FlyBase Allele Report: Dmel\Strip[dogi]

General Information

Symbol

Dmel\Strip^dogi

Species

D. melanogaster

Name

doubled glomeruli

FlyBase ID

FBal0304737

Feature type

allele

Associated gene

Dmel\Strip

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Sakuma et al., 2014)

Mutagen

ethyl methanesulfonate

(Sakuma et al., 2014)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Sakuma et al., 2015)

abnormal neuroanatomy | somatic clone

(Sakuma et al., 2014)

abnormal size | male | third instar larval stage (with Strip^jam)

(La Marca et al., 2019)

lethal, with Df(3L)HR119

(Sakuma et al., 2014)

lethal, with Df(3L)HR232

(Sakuma et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Strip^jam/Strip^dogi mutants exhibit defective L3 male gonads: small gonads showing supernumerary strongly DAPI-positive cells throughout, whereas wild-type and Strip^jam/+ control gonads exhibit brighter DAPI-stained DNA in the apical region of the tissue, where the GSCs, gonialblasts, TA spermatogonia, CySCs and CyCs reside.

(La Marca et al., 2019)

Single-cell MARCM somatic clones of Strip^dogi homozygous mutant DL1 projection neurons display defects in dendrite branching in the mushroom body and axon elongation (axons do not elongate or form the stereotypical branches in the lateral horn).

Strip^dogi homozygous MARCM clones of mushroom body alpha/beta neurons display axon segregation phenotypes (less axons to the alpha lobe)

(Sakuma et al., 2015)

Mutant olfactory projection neurons (PNs) in neuroblast clones show severe defects in guidance and targeting, including ectopic innervation to the contralateral antennal lobe or the subesophageal ganglion.

Homozygous single-cell DL1 PN clones show defects in both the axons and dendrites. The axons do not elongate (98.2% penetrance) or form the normal L-shaped branches within the lateral horn. The dendrites have an additional branch appearing from the proximal side of the dendrite shaft (35.3% penetrance), resulting in targeting of two glomeruli instead of a single glomerulus.

(Sakuma et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Strip^dogi has abnormal neuroanatomy | adult stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^UAS.cOa

(Sakuma et al., 2015)

Strip^dogi has abnormal neuroanatomy | adult stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^RNAi.UAS.cSa

(Sakuma et al., 2015)

Strip^dogi has abnormal neuroanatomy | somatic clone phenotype, enhanceable | somatic clone by DCTN1-p150^Gl-1

(Sakuma et al., 2014)

Strip^dogi has abnormal neuroanatomy | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/spri^RNAi.UAS

(Sakuma et al., 2014)

Strip^dogi has abnormal neuroanatomy | somatic clone phenotype, enhanceable | somatic clone by Rab5^{S43N.UASp.YFP}/Scer\GAL4^GH146

(Sakuma et al., 2014)

Suppressed by

Statement

Reference

Strip^dogi has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Rab5^{Q88L.UASp.YFP}/Scer\GAL4^GH146

(Sakuma et al., 2014)

Strip^dogi has abnormal neuroanatomy | somatic clone phenotype, suppressible | somatic clone by Mmus\Strip1^UAS.cSa/Scer\GAL4^GH146

(Sakuma et al., 2014)

Suppressor of

Statement

Reference

Strip[+]/Strip^dogi is a suppressor of abnormal neuroanatomy | adult stage phenotype of Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107

(Sakuma et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Strip^dogi has mushroom body | adult stage phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^RNAi.UAS.cSa

(Sakuma et al., 2015)

Strip^dogi has adult lateral horn | adult stage phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^RNAi.UAS.cSa

(Sakuma et al., 2015)

Strip^dogi has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^UAS.cOa

(Sakuma et al., 2015)

Strip^dogi has axon | adult stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^UAS.cOa

(Sakuma et al., 2015)

Strip^dogi has dendrite | adult stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^UAS.cOa

(Sakuma et al., 2015)

Strip^dogi has mushroom body | adult stage phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^UAS.cOa

(Sakuma et al., 2015)

Strip^dogi has adult lateral horn | adult stage phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^UAS.cOa

(Sakuma et al., 2015)

Strip^dogi has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^RNAi.UAS.cSa

(Sakuma et al., 2015)

Strip^dogi has axon | adult stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^RNAi.UAS.cSa

(Sakuma et al., 2015)

