FlyBase Allele Report: Dmel\Tbcd[1]

General Information

Symbol

Dmel\Tbcd¹

Species

D. melanogaster

Name

FlyBase ID

FBal0302633

Feature type

allele

Associated gene

Dmel\Tbcd

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

A ~9kb long DNA fragment has been inserted into the coding sequence of the TBCD locus.

(Okumura et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of early onset progressive encephalopathy with brain atrophy and thin corpus callosum

CEC

(Miyake et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of early onset progressive encephalopathy with brain atrophy and thin corpus callosum

is ameliorated by Hsap\TBCD^UAS.cMa

(Miyake et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | cell autonomous | dominant | somatic clone

(Okumura et al., 2015)

abnormal neuroanatomy | cell autonomous | somatic clone

(Okumura et al., 2015)

abnormal neuroanatomy | cell non-autonomous | dominant

(Okumura et al., 2015)

abnormal neuroanatomy | somatic clone

(Miyake et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

TBCD¹ mutant olfactory projection neuron single-cell MARCM clones show a range of axon (from a short or missing dorsal axon branch at the lateral horn to extremely short axon) as well as dendrite (reduced in situ dendritic branching, ectopic branches or combination of the two) morphological defects.

(Miyake et al., 2016)

The dendrites of homozygous TBCD¹ mutant DL1 olfactory projection neurons (PNs) target not only the DL1 glomerulus, but also additional glomeruli. Although the dendrites of TBCD¹ DL1 PNs target the DL1 glomerulus, the number of dendrites innervation the DL1 glomerulus is decreased and there are ectopic arborizations between the cell body and DL1 glomerulus. The axons do not reach the mushroom body and the lateral horn. Ectopic dendrite arborization is first observed at 48 hours after puparium formation (APF). There are no detectable differences in axon morphology until 72 hours APF, but 1 day after eclosion the dorsal branch in the lateral horn is lost and the PN axons have developed swellings typical of axon degeneration.

In wild type DL1 projection neuron clones generated in a TBCD¹/+ background an increased number of dendrites spill over to the outside of the antennal lobe compared to controls

(Okumura et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Tbcd¹ has abnormal neuroanatomy | cell autonomous | somatic clone phenotype, enhanceable by Tbce^RNAi.UAS.cJa/Scer\GAL4^GH146

(Okumura et al., 2015)

Tbcd¹ has abnormal neuroanatomy | cell non-autonomous | dominant phenotype, enhanceable | cell autonomous | somatic clone by Dscam1⁰⁵⁵¹⁸

(Okumura et al., 2015)

Suppressed by

Statement

Reference

Tbcd¹ has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Hsap\TBCD^UAS.cMa/Scer\GAL4^GH146

(Miyake et al., 2016)

NOT suppressed by

Statement

Reference

Tbcd¹ has abnormal neuroanatomy | somatic clone | cell autonomous phenotype, non-suppressible by ds^UAS.cTa/Scer\GAL4^GH146

(Okumura et al., 2015)

Tbcd¹ has abnormal neuroanatomy | somatic clone | cell autonomous phenotype, non-suppressible by CG11723^UAS.cOa/Scer\GAL4^GH146

(Okumura et al., 2015)

Tbcd¹ has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by CG4259^UAS.cOa/Scer\GAL4^GH146

(Okumura et al., 2015)

NOT Enhancer of

Statement

Reference

TBCD[+]/Tbcd¹ is a non-enhancer of abnormal neuroanatomy | cell autonomous | somatic clone phenotype of Dscam1⁰⁵⁵¹⁸

(Okumura et al., 2015)

Suppressor of

Statement

Reference

TBCD[+]/Tbcd¹ is a suppressor of abnormal neuroanatomy phenotype of Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107

(Okumura et al., 2015)

Other

Statement

Reference

Hsap\TBCD^M387R.UAS, Scer\GAL4^GH146, Tbcd¹ has abnormal neuroanatomy | somatic clone phenotype

(Miyake et al., 2016)

Hsap\TBCD^R772C.UAS, Scer\GAL4^GH146, Tbcd¹ has abnormal neuroanatomy | somatic clone phenotype

(Miyake et al., 2016)

Hsap\TBCD^Y760stop.UAS, Scer\GAL4^GH146, Tbcd¹ has abnormal neuroanatomy | somatic clone phenotype

(Miyake et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Tbcd¹ has adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone phenotype, enhanceable by Tbce^RNAi.UAS.cJa/Scer\GAL4^GH146

(Okumura et al., 2015)

Tbcd¹ has adult antennal lobe projection neuron DL1 adPN | cell non-autonomous phenotype, enhanceable | cell autonomous | somatic clone by Dscam1⁰⁵⁵¹⁸

(Okumura et al., 2015)

Suppressed by

Statement

Reference

Tbcd¹ has adult antennal lobe projection neuron | somatic clone phenotype, suppressible | partially by Hsap\TBCD^UAS.cMa/Scer\GAL4^GH146

(Miyake et al., 2016)

Tbcd¹ has axon | somatic clone | adult stage phenotype, suppressible | partially by Hsap\TBCD^UAS.cMa/Scer\GAL4^GH146

(Miyake et al., 2016)

