FlyBase Allele Report: Dmel\mad2[UAS.cLa]

General Information

Symbol

Dmel\mad2^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Liu

FlyBase ID

FBal0324943

Feature type

allele

Associated gene

Dmel\mad2

Associated Insertion(s)

Carried in Construct

P{UAS-mad2.L}

Key Links

Transgenic product class

cDNA

(Liu et al., 2015)

wild_type

(Liu et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Liu et al., 2015)

wild_type

(Liu et al., 2015)

Mutagen

in vitro construct

(Liu et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-mad2.L}

Cytology

Description

UASt regulatory sequences drive expression of a full-length mad2 cDNA.

(Liu et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mad2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of mad2^Scer\UAS.cLa alone under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} has no obvious effect on the germline stem cell population in adult testes, as compared to controls.

(Liu et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mad2^UAS.cLa, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of decreased cell number | adult stage phenotype of Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}/mad2^UAS.cLa is a suppressor | partially of decreased cell number | germline clone | adult stage phenotype of Mgtor^k03905

(Liu et al., 2015)

NOT Suppressor of

Statement

Reference

mad2^UAS.cLa, Scer\GAL4^C587 is a non-suppressor of increased cell number | adult stage phenotype of Mgtor^HMS00735, Scer\GAL4^C587

(Liu et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

mad2^UAS.cLa, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of testis | adult stage phenotype of Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

mad2^UAS.cLa, Scer\GAL4^{VP16.nanos.UTR} is a suppressor of male germline stem cell | adult stage phenotype of Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}/mad2^UAS.cLa is a suppressor | partially of male germline stem cell | adult stage phenotype of Mgtor^k03905

(Liu et al., 2015)

NOT Suppressor of

Statement

Reference

mad2^UAS.cLa, Scer\GAL4^C587 is a non-suppressor of testis | adult stage phenotype of Mgtor^HMS00735, Scer\GAL4^C587

(Liu et al., 2015)

mad2^UAS.cLa, Scer\GAL4^C587 is a non-suppressor of germinal proliferation center | adult stage phenotype of Mgtor^HMS00735, Scer\GAL4^C587

(Liu et al., 2015)

mad2^UAS.cLa, Scer\GAL4^C587 is a non-suppressor of male germline stem cell | adult stage phenotype of Mgtor^HMS00735, Scer\GAL4^C587

(Liu et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of mad2^Scer\UAS.cLa significantly rescues the decreased germline stem cell number defects present in the testis of adults expressing Mtor^GD13871 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

The increased number of germline stem/stem-like cells resulting from expression of Mtor^HMS00735 under the control of Scer\GAL4^C587 (in combination with Gal80[ts] to restrict expression to adulthood) is not suppressed by co-expressing mad2^Scer\UAS.cLa.

Expressing mad2^Scer\UAS.cLa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the germline stem cell loss seen in Mtor^k03905-homozygous germline clones within adult mosaic testis, 2 days after clone induction.

(Liu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mad2^Scer\UAS.cLa

mad2^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Liu

Secondary FlyBase IDs

References (1)

Report Sections

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