FlyBase Allele Report: Dmel\TrpA1[ins]

General Information

Symbol

Dmel\TrpA1^ins

Species

D. melanogaster

Name

FlyBase ID

FBal0304384

Feature type

allele

Associated gene

Dmel\TrpA1

Associated Insertion(s)

TI{TI}TrpA1^ins

Carried in Construct

Also Known As

dTrpA1^ins

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-in gene targeting

(Hamada et al., 2008)

recombination

(Hamada et al., 2008)

Progenitor genotype

Associated Insertion(s)

TI{TI}TrpA1^ins

Cytology

Description

Tandem array of two mutated copies of TrpA1. One copy is deleted for the sixth transmembrane domain and carboxy terminus, whereas the other copy is deleted for the promoter region and start codon and contains a translational stop before the transmembrane domains). A w^+mW.hs marker and a single FRT site are located between the two copies.

(Hamada et al., 2008)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

AC thermosensory neuron

(Hamada et al., 2008)

intermediate labellar taste bristle

(Kang et al., 2012)

Detailed Description

Statement

Reference

TrpA1^ins third instar larvae exhibit significantly slower heat nociception when compared to controls.

(Khuong et al., 2019)

TrpA1^ins flies show lack of defecation in response to uracil or N-methylmaleimide.

Guts from TrpA1^ins flies exhibit increased colony-forming units 5 hours after feeding with the microbe Erwinia carotovora.

When fed wild-type Erwinia carotovora, TrpA1^ins flies display no change in mortality rate as compared to control flies.

(Du et al., 2016)

TrpA1^ins mutants exhibit normal light-dependent temperature preference. Similarly to wild-type, they prefer higher temperature in the light than in the dark.

(Head et al., 2015)

TrpA1^ins mutant adults have significantly reduced aversive temperature, but not shock, olfactory memory, compared to controls.

(Galili et al., 2014)

TrpA1^ins/TrpA1^ins does not significantly affect locomotor turning frequency in third instar larvae.

(Gorczyca et al., 2014)

Dp(3;3)TrpA1^ins mutant flies (comprised of the TrpA1^ins.5' and TrpA1^ins.3' alleles) show complete loss of the long term thermal preference (response to a shallow temperature gradient after 30 min) seen in wild type flies. The flies show a preference for temperatures >28[o]C.

TrpA1^ins mutant flies show complete loss of the long term thermal preference (response to a shallow temperature gradient after 30 min) seen in wild type flies. The flies show a preference for temperatures >28[o]C.

(Ni et al., 2013)

Berberine-sensitive i-type taste bristles in the labellum do not respond to N-methylmaleimide in an extracellular tip recording assay in TrpA1^ins flies (in contrast to wild type where robust spiking is induced). Warming to 39[o]C does not elicit spiking in these cells, both in mutant and wild-type flies.

TrpA1^ins flies show decreased avoidance of food containing N-methylmaleimide compared to wild type.

Berberine-sensitive i-type taste bristles in the labellum do not respond to N-methylmaleimide in an extracellular tip recording assay in Dp(3;3)TrpA1^ins flies (in contrast to wild type where robust spiking is induced). Warming to 39[o]C does not elicit spiking in these cells, both in mutant and wild-type flies.

Dp(3;3)TrpA1^ins flies show decreased avoidance of food containing N-methylmaleimide compared to wild type.

(Kang et al., 2012)

Homozygous mutants fail to avoid noxious temperature levels. There is no difference in temperature-induced paralysis between TrpA1^ins and wild-type flies subjected to 46[o]C. Mutant flies do not exhibit any coordination defects.

(Neely et al., 2011)

TrpA1^ins.5', TrpA1^ins.3' flies display a reduced aversion to aristolochic acid compared to wild type.

In contrast to wild type, TrpA1^ins.5', TrpA1^ins.3' flies have virtually no aristolochic acid-induced action potentials in tip recordings of sensillum s6.

TrpA1^ins flies display a reduced aversion to aristolochic acid compared to wild type.

