FlyBase Allele Report: Dmel\Khc[KO.mutA]

General Information

Symbol

Dmel\Khc^KO.mutA

Species

D. melanogaster

Name

FlyBase ID

FBal0326706

Feature type

allele

Associated gene

Dmel\Khc

Associated Insertion(s)

TI{TI}Khc^KO.mutA

Carried in Construct

Also Known As

Khc^mutA

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Winding et al., 2016)

Progenitor genotype

Khc^KO.attP

(Winding et al., 2016)

Associated Insertion(s)

TI{TI}Khc^KO.mutA

Cytology

Description

Re-integration of mutated Khc (R914A, K915A, R916A and Q918A mutations in the C-terminal microtubule binding site) into the attP site present in Khc^KO.attP.

(Winding et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

complex_substitution

2R:16,267,438..16,267,452 [-]

Comment:

Four residues (R914A, K915A, R916A, and Q918A) were mutated in the C-terminal MT-binding site of Khc and re-integrated into the phiC31/attP site present in @Khc[KO.attP].

(Winding et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage

(Winding et al., 2016)

abnormal locomotor behavior | third instar larval stage

(Winding et al., 2016)

abnormal neuroanatomy | third instar larval stage

(Winding et al., 2016)

abnormal pain response | third instar larval stage

(Winding et al., 2016)

partially lethal - majority die | maternal effect

(Winding et al., 2016)

partially lethal - majority live

(Winding et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Khc^KO.mutA third instar larval adipocytes do not show defects in the normal perinuclear distribution of microtubules, nor in nuclear size or positioning.

(Sun et al., 2019)

The mitochondria distribution in Khc^KO.mutA mutant early stage egg chambers is not significantly different from controls but the ooplasmic streaming rate in the mutant oocytes is reduced, the motility of the free (unanchored) cytoplasmic microtubules is decreased and the posterior pole localization of stau protein is disrupted, however the nucleus localization at the anterodorsal corner of the oocyte is not perturbed.

(Lu et al., 2016)

Khc^KO.mutA homozygous flies from Khc^KO.mutA heterozygous parents display nearly 50% lethality before adulthood, however homozygous mutant progeny from the Khc^KO.mutA homozygous mothers is almost fully lethal with the strongest effect in the embryonic stage and even the heterozygous progeny show strongly reduced viability. Khc^KO.mutA mutant larvae as well as adults show severe locomotion defects (reduced velocity of locomotion).

Cultured neurons from Khc^KO.mutA homozygous as well as heterozygous mutant larvae exhibit reduced microtubule sliding and axon length but show no defects in mitochondria and peroxisomes transport. Khc^KO.mutA larval brain display severe loss of photoreceptor neuron axons targeting the lamina, the optic stalk is significantly thinner and the dendritic arbor of class IV or class I dendritic arborizing neurons contain fewer branches and the total dendrite length is strongly reduced compared to controls. Khc^KO.mutA mutant larvae also show decreased nociception.

(Winding et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Khc^KO.mutA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Cele\unc-104::Khc^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

NOT suppressed by

Statement

Reference

Khc^KO.mutA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Cele\unc-104^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

Khc^KO.mutA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Cele\unc-104::Khc^{LZ.mutA.UASp.mTagBFP2}

(Winding et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Khc^KO.mutA has sensory neuron | third instar larval stage phenotype, suppressible by Cele\unc-104::Khc^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

Khc^KO.mutA has embryonic/larval optic stalk | third instar larval stage phenotype, suppressible by Cele\unc-104::Khc^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

Khc^KO.mutA has neuron | third instar larval stage phenotype, suppressible by Cele\unc-104::Khc^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

Khc^KO.mutA has axon | third instar larval stage phenotype, suppressible by Cele\unc-104::Khc^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

NOT suppressed by

Statement

Reference

Khc^KO.mutA has neuron | third instar larval stage phenotype, non-suppressible by Cele\unc-104^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

Khc^KO.mutA has axon | third instar larval stage phenotype, non-suppressible by Cele\unc-104^{LZ.UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

Khc^KO.mutA has neuron | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Cele\unc-104::Khc^{LZ.mutA.UASp.mTagBFP2}

(Winding et al., 2016)

Khc^KO.mutA has axon | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Cele\unc-104::Khc^{LZ.mutA.UASp.mTagBFP2}

(Winding et al., 2016)

Khc^KO.mutA has sensory neuron | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Cele\unc-104::Khc^{LZ.mutA.UASp.mTagBFP2}

(Winding et al., 2016)

Khc^KO.mutA has embryonic/larval optic stalk | third instar larval stage phenotype, non-suppressible by Scer\GAL4^elav.PLu/Cele\unc-104::Khc^{LZ.mutA.UASp.mTagBFP2}

(Winding et al., 2016)

Suppressor of

Statement

Reference

Khc^KO.mutA, Scer\GAL4^{SPARC-MI00329-GAL4} is a suppressor of fat cell | third instar larval stage phenotype of Scer\GAL4^{SPARC-MI00329-GAL4}, shot^GL01286

(Sun et al., 2019)

Khc^KO.mutA, Scer\GAL4^{SPARC-MI00329-GAL4} is a suppressor of microtubule | third instar larval stage phenotype of Scer\GAL4^{SPARC-MI00329-GAL4}, shot^GL01286

(Sun et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The reduced axon length observed in cultured neurons from Khc^KO.mutA mutant larvae is rescued by expression of Cele\unc-104::Khc^{LZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2} (but not Cele\unc-104^{LZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2} or Cele\unc-104::Khc^{LZ.mutA.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2}) under the control of Scer\GAL4^elav.PLu in the mutant background. Similarly, Scer\GAL4^elav.PLu-driven expression of Cele\unc-104::Khc^{LZ.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2} is significantly improve the axon outgrowth and patterning of photoreceptor neurons in Khc^KO.mutA larval brains, while expression of Cele\unc-104::Khc^{LZ.mutA.Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2} cannot rescue the defects.

(Winding et al., 2016)

Complementation and Rescue Data

Rescued by

Khc^KO.mutA is rescued by Scer\GAL4^{VP16.nanos.UTR}/Khc^{UASp.mTagBFP2}

(Lu et al., 2016)

Partially rescued by

Khc^KO.mutA is partially rescued by Khc^{UASp.mTagBFP2}/Scer\GAL4^elav.PLu

(Winding et al., 2016)

Comments

The reduced velocity of ooplasmic streaming characteristic for Khc^KO.mutA mutant oocytes is fully rescued by expression of Khc^{Scer\UAS.P\T.T:Equa\eqFP578-TagBFP2} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the mutant background.

(Lu et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

79036

y¹ w^*; TI{TI}Khc^KO.mutA/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Khc^KO.mutA

(Zajac and Horne-Badovinac, 2022)

Khc^KO;mutA

Khc^mutA

(Sun et al., 2019, Lu et al., 2018, Lu et al., 2016, Winding et al., 2016)

khc^mut.A

(Hannaford et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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