FlyBase Allele Report: Dmel\pnt[P1-90]

General Information

Symbol

Dmel\pnt^P1-90

Species

D. melanogaster

Name

FlyBase ID

FBal0322762

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

Carried in Construct

Also Known As

pntP1⁹⁰

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Xie et al., 2016)

Mutagen

CRISPR/Cas9

(Xie et al., 2016)

Progenitor genotype

Cytology

Description

Mutation affecting the pnt-P1 isoform. Indel in the P1-specific exon 1, resulting in a frameshift and premature stop codon that deletes the 445 C-terminal amino acids (including the Ets DNA-binding domain).

(Xie et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:23,293,995..23,294,007 [-]

Comment:

Appears to be a deletion of 13 bp and insertion of 8 (CTGCGACT) after amino acid 176 causing a net deletion of 5 bp. Leads to a frameshift and early translation termination.

Inserted_sequence:

CTGCGACT

(Xie et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

intermediate neuronal progenitor | larval stage

(Xie et al., 2016)

intermediate neuronal progenitor | larval stage (with pnt^Δ88)

(Xie et al., 2016)

type II neuroblast | larval stage

(Xie et al., 2016)

type II neuroblast | larval stage (with pnt^Δ88)

(Xie et al., 2016)

Detailed Description

Statement

Reference

pnt^P1-90/pnt^P1-90 mutants are late third instar lethal.

pnt^P1-90/pnt^P1-90 or pnt^P1-90/pnt^Δ88 mutant larval brains have a significant increase in the number of Asense-negative type II neuroblasts, but a significant decrease in the number of intermediate neural progenitors.

(Xie et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pnt^P1-90 has decreased cell number | larval stage phenotype, enhanceable by btd[+]/btd^XG

(Xie et al., 2016)

pnt^P1-90 has abnormal neuroanatomy | larval stage phenotype, enhanceable by btd[+]/btd^XG

(Xie et al., 2016)

pnt^P1-90 has decreased cell number | larval stage phenotype, enhanceable by btd[+]/btd^MD808

(Xie et al., 2016)

pnt^P1-90 has abnormal neuroanatomy | larval stage phenotype, enhanceable by btd[+]/btd^MD808

(Xie et al., 2016)

pnt^P1-90 has increased cell number | larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

pnt^P1-90 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Suppressed by

Statement

Reference

pnt^P1-90 has increased cell number | larval stage phenotype, suppressible by btd[+]/btd^XG

(Xie et al., 2016)

pnt^P1-90 has increased cell number | larval stage phenotype, suppressible by btd[+]/btd^MD808

(Xie et al., 2016)

pnt^P1-90 has increased cell number | larval stage phenotype, suppressible by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Xie et al., 2016)

pnt^P1-90 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Xie et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

pnt^P1-90 has intermediate neuronal progenitor | larval stage phenotype, enhanceable by btd[+]/btd^XG

(Xie et al., 2016)

pnt^P1-90 has intermediate neuronal progenitor | larval stage phenotype, enhanceable by btd[+]/btd^MD808

(Xie et al., 2016)

pnt^P1-90 has type II neuroblast | larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Suppressed by

Statement

Reference

pnt^P1-90 has type II neuroblast | larval stage phenotype, suppressible | partially by btd[+]/btd^XG

(Xie et al., 2016)

pnt^P1-90 has type II neuroblast | larval stage phenotype, suppressible | partially by btd[+]/btd^MD808

(Xie et al., 2016)

pnt^P1-90 has type II neuroblast | larval stage phenotype, suppressible by Scer\GAL4^GMR9D11/erm^UAS.Tag:HA

(Xie et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

btd^XG/+ or btd^MD808/+ enhances the loss of intermediate neural progenitors, but suppresses the increased number of Asense-negative type II neuroblasts, although (in the case of btd^MD808/+) does not suppress the increased number of total neuroblasts seen in pnt^P1-90/pnt^P1-90 mutant larvae.

Df(2L)erm²/+ significantly enhances the increase in type II neuroblasts seen in pnt^P1-90/pnt^P1-90 mutant larvae.

Expression of erm^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR9D11 significantly suppresses the increase in type II neuroblasts seen in pnt^P1-90/pnt^P1-90 mutant larvae.

(Xie et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pnt^P1-90

pntP1⁹⁰

(Chen et al., 2021, Xie et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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