In contrast to wild type, TrpA1^ins flies have virtually no aristolochic acid-induced action potentials in tip recordings of sensillum s6.

(Kim et al., 2010)

Dp(3;3)TrpA1^ins flies show greatly reduced avoidance of 1% citronellal in a direct airborne repellent test (DART) assay.

TrpA1^ins flies show greatly reduced avoidance of 1% citronellal in a direct airborne repellent test (DART) assay.

(Kwon et al., 2010)

TrpA1^ins flies show an altered distribution along a thermal gradient compared to wild-type flies. The mutant flies show increased accumulation in the warmest regions (28-32[o]C) of the gradient, but not in the coolest region (18-22[o]C).

TrpA1^ins/Df(3L)ED4415 flies show an altered distribution along a thermal gradient compared to wild-type flies. The mutant flies show increased accumulation in the warmest regions (28-32[o]C) of the gradient, but not in the coolest region (18-22[o]C).

Anterior cell (AC) neurons in the brains of TrpA1^ins flies do not show warmth-responsive increases in intracellular calcium, in contrast to control AC neurons.

Dp(3;3)TrpA1^ins flies (which carry TrpA1^ins.5' and TrpA1^ins.3') show an altered distribution along a thermal gradient compared to wild-type flies. The mutant flies show increased accumulation in the warmest regions (28-32[o]C) of the gradient, but not in the coolest region (18-22[o]C).

Df(3L)ED4415/Dp(3;3)TrpA1^ins flies show an altered distribution along a thermal gradient compared to wild-type flies. The mutant flies show increased accumulation in the warmest regions (28-32[o]C) of the gradient, but not in the coolest region (18-22[o]C).

Anterior cell (AC) neurons in the brains of Dp(3;3)TrpA1^ins flies do not show warmth-responsive increases in intracellular calcium, in contrast to control AC neurons.

(Hamada et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

TrpA1^ins has abnormal immune response phenotype, suppressible by Rel^E20

(Du et al., 2016)

TrpA1^ins has abnormal temperature response phenotype, suppressible | partially by Gr28b^UAS.D/Scer\GAL4^Gr28b.d.PT

(Ni et al., 2013)

TrpA1^ins has abnormal temperature response phenotype, suppressible by Gr28b^UAS.D/Scer\GAL4^Gr28b.d.PT

(Ni et al., 2013)

NOT Enhancer of

Statement

Reference

TrpA1^ins is a non-enhancer of short lived | nutrition conditional phenotype of Rel^E20

(Du et al., 2016)

Other

Statement

Reference

TrpA1[+]/TrpA1^ins, tor^e00150 has abnormal phototaxis | dominant | third instar larval stage phenotype

(Yamanaka et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Rel^E20 partially suppresses the increased colony-forming unit gut phenotype of TrpA1^ins.

Expression of TrpA1^+tHa rescues the uracil-dependent defecation defect seen in TrpA1^ins flies.

Expression of TrpA1^{Scer\UAS.(A).10b} under the control of Scer\GAL4^elav-C155 is unable to rescue the uracil-dependent defecation defect of TrpA1^ins mutants.

Expression of TrpA1^{Scer\UAS.(A).10b} under the control of Scer\GAL4^TrpA1.C-D rescues the uracil- and N-methylmaleimide- dependent defecation defects of TrpA1^ins mutants.

Expression of TrpA1^{Scer\UAS.(A).10b} under the control of Scer\GAL4^TrpA1.C-D is unable to rescue the uracil-dependent defecation defect, but is able to rescue the N-methylmaleimide-dependent defecation defect of TrpA1^ins mutants.

When fed Erwinia carotovora, TrpA1^ins and Rel^E20 double mutants display a significantly higher mortality rate than control flies, but not significantly higher than Rel^E20 mutants.

(Du et al., 2016)

Expression of Gr28b^Scer\UAS.D in the arista under the control of Scer\GAL4^Gr28b.d.PT significantly rescues the "rapid negative thermotaxis" defects seen in Dp(3;3)TrpA1^ins mutant flies (comprised of the TrpA1^ins.5' and TrpA1^ins.3' alleles).

