UAS regulatory sequences drive expression of a full-length Six4 open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.
The expression Six4UAS.ORF.GW.Tag:HA under the control of Scer\GAL4pnt-14-94 leads to a decrease in the number of type II neuroblast lineages with mature intermediate neural progenitors, as well as a decrease in the number of mature intermediate neural progenitor per type II neuroblast lineage in the larval brain.
The expression of Six4Scer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4109-30, in combination with gal80[ts] to restrict expression to adulthood, leads to an increase in the number of basal stalk cells, associated with defects in the formation of basal stalk, as compared to controls; clonal follicle stem cells expressing Six4Scer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4tub.PU do not present differences in cell competition capacity, as seen by the absence of significant differences in clonal expansion or extinction as compared to wild-type control clones.
Expression of Six4Scer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in male flies with severe wing size and patterning defects.
Expression of Six4Scer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Bx-MS1096 results in female flies with almost complete wing loss.
Scer\GAL4pnt-14-94, Six4UAS.ORF.GW.Tag:HA has abnormal neuroanatomy | larval stage phenotype, suppressible by pros[+]/pros17
Scer\GAL4pnt-14-94, Six4UAS.ORF.GW.Tag:HA has decreased cell number | larval stage phenotype, suppressible by pros[+]/pros17
Scer\GAL4insc-Mz1407/Six4UAS.ORF.GW.Tag:HA is a suppressor of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4insc-Mz1407, pntUAS.P1.cZa
Scer\GAL4insc-Mz1407/Six4UAS.ORF.GW.Tag:HA is a suppressor of increased cell number | larval stage phenotype of Scer\GAL4insc-Mz1407, pntUAS.P1.cZa
Six4UAS.ORF.GW.Tag:HA/Scer\GAL4GMR9D11 is a suppressor of increased cell number | larval stage phenotype of Scer\GAL4GMR9D11, ermJF02342
Six4UAS.ORF.GW.Tag:HA/Scer\GAL4GMR9D11 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4GMR9D11, ermJF02342
Scer\GAL4pnt-14-94, Six4UAS.ORF.GW.Tag:HA has embryonic/larval brain | larval stage phenotype, suppressible by pros[+]/pros17
Scer\GAL4pnt-14-94, Six4UAS.ORF.GW.Tag:HA has type II neuroblast | increased number | larval stage phenotype, suppressible by pros[+]/pros17
Scer\GAL4pnt-14-94, Six4UAS.ORF.GW.Tag:HA has intermediate neuronal progenitor | larval stage | decreased number phenotype, suppressible by pros[+]/pros17
Scer\GAL4insc-Mz1407/Six4UAS.ORF.GW.Tag:HA is a suppressor of larval ventral nerve cord phenotype of Scer\GAL4insc-Mz1407, pntUAS.P1.cZa
Scer\GAL4insc-Mz1407/Six4UAS.ORF.GW.Tag:HA is a suppressor of type I neuroblast | larval stage phenotype of Scer\GAL4insc-Mz1407, pntUAS.P1.cZa
Six4UAS.ORF.GW.Tag:HA/Scer\GAL4GMR9D11 is a suppressor | partially of type II neuroblast | increased number | larval stage phenotype of Scer\GAL4GMR9D11, ermJF02342
Six4UAS.ORF.GW.Tag:HA/Scer\GAL4GMR9D11 is a suppressor | partially of embryonic/larval brain | larval stage phenotype of Scer\GAL4GMR9D11, ermJF02342
Scer\GAL4insc-Mz1407/Six4UAS.ORF.GW.Tag:HA is a suppressor of intermediate neuronal progenitor | increased number | larval stage phenotype of Scer\GAL4insc-Mz1407, pntUAS.P1.cZa