FB2024_03 , released June 25, 2024
Allele: Dmel\Nprl3UASp.Tag:FLAG,Tag:HA
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General Information
Symbol
Dmel\Nprl3UASp.Tag:FLAG,Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0325030
Feature type
allele
Associated gene
Dmel\Nprl3
Associated Insertion(s)
Carried in Construct
P{UASp-Nprl3.FLAG.HA}
Also Known As
UAS-Nprl3
Key Links
Transgenic product class
wild_type
(Wei et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Wei et al., 2016)
Mutagen
in vitro construct
(Wei et al., 2016)
Progenitor genotype
Carried in construct
P{UASp-Nprl3.FLAG.HA}
Cytology
Description

UASp regulatory sequences drive expression of Nprl3 with N-terminal 3x-Tag:FLAG and 3x-Tag:HA tags.

(Wei et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Nprl3
Tagged with
Tag:FLAG
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4da.G32/Nprl3UASp.Tag:FLAG,Tag:HA rescues Df(3L)ED4515/Nprl31

      (Wei et al., 2016)

      Scer\GAL4da.G32/Nprl3UASp.Tag:FLAG,Tag:HA rescues Nprl31

      (Wei et al., 2016)

      Scer\GAL4Cg.PA/Nprl3UASp.Tag:FLAG,Tag:HA rescues Nprl31

      (Wei et al., 2016)
      Partially rescues

      Scer\GAL4G14/Nprl3UASp.Tag:FLAG,Tag:HA partially rescues Df(3L)ED4515/Nprl31

      (Wei et al., 2016)

      Nprl3UASp.Tag:FLAG,Tag:HA/Scer\GAL4elav.PLu partially rescues Df(3L)ED4515/Nprl31

      (Wei et al., 2016)
      Comments

      The partial lethality as well as reduced survival rates and failure to activate autophagy (assessed by LysoTracker staining in larval fat body) under nutrient-limiting conditions characteristic for Nprl31 mutants (Nprl31 homozygotes or Nprl31/Df(3L)ED4515 transheterozygotes) is rescued by expression of Nprl3Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4da.G32. Expression under the Scer\GAL4Cg.PA driver also rescues the increased lethality and climbing defects of Nprl31/Df(3L)ED4515 animals and the latter is partially suppressed also by Scer\GAL4G14 and Scer\GAL4elav.PLu-driven expression of Nprl3Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1.

      (Wei et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Nprl3Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1
      Nprl3UASp.Tag:FLAG,Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (3)