FlyBase Allele Report: Hsap\MAPT[S2A.UAS.0N4R]

General Information

Symbol

Hsap\MAPT^S2A.UAS.0N4R

Species

H. sapiens

Name

FlyBase ID

FBal0324914

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-Tau.S2A.0N4R}

Also Known As

UAS-S2Atau

Key Links

Transgenic product class

missense_variant

(Ando et al., 2016)

polypeptide_post_translational_processing_variant

(Ando et al., 2016)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Ando et al., 2016)

polypeptide_post_translational_processing_variant

(Ando et al., 2016)

Mutagen

in vitro construct

(Ando et al., 2016)

Progenitor genotype

Carried in construct

P{UAS-Tau.S2A.0N4R}

Cytology

Description

UAS regulatory sequences drive expression of a form of Hsap\MAPT (human 0N4R tau, which has four tubulin-binding domains (R) at the C-terminal region and no N-terminal insert (N)) with alanine substitutions at Ser262 and Ser356, abolishing phosphorylation of these residues.

(Ando et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of tauopathy

CEA

(Oba et al., 2020, Ando et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of tauopathy

is exacerbated by Hsap\MARK4^{ΔG316E317D.UAS}

(Oba et al., 2020)

is exacerbated by Hsap\MARK4^UAS.cOa

(Oba et al., 2020)

is exacerbated by par-1^UAS.cSa

(Ando et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.PU

(Oba et al., 2020, Saito et al., 2019)

Phenotype Manifest In

adult brain, with Scer\GAL4^GMR.PU

(Saito et al., 2019)

lamina | adult stage, with Scer\GAL4^GMR.PU

(Oba et al., 2020)

Detailed Description

Statement

Reference

The expression of Hsap\MAPT^S2A.UAS.0N4R under the control of Scer\GAL4^GMR.PU results in neurodegeneration (i.e. brain vacuolization) in 10-days old adults.

(Saito et al., 2019)

The expression of Hsap\MAPT^{S2A.Scer\UAS.0N4R} under the control of Scer\GAL4^GMR.PU does not lead to frequent lamina vacuolization compared to controls.

(Oka et al., 2017)

Adults expressing Hsap\MAPT^{S2A.Scer\UAS.0N4R} driven by Scer\GAL4^GMR.PF do not show any obvious eye defects.

(Ando et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage phenotype, enhanceable by Hsap\MARK4^{ΔG316E317D.UAS}, Scer\GAL4^GMR.PU

(Oba et al., 2020)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Hsap\MARK4^UAS.cOa, Scer\GAL4^GMR.PU

(Oba et al., 2020)

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Cdk5^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Saito et al., 2019)

NOT suppressed by

Statement

Reference

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Cdk5^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Saito et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has lamina | adult stage phenotype, enhanceable by Hsap\MARK4^{ΔG316E317D.UAS}, Scer\GAL4^GMR.PU

(Oba et al., 2020)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has lamina | adult stage phenotype, non-enhanceable by Hsap\MARK4^UAS.cOa, Scer\GAL4^GMR.PU

(Oba et al., 2020)

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has adult brain phenotype, non-enhanceable by Cdk5^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Saito et al., 2019)

NOT suppressed by

Statement

Reference

Hsap\MAPT^S2A.UAS.0N4R, Scer\GAL4^GMR.PU has adult brain phenotype, non-suppressible by Cdk5^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Saito et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of Hsap\MAPT^{S2A.Scer\UAS.0N4R} and CaMKII^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PU does not lead to frequent lamina vacuolization compared to controls.

(Oka et al., 2017)

Co-expression of Hsap\APP^{Aβ42.Scer\UAS.T:Rnor\SS-PENK} and Hsap\MAPT^{S2A.Scer\UAS.0N4R} under the control of Scer\GAL4^GMR.PF does not seem to produce obvious eye defects.

(Ando et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\MAPT^{S2A.Scer\UAS.0N4R}

Hsap\MAPT^S2A.UAS.0N4R

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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