAS.2} alone.

(Kang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Both the pupal lethality in males and the rough eye and wrinkled wing phenotypes in females observed upon the expression of Psn^{dsRNA.shRNA.Scer\UAS.2} under the control of Scer\GAL4^elav-C155 are not suppressed by the co-expression of Hsap\PSEN1^Scer\UAS.VTR.

(Kang et al., 2017)

Complementation and Rescue Data

Partially rescued by

Psn^RNAi.UAS.2 is partially rescued by Scer\GAL4^elav-C155/Psn^+14.UAS

(Kang et al., 2017)

Comments

The pupal lethality in males and the semi-lethality in females observed upon the expression of Psn^{dsRNA.shRNA.Scer\UAS.2} under the control of Scer\GAL4^elav-C155 are partially suppressed by the co-expression of Psn^+14.Scer\UAS; while the rough eye and wrinkled wing phenotypes in adults females are also partially rescued by the Psn^+14.Scer\UAS expression, the rescued adult males now present these phenotypes, as compared to controls.

(Kang et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Psn^RNAi.UAS.2

Psn^{dsRNA.shRNA.Scer\UAS.2}

Psn^{dsRNA.shRNA.UAS.2}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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