Expression of Gr28b^Scer\UAS.D in the arista under the control of Scer\GAL4^Gr28b.d.PT significantly rescues the "rapid negative thermotaxis" defects seen in TrpA1^ins mutant flies.

(Ni et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

TrpA1^ins is rescued by Scer\GAL4^TrpA1.C-D/TrpA1^UAS.(A).10b

(Du et al., 2016)

Partially rescued by

TrpA1^ins is partially rescued by TrpA1^UAS.(A).cKa/Scer\GAL4^TrpA1.C-D

(Du et al., 2016)

Not rescued by

TrpA1^ins is not rescued by Scer\GAL4^elav-C155/TrpA1^UAS.(A).10b

(Du et al., 2016)

Comments

Expression of TrpA1^{Scer\UAS.(B).cKa} in the arista under the control of Scer\GAL4^Gr28b.d.PT rescues the "rapid negative thermotaxis" defects seen in TrpA1^ins mutant flies.

Expression of TrpA1^{Scer\UAS.(B).cKa} in the arista under the control of Scer\GAL4^Gr28b.d.PT rescues the "rapid negative thermotaxis" defects seen in Dp(3;3)TrpA1^ins mutant flies (comprised of the TrpA1^ins.5' and TrpA1^ins.3' alleles).

(Ni et al., 2013)

Expression of TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^Gr66a.PD restores an electrophysiological spiking response to N-methylmaleimide in berberine-sensitive i-type taste bristles in the labellum in Dp(3;3)TrpA1^ins flies. Sensitivity to warming is also restored.

Expression of TrpA1^{Scer\UAS.(A).cKa} under the control of Scer\GAL4^Gr66a.PD restores an electrophysiological spiking response to N-methylmaleimide in berberine-sensitive i-type taste bristles in the labellum in Dp(3;3)TrpA1^ins flies. Sensitivity to warming is not restored.

Expression of either TrpA1^{Scer\UAS.(A).cKa} or TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^Gr66a.PD rescues avoidance of food containing N-methylmaleimide in Dp(3;3)TrpA1^ins flies.

Expression of TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^Gr66a.PD restores an electrophysiological spiking response to N-methylmaleimide in berberine-sensitive i-type taste bristles in the labellum in TrpA1^ins flies. Sensitivity to warming is also restored.

Expression of TrpA1^{Scer\UAS.(A).cKa} under the control of Scer\GAL4^Gr66a.PD restores an electrophysiological spiking response to N-methylmaleimide in berberine-sensitive i-type taste bristles in the labellum in TrpA1^ins flies. Sensitivity to warming is not restored.

Expression of either TrpA1^{Scer\UAS.(A).cKa} or TrpA1^{Scer\UAS.(B).cKa} under the control of Scer\GAL4^Gr66a.PD rescues avoidance of food containing N-methylmaleimide in TrpA1^ins flies.

(Kang et al., 2012)

TrpA1^+tHa rescues the thermal avoidance defect found in TrpA1^ins mutant flies.

(Neely et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Dp(3;3)TrpA1^ins

TrpA1^ins.3'

TrpA1^ins.5'

TrpA1^ins

(Jin et al., 2021, Khuong et al., 2019, Khuong et al., 2019, Du et al., 2016, Du et al., 2016, Head et al., 2015, Ni et al., 2013, Kang et al., 2012, Neely et al., 2011, Hamada et al., 2008)

dTRPA1^KO

(Guntur et al., 2015)

dTRPA1^ins

(Das et al., 2016, Das et al., 2015)

dTrpA1^ins

(Terada et al., 2016, Galili et al., 2014, Gorczyca et al., 2014)

dtrpA1^ins

(Rosenzweig et al., 2008)

trpA1^ins

(Omelchenko et al., 2022, Kim et al., 2010, Kwon et al., 2010)

Name Synonyms

Secondary FlyBase IDs

FBal0220311
FBal0220310
FBab0045819

References (21)

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