FB2024_03 , released June 25, 2024
Allele: Dmel\Wdr62Δ3-9
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General Information
Symbol
Dmel\Wdr62Δ3-9
Species
D. melanogaster
Name
FlyBase ID
FBal0318556
Feature type
allele
Associated gene
Dmel\Wdr62
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Ramdas Nair et al., 2016)
Progenitor genotype
Cytology
Description

Deletion of part of Wdr62.

(Ramdas Nair et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  microcephaly
      CEA
      (Ramdas Nair et al., 2016)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  primary autosomal recessive microcephaly 5
      modeled by aspt25
      (Schoborg et al., 2019)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Wdr62Δ3-9/Df(2L)Exel8005 transheterozygous adult do not show changes in the size of the central brain or optic lobe, as compared to Wdr62Δ3-9 heterozygous controls.

      (Schoborg et al., 2019)

      Wdr62Δ3-9/Df(2L)Exel8005 is largely lethal at the pupal stage, with around 25% adult escapers; mutants are developmentally delayed.

      Loss of microtubule-organizing center (MTOC) activity on the apical centrosome during interphase leads to two centrioles devoid of microtubules in Wdr62Δ3-9/Df(2L)Exel8005 third instar larval neuroblasts.

      Wdr62Δ3-9/Df(2L)Exel8005 third instar larval neuroblasts display a centrosome asymmetry phenotype, with weaker microtubule intensity on maturing centrosome and metaphase spindles compared to wild type.

      Wdr62Δ3-9/Df(2L)Exel8005 third instar larval neuroblasts are more sensitive to cold, responding with an increased number of depolymerized microtubules during interphase and decreased microtubule regrowth following cold exposure compared to wild type.

      Wdr62Δ3-9/Df(2L)Exel8005 third instar larval brains are significantly smaller compared to wild type, with significantly reduced neuroblast numbers and significantly increased cell cycle length.

      (Ramdas Nair et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Wdr62[+]/Wdr62Δ3-9 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Df(3R)BSC519/aspt25

      (Schoborg et al., 2019)

      Wdr62[+]/Wdr62Δ3-9 is a non-enhancer of abnormal size | adult stage phenotype of Df(3R)BSC519/aspt25

      (Schoborg et al., 2019)
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Df(2L)Exel8005/Wdr62Δ3-9 is an enhancer of adult optic lobe phenotype of Df(3R)BSC519/aspt25

      (Schoborg et al., 2019)
      NOT Enhancer of
      Statement
      Reference

      Wdr62[+]/Wdr62Δ3-9 is a non-enhancer of adult optic lobe phenotype of Df(3R)BSC519/aspt25

      (Schoborg et al., 2019)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      aspt25/+, Wdr62Δ3-9/Df(2L)Exel8005 and aspt25/+, Wdr62Δ3-9/+ adults do not show changes in optic lobe size, as compared to heterozygous controls; aspt25/Df(3R)BSC519, Wdr62Δ3-9/+ adults show a smaller optic lobe that is similar to aspt25/Df(3R)BSC519 but does not show changes in the size of the central brain, as compared to controls; aspt25/Df(3R)BSC519, Wdr62Δ3-9/Df(2L)Exel8005 adults show a smaller optic lobe than aspt25/Df(3R)BSC519 adults and a smaller central brain than controls.

      (Schoborg et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Wdr62Δ3-9
      (Schoborg et al., 2019)
      wdr62Δ3-9
      (Gallaud et al., 2020, Ramdas Nair et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)