FB2024_03 , released June 25, 2024
Allele: Dmel\Cdk5UAS.Tag:FLAG
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General Information
Symbol
Dmel\Cdk5UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0117900
Feature type
allele
Associated gene
Dmel\Cdk5
Associated Insertion(s)
Carried in Construct
P{UAS-Cdk5-FLAG}
Also Known As
UAS-Cdk5, UAS-cdk5-FLAG
Key Links
Transgenic product class
cDNA
(Connell-Crowley, 2000)
wild_type
(Connell-Crowley, 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Connell-Crowley, 2000)
wild_type
(Connell-Crowley, 2000)
Mutagen
in vitro construct
(Connell-Crowley, 2000)
Progenitor genotype
Carried in construct
P{UAS-Cdk5-FLAG}
Cytology
Description

UAS sequences drive expression of the Cdk5α cDNA tagged with Tag:FLAG.

(Connell-Crowley, 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Cdk5
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  tauopathy
      modeled by Hsap\MAPTUAS.cWa
      (Saito et al., 2019)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Cdk5UAS.Tag:FLAG under the control of Scer\GAL4GMR.PU does not induce neurodegeneration (i.e. brain vacuolization) in 10-days old adults.

      (Saito et al., 2019)

      Expression of Cdk5Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4GMR.PF has no effect on eye surface morphology. Expression of Cdk5Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4unspecified has no effect on neuron cell fate.

      (Connell-Crowley et al., 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      NOT Enhancer of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of both Cdk5Scer\UAS.T:Zzzz\FLAG and Cdk5αScer\UAS.cMa under the control of Scer\GAL4GMR.PF results in a mild rough eye phenotype.

      (Shulman and Feany, 2003)

      When Cdk5αScer\UAS.T:Hsap\MYC and Cdk5Scer\UAS.T:Zzzz\FLAG are driven by Scer\GAL4elav-C155, axon patterning defects are seen. SNa axons are seen lacking the dorsal branch, with ectopic muscle contacts. Overextended and misrouted TN neurons and stalled ISN neurons are also seen. When Cdk5αScer\UAS.T:Hsap\MYC and Cdk5Scer\UAS.T:Zzzz\FLAG are driven by Scer\GAL4GMR.PF, a severely disrupted eye morphology results. In embryos, the proportion seen with nervous system defects increases.

      (Connell-Crowley et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Cdk5Scer\UAS.T:Zzzz\FLAG alone does not modify the rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF. Co-expression of both Cdk5Scer\UAS.T:Zzzz\FLAG and Cdk5αScer\UAS.cMa together strongly enhances the rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF.

      (Shulman and Feany, 2003)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Cdk5Scer\UAS.T:Zzzz\FLAG
      Cdk5UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (7)