FB2024_03 , released June 25, 2024
Allele: Scer\GAL4TrpA1.C-D
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General Information
Symbol
Scer\GAL4TrpA1.C-D
Species
S. cerevisiae
Name
FlyBase ID
FBal0267193
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{TrpA1-GAL4.C-D}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Zhong et al., 2012)
Progenitor genotype
Carried in construct
P{TrpA1-GAL4.C-D}
Cytology
Description

A 2.1kb region between the mfr gene and the transcription start site for the C and D isoforms of TrpA1 regulates expression of a GAL4 driver.

(Zhong et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
TrpA1
TrpA1_C/D-GAL4
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval multidendritic class IV neuron
      (Zhong et al., 2012)
      immunolocalization
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult midgut | restricted
      (Du et al., 2016)
      enteroendocrine cell
      (Du et al., 2016)
      enterocyte | subset
      (Du et al., 2016)
      Additional Information
      Statement
      Reference

      ScerGAL4TrpA1.C-D cells are enteroendocrine cells marked with anti-pros, except for large cells in the middle midgut that appear to be enterocytes .

      (Du et al., 2016)

      This Gal4 construct was designed to better capture upstream enhancer elements of TrpA1. In contrast to previously published reporters, ScerGAL4TrpA1.C-D shows expression nearly exclusively in in larval class IV dendritic arborizing neurons (mdIV nociceptors) and their central projections.

      (Zhong et al., 2012)
       
      Marker for
      Reflects expression of
      TrpA1
      (Zhong et al., 2012)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4TrpA1.C-D
      Name Synonyms
      Secondary FlyBase IDs
        References (4)