FlyBase Allele Report: Dmel\asp[t25]

asp^t25/Df(3R)BSC519 transheterozygous adults exhibit a smaller brain, due to a smaller and disorganized optic lobe, but not central brain, as compared to asp^t25 heterozygous controls; the size and morphology of the lamina and the retina are severely compromised, and the ocelli were reduced in size, extremely disorganized or completely absent. The addition of either asp^ASH.GFP or asp^C.GFP does not rescue the optic lobe defect and even leads to a smaller central brain, as compared to controls.

In asp^t25/Df(3R)BSC519 transheterozygotes, the cardia is reduced in volume, the midgut epithelium is thinner and the villi-like structures has altered morphology; egg chambers within the ovary are defective, consisting of only a few early-stage oocytes; there are no obvious defects in the heart, flight muscles or hindgut.

Both asp^t25/Df(3R)BSC519 transheterozygotes and asp^t25 heterozygotes do not show changes in thorax width, as compared to controls.

(Schoborg et al., 2019)

asp^t25/asp^t25 or asp^t25/Df(3R)BSC519 adult flies are viable but sterile, with small heads compared to asp^t25 heterozygotes. asp^t25/asp^t25 or asp^t25/Df(3R)BSC519 third instar larval central neuroblasts have unfocused spindle poles and detached centrosomes. Early stages of mitosis (up to and including nuclear envelope breakdown) proceed similar to wild type in asp^t25/Df(3R)BSC519 third instar larval central neuroblasts; shortly after nuclear envelope breakdown, centrosomes detach from the poles and move randomly around the cell - polarity establishment in these neuroblasts is not impaired and polarity is maintained through mitosis and associated with spindle poles, not wandering centrosomes. Some mother and daughter centrosomes go on to be correctly inherited, some swap positions before segregation, and in some cases both centrosomes are inherited by the neuroblast. Many asp^t25/Df(3R)BSC519 third instar larval central neuroblasts have an extended metaphase duration (though no increase in mitotic index and no change in neuroblast numbers), whereas others have near wild type timing; there are no cases of complete mitotic arrest.

(Schoborg et al., 2015)

External Data

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Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(3R)BSC519/asp^t25 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Scer\GAL4^elav.PLu/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has decreased size | adult stage phenotype, enhanceable by Scer\GAL4^elav.PLu/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Df(2L)Exel8005/Wdr62^Δ3-9

(Schoborg et al., 2019)

Df(3R)BSC519/asp^t25 has abnormal size | adult stage phenotype, enhanceable by Df(2L)Exel8005/Wdr62^Δ3-9

(Schoborg et al., 2019)

NOT Enhanced by

Statement

Reference

Df(3R)BSC519/asp^t25 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Wdr62[+]/Wdr62^Δ3-9

(Schoborg et al., 2019)

Df(3R)BSC519/asp^t25 has abnormal size | adult stage phenotype, non-enhanceable by Wdr62[+]/Wdr62^Δ3-9

(Schoborg et al., 2019)

Suppressed by

Statement

Reference

Df(3R)BSC519/asp^t25 has decreased size | adult stage phenotype, suppressible | partially by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has decreased size | adult stage phenotype, suppressible | partially by Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has decreased size | adult stage phenotype, suppressible | partially by Rel^E38/Dif¹

(Mannino et al., 2023)

NOT suppressed by

Statement

Reference

Df(3R)BSC519/asp^t25 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has decreased size | adult stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Other

Statement

Reference

Df(2L)Exel8005/Wdr62^Δ3-9, Df(3R)BSC519/asp^t25 has abnormal size | adult stage phenotype

(Schoborg et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Df(3R)BSC519/asp^t25 has adult brain phenotype, enhanceable by Scer\GAL4^elav.PLu/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe phenotype, enhanceable by Scer\GAL4^elav.PLu/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe phenotype, enhanceable by Df(2L)Exel8005/Wdr62^Δ3-9

(Schoborg et al., 2019)

NOT Enhanced by

Statement

Reference

Df(3R)BSC519/asp^t25 has neuropil | adult stage phenotype, non-enhanceable by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe neuron phenotype, non-enhanceable by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has medulla phenotype, non-enhanceable by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has neuropil | adult stage phenotype, non-enhanceable by Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe neuron phenotype, non-enhanceable by Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has medulla phenotype, non-enhanceable by Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has neuropil | adult stage phenotype, non-enhanceable by Rel^E38/Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe neuron phenotype, non-enhanceable by Rel^E38/Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has medulla phenotype, non-enhanceable by Rel^E38/Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe phenotype, non-enhanceable by Wdr62[+]/Wdr62^Δ3-9

(Schoborg et al., 2019)

Suppressed by

Statement

Reference

Df(3R)BSC519/asp^t25 has adult brain phenotype, suppressible | partially by Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult brain phenotype, suppressible | partially by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult brain phenotype, suppressible | partially by Rel^E38/Dif¹

(Mannino et al., 2023)

NOT suppressed by

Statement

Reference

Df(3R)BSC519/asp^t25 has neuropil | adult stage phenotype, non-suppressible by Rel^E38/Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe neuron phenotype, non-suppressible by Rel^E38/Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has medulla phenotype, non-suppressible by Rel^E38/Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult brain phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/BacA\p35^UAS.cHa

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has neuropil | adult stage phenotype, non-suppressible by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe neuron phenotype, non-suppressible by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has neuropil | adult stage phenotype, non-suppressible by Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has adult optic lobe neuron phenotype, non-suppressible by Dif¹

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has medulla phenotype, non-suppressible by Rel^E38

(Mannino et al., 2023)

Df(3R)BSC519/asp^t25 has medulla phenotype, non-suppressible by Dif¹

(Mannino et al., 2023)

Other

Statement

Reference

Df(2L)Exel8005/Wdr62^Δ3-9, Df(3R)BSC519/asp^t25 has adult central brain phenotype

(Schoborg et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

asp^t25/+, Wdr62^Δ3-9/Df(2L)Exel8005 and asp^t25/+, Wdr62^Δ3-9/+ adults do not show changes in optic lobe size, as compared to heterozygous controls; asp^t25/Df(3R)BSC519, Wdr62^Δ3-9/+ adults show a smaller optic lobe that is similar to asp^t25/Df(3R)BSC519 but does not show changes in the size of the central brain, as compared to controls; asp^t25/Df(3R)BSC519, Wdr62^Δ3-9/Df(2L)Exel8005 adults show a smaller optic lobe than asp^t25/Df(3R)BSC519 adults and a smaller central brain than controls.