Imprecise excision of P{EP}Atg16G5082, resulting in a 1178bp deletion extending from the original insertion site (revision of data reported in FBrf0235789).
Inferred boundaries of a 1178 bp deletion resulting from the imprecise excision of P{EP}Atg16G5082 that extends from the insertion site into Atg16.
marginal nerve (with Atg16d67)
The adult gut of Atg16d129 homozygotes do not show any severe histological abnormalities, including changes in length, diameter, epithelium thickness or peritrophic membrane thickness, as compared to controls; the epithelium, however, shows a significant increase in the proportion of pre-enteroendocrine cells and a reciprocal significant decrease in the proportion of mature enteroendocrine cells, but does not show significant changes in the pre-enteroendocrine cell maturation time, in the overall number of Prospero-positive precursor/mature enteroendocrine cells, in the proportion of mitotic or apoptotic cells, or in cell adhesion, as compared to controls.
Atg16d129 homozygous adults show a significant increase in the overall gut bacteria burden (but not in the numbers of Lactobacilli, Enterobacteriaceae or Acetobacteriaceae) in non-infected conditions, but show no significant changes in bacteria burden following oral infection with Pseudomonas aeruginosa or Enterococcus faecalis, as compared to controls. These mutants do not show significant changes in food uptake.
Starvation-induced formation of acidic autolysosomes (visualized by LysoTracker staining) is blocked in Atg16d129 fat cell somatic clones, unlike in the surrounding control cells in mosaic third instar larvae. Atg16d129 mutant adults show reduced climbing ability in the negative geotaxis assay compared to controls.
Atg16d129, Rab19[+]/Rab19LL00490 has increased cell number | adult stage phenotype
Atg16d129, Rab19[+]/Rab19LL00490 has adult posterior midgut epithelium phenotype
Atg16d129, Rab19[+]/Rab19LL00490 has enteroblast | adult stage phenotype
Atg16d129, Rab19[+]/Rab19LL00490 has enteroendocrine cell | adult stage phenotype
Atg16d129, Rab19LL00490 double heterozygotes exhibit a significant increase in the number of Prospero-positive precursor/mature enteroendocrine cells in the adult gut, as compared to the single heterozygotes or wild-type controls.
Atg16d129 is rescued by Atg16UAS.c.Tag:FLAG/Scer\GAL4esg.PU
Atg16d129 is rescued by Atg16ΔAutD.UAS/Scer\GAL4esg.PU
Atg16d129 is rescued by Atg16ΔAutD+Linker.UAS/Scer\GAL4esg.PU
Atg16d129 is rescued by Atg16ΔWD40.UAS/Scer\GAL4esg.PU
Both the increased proportion of pre-enteroendocrine cells and the reciprocal decreased proportion of mature enteroendocrine cells observed in the adult gut of Atg16d129 homozygotes are fully suppressed by the expression of Atg16Scer\UAS.c.T:Zzzz\FLAG, Atg16ΔAutD.Scer\UAS, Atg16ΔAutD+Linker.Scer\UAS, or Atg16ΔWD40.Scer\UAS, under the control of Scer\GAL4esg.PU.
