The expression of NctdsRNA.shRNA.Scer\UAS.2 under the control of Scer\GAL4Act5C.PU leads to early-pupal lethality, as compared to control. Expression under the control of Scer\GAL4bbg-C96 leads to wing defects, ranging from severe wing vein and blistering phenotypes to wing margin loss, as compared to controls. Constitutive expression of NctdsRNA.shRNA.Scer\UAS.2 under the control of Scer\GAL4elav-C155 leads to pupal lethality in males, semi-lethality in females, and the surviving adult females show a severely decreased lifespan, severe locomotor defects in negative geotaxis assays and rough eye and wrinkled wing phenotypes, as compared to controls; adulthood-only expression (temporally-controlled by Gal80[ts]) leads to no obvious defects in gender ratio or in the eye, but leads to a significant decrease in adult lifespan, age-dependent decrease in adult locomotion (significant decreases in negative geotaxis assay score at day 50, but not at days 10 and 30 post eclosion), age-dependent increase in the number of vacuoli in the adult brain (significant and progressive increase from day 30 until day 50 post eclosion) and to a significant increase in the number of apoptotic cells in 30 days post eclosion brains, as compared to controls.
NctRNAi.UAS.2, Scer\GAL4elav-C155 has short lived phenotype, enhanceable by PsnRNAi.UAS.2, Scer\GAL4elav-C155
NctRNAi.UAS.2, Scer\GAL4elav-C155 has increased cell death | adult stage phenotype, non-enhanceable by PsnRNAi.UAS.2, Scer\GAL4elav-C155
NctRNAi.UAS.2, Scer\GAL4elav-C155 is an enhancer of short lived phenotype of PsnRNAi.UAS.2, Scer\GAL4elav-C155
NctRNAi.UAS.2, Scer\GAL4elav-C155 is a non-enhancer of increased cell death | adult stage phenotype of PsnRNAi.UAS.2, Scer\GAL4elav-C155
NctRNAi.UAS.2, Scer\GAL4elav-C155 has adult brain | progressive phenotype, enhanceable by PsnRNAi.UAS.2, Scer\GAL4elav-C155
NctRNAi.UAS.2, Scer\GAL4elav-C155 has adult brain phenotype, non-enhanceable by PsnRNAi.UAS.2, Scer\GAL4elav-C155
NctRNAi.UAS.2, Scer\GAL4elav-C155 is an enhancer of adult brain | progressive phenotype of PsnRNAi.UAS.2, Scer\GAL4elav-C155
NctRNAi.UAS.2, Scer\GAL4elav-C155 is a non-enhancer of adult brain phenotype of PsnRNAi.UAS.2, Scer\GAL4elav-C155
The adulthood-only co-expression of NctdsRNA.shRNA.Scer\UAS.2 and PsndsRNA.shRNA.Scer\UAS.2 under the combined control of Scer\GAL4elav-C155 and Gal80[ts] enhances the decrease in adult lifespan and the increase in the number of vacuoli, but not of apoptotic cells, in the adult brain, as compared to the adulthood-only expression of NctdsRNA.shRNA.Scer\UAS.2 alone or PsndsRNA.shRNA.Scer\UAS.2 alone.