FlyBase Allele Report: Dmel\Nprl3[1]

General Information

Symbol

Dmel\Nprl3¹

Species

D. melanogaster

Name

FlyBase ID

FBal0319815

Feature type

allele

Associated gene

Dmel\Nprl3

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Cai et al., 2016)

Progenitor genotype

Cytology

Description

Deletion that removes most of the Nprl3 coding region. Sequence encoding amino acids 61-559 is deleted, and a frameshift is generated, resulting in a new stop codon after 5 amino acid residues.

(Cai et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:13,999,893..14,001,387 [-]

Comment:

Approximate boundaries of a deletion that extends from amino acid 61-559 of Nprl3. The deletion causes a frameshift which leads to translation termination after 5 amino acids.

(Cai et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chamber | germline clone

(Wei et al., 2016)

fat body | third instar larval stage | nutrition conditional (with Df(3L)ED4515)

(Wei et al., 2016)

follicle cell | somatic clone

(Wei et al., 2016)

Detailed Description

Statement

Reference

A high percentage of Nprl3¹/Nprl3¹ females die as pupae or pharate adults.

(Cai et al., 2016)

Nprl3¹ as well as Nprl3¹/Df(3L)ED4515 mutants are partially lethal (majority die at the pupae/pharate adult stage) but the Nprl3¹/Df(3L)ED4515 transheterozygotes eclose at significantly higher rate (though majority die as pharate adults) than the Nprl3¹ homozygotes - likely due to additional negative impact of their genetic background. Nprl3¹/Df(3L)ED4515 adults show reduced climbing ability.

The size of cells in the Nprl3¹ somatic follicle cell clones is significantly increased compared to adjacent heterozygous cells and the egg chambers containing Nprl3¹ mutant germline clones are also increased in size.

Nprl3¹ mutants - Nprl3¹/Df(3L)ED4515 transheterozygotes or homozygotes - display reduced survival rates under nutrient-liming conditions (complete starvation or amino acid starvation, respectively) and fail to activate autophagy (assessed by LysoTracker staining in larval fat body) upon starvation.

(Wei et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Nprl3¹ has partially lethal - majority die phenotype, non-enhanceable by Nprl2¹

(Wei et al., 2016)

Suppressed by

Statement

Reference

Df(3L)ED4515/Nprl3¹ has partially lethal - majority die phenotype, suppressible | partially by Tor[+]/mTor^A948V

(Wei et al., 2016)

NOT Enhancer of

Statement

Reference

Nprl3¹ is a non-enhancer of partially lethal - majority die phenotype of Nprl2¹

(Wei et al., 2016)

Phenotype Manifest In

Other

Statement

Reference

Nprl3¹, Wdr24¹ has embryonic/larval fat body | third instar larval stage phenotype

(Cai et al., 2016)

Nprl3¹, Wdr24¹ has lysosome | third instar larval stage phenotype

(Cai et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Fat bodies from Wdr24¹/Wdr24¹;Nprl3¹/Nprl3¹ third instar larvae accumulate large numbers of late endosomes or lysosomes (not autolysosomes); these are not observed in single Nprl3¹/Nprl3¹ mutants, and autolysosomes in Wdr24¹/Wdr24¹ single mutants are larger (and activate autophagy) rather than the than puncta in the double mutants (do not activate autophagy).

(Cai et al., 2016)

The partial lethality of either Nprl2¹ or Nprl3¹ single mutants is not increased further in the Nprl2¹;Nprl3¹ double mutants but can be significantly suppressed by combination with a single copy of Tor^A948V.

(Wei et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3L)ED4515/Nprl3¹ is rescued by Scer\GAL4^da.G32/Nprl3^{UASp.Tag:FLAG,Tag:HA}

(Wei et al., 2016)

Nprl3¹ is rescued by Scer\GAL4^da.G32/Nprl3^{UASp.Tag:FLAG,Tag:HA}

(Wei et al., 2016)

Nprl3¹ is rescued by Scer\GAL4^Cg.PA/Nprl3^{UASp.Tag:FLAG,Tag:HA}

(Wei et al., 2016)

Partially rescued by

Df(3L)ED4515/Nprl3¹ is partially rescued by Scer\GAL4^G14/Nprl3^{UASp.Tag:FLAG,Tag:HA}

(Wei et al., 2016)

Df(3L)ED4515/Nprl3¹ is partially rescued by Nprl3^{UASp.Tag:FLAG,Tag:HA}/Scer\GAL4^elav.PLu

(Wei et al., 2016)

Comments

The partial lethality as well as reduced survival rates and failure to activate autophagy (assessed by LysoTracker staining in larval fat body) under nutrient-limiting conditions characteristic for Nprl3¹ mutants (Nprl3¹ homozygotes or Nprl3¹/Df(3L)ED4515 transheterozygotes) is rescued by expression of Nprl3^{Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1} under the control of Scer\GAL4^da.G32. Expression under the Scer\GAL4^Cg.PA driver also rescues the increased lethality and climbing defects of Nprl3¹/Df(3L)ED4515 animals and the latter is partially suppressed also by Scer\GAL4^G14 and Scer\GAL4^elav.PLu-driven expression of Nprl3^{Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1}.

(Wei et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nprl3¹

nprl3¹

(Zhang et al., 2023, Zhou et al., 2021, Cheng et al., 2020, Wei et al., 2019, Cai et al., 2016, Wei et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (6)

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