Tbcd¹ has axon collateral | somatic clone | adult stage phenotype, suppressible | partially by Hsap\TBCD^UAS.cMa/Scer\GAL4^GH146

(Miyake et al., 2016)

Tbcd¹ has dendrite | somatic clone | adult stage phenotype, suppressible | partially by Hsap\TBCD^UAS.cMa/Scer\GAL4^GH146

(Miyake et al., 2016)

NOT suppressed by

Statement

Reference

Tbcd¹ has adult antennal lobe projection neuron DL1 adPN | somatic clone | cell autonomous phenotype, non-suppressible by ds^UAS.cTa/Scer\GAL4^GH146

(Okumura et al., 2015)

Tbcd¹ has adult antennal lobe projection neuron DL1 adPN | somatic clone | cell autonomous phenotype, non-suppressible by CG11723^UAS.cOa/Scer\GAL4^GH146

(Okumura et al., 2015)

Tbcd¹ has adult antennal lobe projection neuron DL1 adPN | somatic clone | cell autonomous phenotype, non-suppressible by CG4259^UAS.cOa/Scer\GAL4^GH146

(Okumura et al., 2015)

NOT Enhancer of

Statement

Reference

TBCD[+]/Tbcd¹ is a non-enhancer of adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone phenotype of Dscam1⁰⁵⁵¹⁸

(Okumura et al., 2015)

Suppressor of

Statement

Reference

TBCD[+]/Tbcd¹ is a suppressor of adult mushroom body alpha-lobe phenotype of Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107

(Okumura et al., 2015)

TBCD[+]/Tbcd¹ is a suppressor of adult mushroom body beta-lobe phenotype of Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^ey-OK107

(Okumura et al., 2015)

Other

Statement

Reference

Hsap\TBCD^M387R.UAS, Scer\GAL4^GH146, Tbcd¹ has adult antennal lobe projection neuron | somatic clone phenotype

(Miyake et al., 2016)

Hsap\TBCD^R772C.UAS, Scer\GAL4^GH146, Tbcd¹ has adult antennal lobe projection neuron | somatic clone phenotype

(Miyake et al., 2016)

Hsap\TBCD^R772C.UAS, Scer\GAL4^GH146, Tbcd¹ has axon | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^R772C.UAS, Scer\GAL4^GH146, Tbcd¹ has dendrite | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^R772C.UAS, Scer\GAL4^GH146, Tbcd¹ has axon collateral | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^M387R.UAS, Scer\GAL4^GH146, Tbcd¹ has axon | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^M387R.UAS, Scer\GAL4^GH146, Tbcd¹ has dendrite | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^M387R.UAS, Scer\GAL4^GH146, Tbcd¹ has axon collateral | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^Y760stop.UAS, Scer\GAL4^GH146, Tbcd¹ has adult antennal lobe projection neuron | somatic clone phenotype

(Miyake et al., 2016)

Hsap\TBCD^Y760stop.UAS, Scer\GAL4^GH146, Tbcd¹ has axon | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^Y760stop.UAS, Scer\GAL4^GH146, Tbcd¹ has dendrite | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Hsap\TBCD^Y760stop.UAS, Scer\GAL4^GH146, Tbcd¹ has axon collateral | somatic clone | adult stage phenotype

(Miyake et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ds^Scer\UAS.cTa under the control of Scer\GAL4^GH146 does not rescue the defects seen in TBCD¹ mutant DL1 projection neuron clones.

Expression of Tbce^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^GH146 enhances the frequency of dendrite defects seen in TBCD¹ mutant DL1 projection neuron clones.

Dscam1⁰⁵⁵¹⁸ DL1 projection neuron mutant clones generated in a TBCD¹/+ background have an increased number of dendrites that spill over to the outside of the antennal lobe compared to wild type clones in a TBCD¹/+ mutant background. The loss of the dorsal branch seen in Dscam1⁰⁵⁵¹⁸ mutant clones alone is not enhanced in a TBCD¹ mutant background.

Expression of CG11723^Scer\UAS.cOa under the control of Scer\GAL4^GH146 does not suppress the defects seen in TBCD¹ mutant DL1 projection neuron clones.

Expression of CG4259^Scer\UAS.cOa under the control of Scer\GAL4^GH146 does not suppress the defects seen in TBCD¹ mutant DL1 projection neuron clones.

A TBCD¹/+ mutant background partially suppresses the loss of mushroom body α and β lobes seen in flies expressing Dscam1^{exon17.2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^ey-OK107.

(Okumura et al., 2015)

Xenogenetic Interactions

Statement

Reference

The axonal as well as dendritic morphological defects observed in TBCD¹ mutant olfactory projection neurons MARCM clones can be partially rescued by expression of Hsap\TBCD^Scer\UAS.cMa under the control of Scer\GAL4^GH146, whereas the expression of any of the following Hsap\TBCD^{M387R.Scer\UAS}, Hsap\TBCD^{Y760stop.Scer\UAS} or Hsap\TBCD^{R772C.Scer\UAS} has significantly reduced rescue ability.

(Miyake et al., 2016)

Complementation and Rescue Data

Complements

Glyp^MI00957

(Okumura et al., 2015)

AIF^GE14994

(Okumura et al., 2015)

AIF^e04281

(Okumura et al., 2015)

aop^03953a

(Okumura et al., 2015)

Rescued by