FlyBase Allele Report: Dmel\Mgtor[HMS00735]

General Information

Symbol

Dmel\Mgtor^HMS00735

Species

D. melanogaster

Name

FlyBase ID

FBal0257418

Feature type

allele

Associated gene

Dmel\Mgtor

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00735}

Also Known As

Mtor RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00735}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00735

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

increased cell number | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

Phenotype Manifest In

centrosome | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

cyst progenitor cell, with Scer\GAL4^NP1624

(Chen et al., 2013)

cyst progenitor cell | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

germinal proliferation center | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

germinal proliferation center | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

germline stem cell, with Scer\GAL4^NP1624

(Chen et al., 2013)

male germline cell, with Scer\GAL4^NP1624

(Chen et al., 2013)

male germline stem cell | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

male germline stem cell | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

mitotic spindle | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

testis | adult stage, with Scer\GAL4^C587

(Liu et al., 2015)

testis | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

wing, with Scer\GAL4^Bx-MS1096

(Liu et al., 2015)

Detailed Description

Statement

Reference

Expression of Mtor^HMS00735 driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in decreased numbers of germline stem cells in the adult germinal proliferation center, which become detached from the hub, as compared to controls; concomitantly, cyst somatic stem cells become associated with the hub.

Expression of Mtor^HMS00735 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} (in combination with Gal80[ts] to restrict expression during adulthood) results in mitotic germline stem cells presenting increased frequency of misoriented or multiple centrosomes, increased frequency of a range of mitotic spindle defects including misorientation, and increased frequency of chromosome segregation defects, as compared to controls.

Expression of Mtor^HMS00735 under the control of Scer\GAL4^C587 (in combination with Gal80[ts] to restrict expression to adulthood) results in a substantial increase in germline stem cells in the germinal proliferation center, and consequent localization of cyst somatic stem cells away from the hub, as compared to controls.

Expression of Mtor^HMS00735 under the control of Scer\GAL4^upd1.PU (in combination with Gal80[ts] to restrict expression to adulthood) does not lead to obvious defects in the germinal proliferation center, as compared to controls.

Expression of Mtor^HMS00735 driven by Scer\GAL4^Bx-MS1096 results in wing defects.

Expression of Mtor^HMS00735 under the control of Scer\GAL4^esg-NP5130 (in combination with Gal80[ts] to restrict expression to adulthood) does not have an obvious effect in the adult posterior midgut intestinal stem cells and progenitor population, nor does it affect the mitotic spindle orientation or chromosome segregation in adult posterior midgut intestinal stem cells, as compared to controls.

Expression of Mtor^HMS00735 under the control of either Scer\GAL4^byn-Gal4, Scer\GAL4^Cg.PA, Scer\GAL4^LE, Scer\GAL4^ppl.PU, Scer\GAL4^srp.Hemo, Scer\GAL4^Hml.PG or Scer\GAL4^Aug21 does not result in any obvious phenotype, as compared to controls.

(Liu et al., 2015)

Expression of Mtor^HMS00735 under the control of Scer\GAL4^tj-NP1624 results in an accumulation of early germ cells in the testis. The nuclei of many cyst stem cells in the mutant gonad are positioned adjacent to hub cells (in wild type the nuclei are dispersed away from the hub), while the number of germline stem cells associated with the hub is decreased compared to wild type.

(Chen et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Mgtor^HMS00735, Scer\GAL4^C587 has increased cell number | adult stage phenotype, non-suppressible by mad2^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Mgtor^HMS00735, Scer\GAL4^C587 has increased cell number | adult stage phenotype, non-suppressible by Mps1^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Suppressor of

Statement

Reference

Mgtor^HMS00735, Scer\GAL4^Su(H).GBE is a suppressor | partially of increased occurrence of cell division | adult stage phenotype of Myt1^KK102559, Scer\GAL4^Su(H).GBE

(Willms et al., 2020)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Mgtor^HMS00735, Scer\GAL4^C587 has testis | adult stage phenotype, non-suppressible by mad2^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Mgtor^HMS00735, Scer\GAL4^C587 has testis | adult stage phenotype, non-suppressible by Mps1^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Mgtor^HMS00735, Scer\GAL4^C587 has germinal proliferation center | adult stage phenotype, non-suppressible by mad2^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Mgtor^HMS00735, Scer\GAL4^C587 has germinal proliferation center | adult stage phenotype, non-suppressible by Mps1^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Mgtor^HMS00735, Scer\GAL4^C587 has male germline stem cell | adult stage phenotype, non-suppressible by mad2^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Mgtor^HMS00735, Scer\GAL4^C587 has male germline stem cell | adult stage phenotype, non-suppressible by Mps1^UAS.cLa, Scer\GAL4^C587

(Liu et al., 2015)

Suppressor of

Statement

Reference

Mgtor^HMS00735, Scer\GAL4^Su(H).GBE is a suppressor | partially of adult midgut phenotype of Myt1^KK102559, Scer\GAL4^Su(H).GBE

(Willms et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of germline stem cells resulting from the expression of Mtor^HMS00735 under the control of Scer\GAL4^C587 (in combination with Gal80[ts] to restrict expression to adulthood) is not suppressed by co-expressing mad2^Scer\UAS.cLa or Mps1^Scer\UAS.cLa.

Co-expression of shg^{Scer\UAS.T:Avic\GFP-rs} does not suppress the decreased germline stem cell number defects present in the testis of adults expressing Mtor^HMS00735 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Liu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

32941