Strip^dogi has dendrite | adult stage | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/Tbcd^RNAi.UAS.cSa

(Sakuma et al., 2015)

Strip^dogi has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, enhanceable | somatic clone by DCTN1-p150^Gl-1

(Sakuma et al., 2014)

Strip^dogi has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, enhanceable | somatic clone by Scer\GAL4^GH146/spri^RNAi.UAS

(Sakuma et al., 2014)

Strip^dogi has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, enhanceable | somatic clone by Rab5^{S43N.UASp.YFP}/Scer\GAL4^GH146

(Sakuma et al., 2014)

Suppressed by

Statement

Reference

Strip^dogi has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, suppressible | partially by Rab5^{Q88L.UASp.YFP}/Scer\GAL4^GH146

(Sakuma et al., 2014)

Strip^dogi has antennal lobe projection neuron | somatic clone phenotype, suppressible | somatic clone by Mmus\Strip1^UAS.cSa/Scer\GAL4^GH146

(Sakuma et al., 2014)

Strip^dogi has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, suppressible | somatic clone by Mmus\Strip1^UAS.cSa/Scer\GAL4^GH146

(Sakuma et al., 2014)

Suppressor of

Statement

Reference

Strip[+]/Strip^dogi is a suppressor of adult mushroom body alpha-lobe phenotype of Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107

(Sakuma et al., 2015)

Strip[+]/Strip^dogi is a suppressor of adult mushroom body beta-lobe phenotype of Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107

(Sakuma et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The dendrite branching and axon elongation phenotypes observed in single-cell MARCM somatic clones of Strip^dogi homozygous mutant DL1 projection neurons are significantly enhanced by expression of either TBCD^{dsRNA.shRNA.Scer\UAS} RNAi or TBCD^Scer\UAS.cOa in the clones under the control of the Scer\GAL4^GH146 driver. Some neurons show the 'extremely short axon' phenotype: the axon does not exit the antennal lobe at all.

The loss of alpha and beta lobes of the mushroom body observed in brains of adult flies expressing Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ey-OK107 is partially restored by combination with Strip^dogi in heterozygous state.

(Sakuma et al., 2015)

The axon elongation and dendrite branching defects seen in Strip^dogi DL1 olfactory projection neuron single-cell clones are more severe if the cells are also homozygous for Gl¹. The axon fails to exit the antennal lobe in 15.6% of cases.

The axon elongation and dendrite branching defects seen in Strip^dogi DL1 olfactory projection neuron single-cell clones are more severe if the cells are also expressing spri^{dsRNA.shRNA.Scer\UAS} under the control of Scer\GAL4^GH146. The axon fails to exit the antennal lobe in 13.0% of cases.

The axon elongation defect seen in Strip^dogi DL1 olfactory projection neuron single-cell clones is partially suppressed if the cells are also expressing Rab5^{Q88L.Scer\UAS.P\T.T:Avic\GFP-YFP} and is enhanced if the cells are also expressing Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} (expression driven by Scer\GAL4^GH146 in each case).

(Sakuma et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Strip1^Scer\UAS.cSa under the control of Scer\GAL4^GH146 in Strip^dogi olfactory projection neuron neuroblast clones rescues the defects normally seen in these mutant cells.

Expression of Mmus\Strip1^Scer\UAS.cSa under the control of Scer\GAL4^GH146 in Strip^dogi DL1 olfactory projection neuron single-cell clones rescues the axon and dendrite defects normally seen in these mutant cells.

(Sakuma et al., 2014)

Complementation and Rescue Data

Rescued by

Strip^dogi is rescued by Scer\GAL4^GH146/Strip^UAS.cSa

(Sakuma et al., 2014)

Comments

Expression of Strip^Scer\UAS.cSa under the control of Scer\GAL4^GH146 in Strip^dogi olfactory projection neuron neuroblast clones rescues the defects normally seen in these mutant cells.

Expression of Strip^Scer\UAS.cSa under the control of Scer\GAL4^GH146 in Strip^dogi DL1 olfactory projection neuron single-cell clones rescues the axon and dendrite defects normally seen in these mutant cells.

(Sakuma et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Strip^dogi

(La Marca et al., 2019)

strip^dogi

(Sakuma et al., 2015, Sakuma et al., 2014)

Name Synonyms

doubled glomeruli

(Sakuma et al., 2014)

Secondary FlyBase IDs

References (3)

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