FB2024_03 , released June 25, 2024
Allele: Dmel\His3HisTA.H3K36R
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General Information
Symbol
Dmel\His3HisTA.H3K36R
Species
D. melanogaster
Name
FlyBase ID
FBal0304395
Feature type
allele
Associated gene
His3
Associated Insertion(s)
Carried in Construct
PBac{12xHisTA.H3K36R}
Key Links
Transgenic product class
missense_variant
(McKay et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(McKay et al., 2015)
Mutagen
in vitro construct
(McKay et al., 2015)
Progenitor genotype
Carried in construct
PBac{12xHisTA.H3K36R}
Cytology
Description

Mutant His3 gene (amino acid replacement K36R) in the context of a histone gene repeat tandem array.

(McKay et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
His3
Encoded product / tool
His3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When carried in a wild type or hemizygous Df(2L)HisC mutant background, a transgene bearing 12 copies of a mutated form of the histone repeat unit (PBac{12xHisTA.H3K36R} - comprised of 12xHis3HisTA.H3K36R and 12x wild type copies of His1HisTA.wt, His2AHisTA.wt, His2BHisTA.wt and His3HisTA.wt) in a wild type background causes no discernible developmental or fertility defects. 100% of homozygous Df(2L)HisC mutant animals containing one PBac{12xHisTA.H3K36R} transgene die before the end of pupal development.

      (McKay et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      When separated from their heterozygous siblings at L1, His3HisTA.H3K36R, Df(2L)HisC/Df(2L)HisC individuals survive till pharate adult stage with some adults escaping to pupal case to die shortly after; in contrast, Df(2L)HisC homozygotes are embryo lethal. The His3HisTA.H3K36R, Df(2L)HisC/Df(2L)HisC escapers do not show homeotic transformations.

      (Dorafshan et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      His3HisTA.H3K36R
      Name Synonyms
      Secondary FlyBase IDs
        References (4)