UASp regulatory sequences drive expression of the Cele\unc-104 motor domain (amino acids 1-389) along with a leucine zipper for dimerization, fused to the C-terminus of Khc (amino acids 905-975, includes the microtubule binding site). The construct is tagged with mTagBFP2 at the C-terminal.
Cele\unc-104::KhcLZ.UASp.mTagBFP2/Scer\GAL4elav.PLu is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of KhcKO.mutA
Cele\unc-104::KhcLZ.UASp.mTagBFP2, Scer\GAL4VP16.nanos.UTR is a suppressor of oocyte phenotype of KhcGL00330, Scer\GAL4VP16.nanos.UTR
Cele\unc-104::KhcLZ.UASp.mTagBFP2/Scer\GAL4elav.PLu is a suppressor of neuron | third instar larval stage phenotype of KhcKO.mutA
Cele\unc-104::KhcLZ.UASp.mTagBFP2/Scer\GAL4elav.PLu is a suppressor of axon | third instar larval stage phenotype of KhcKO.mutA
Cele\unc-104::KhcLZ.UASp.mTagBFP2/Scer\GAL4elav.PLu is a suppressor of sensory neuron | third instar larval stage phenotype of KhcKO.mutA
Cele\unc-104::KhcLZ.UASp.mTagBFP2/Scer\GAL4elav.PLu is a suppressor of embryonic/larval optic stalk | third instar larval stage phenotype of KhcKO.mutA
The reduced velocity of ooplasmic streaming characteristic for oocytes expressing KhcGL00330 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 can be rescued by co-expression of Cele\unc-104::KhcLZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2.
The reduced axon length observed in cultured neurons from KhcKO.mutA mutant larvae as well as the axon outgrowth and patterning of photoreceptor neurons in KhcKO.mutA larval brains are rescued by expression of Cele\unc-104::KhcLZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2 under the control of Scer\GAL4elav.PLu in the mutant background.