FlyBase Allele Report: Dmel\Nrg[180ΔFIGQY]

General Information

Symbol

Dmel\Nrg^180ΔFIGQY

Species

D. melanogaster

Name

FlyBase ID

FBal0291694

Feature type

allele

Associated gene

Dmel\Nrg

Associated Insertion(s)

Carried in Construct

P{Nrg.180ΔFIGQY}

Also Known As

P[nrg180ΔFIGQY]

Key Links

Transgenic product class

inframe_deletion

(Enneking et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Enneking et al., 2013)

Mutagen

in vitro construct

(Enneking et al., 2013)

Progenitor genotype

Nrg^+t25

(Enneking et al., 2013)

Carried in construct

P{Nrg.180ΔFIGQY}

Cytology

Description

The Nrg180 Ankyrin-binding domain in Nrg^+t25 has been deleted.

(Enneking et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

Nrg

Encoded product / tool

Nrg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Nrg^l4

(Enneking et al., 2013, Kudumala et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Nrg^l4

(Enneking et al., 2013)

abnormal neurophysiology | adult stage, with Nrg^l4

(Enneking et al., 2013, Kudumala et al., 2013)

Phenotype Manifest In

adult neuromuscular junction, with Nrg^l4

(Kudumala et al., 2013)

embryonic/larval neuromuscular junction | third instar larval stage, with Nrg^l4

(Enneking et al., 2013)

giant fiber neuron | adult stage, with Nrg^l4

(Enneking et al., 2013, Kudumala et al., 2013)

NMJ bouton | third instar larval stage, with Nrg^l4

(Enneking et al., 2013)

Detailed Description

Statement

Reference

Expression of Nrg^180ΔFIGQY in a Nrg^l4 mutant background does not result in a significant increase in synaptic retraction in third instar larvae compared to controls. NMJ length and bouton number are increased compared to controls. The bouton area is significantly reduced compared to controls.

Flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background are unable to establish a normal function of the Giant Fibre (GF)-Trochanteral Muscle (TTMn) circuit; there are significant increases in average response latencies and a decrease in following frequencies after a train of stimulations at 100Hz when compared to wild type. Morphological defects are seen at GF terminals. The GFs are present at the synaptic target area, however large areas of the synaptic terminals are either thinner or swollen and often contain large vacuole-like structures. Synaptic gap junctions are still present in animals that do not show any functional response.

(Enneking et al., 2013)

The synaptic Giant Fibre (GF) to Trochanteral Muscle (TTMn) connection is severely weakened in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background. The GF synapse is unable to follow multiple stimuli given at 100 Hz in a 1:1 ratio and Response Latency is increased. Synaptic terminals are morphologically defective and reduced in size.

(Kudumala et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, suppressible by Hsap\L1CAM^RSLE-.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, suppressible by Hsap\L1CAM^RSLE-.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, suppressible by Hsap\L1CAM^L120V.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, suppressible by Hsap\L1CAM^L120V.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, suppressible by Hsap\L1CAM^H38.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, suppressible by Hsap\L1CAM^H38.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, suppressible | partially by Hsap\L1CAM^C264Y.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Hsap\L1CAM^C264Y.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, suppressible by Hsap\L1CAM^4A.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, suppressible by Hsap\L1CAM^4A.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

NOT suppressed by

Statement

Reference

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Hsap\L1CAM^H1.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Hsap\L1CAM^H1.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Hsap\L1CAM^H210Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Hsap\L1CAM^H210Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Hsap\L1CAM^R184Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Hsap\L1CAM^R184Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neurophysiology | adult stage phenotype, non-suppressible by Hsap\L1CAM^UAS.1180/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Hsap\L1CAM^UAS.1180/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, suppressible by Hsap\L1CAM^RSLE-.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction phenotype, suppressible by Hsap\L1CAM^RSLE-.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, suppressible by Hsap\L1CAM^L120V.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction | adult stage phenotype, suppressible by Hsap\L1CAM^L120V.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, suppressible by Hsap\L1CAM^H38.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction | adult stage phenotype, suppressible by Hsap\L1CAM^H38.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, suppressible | partially by Hsap\L1CAM^C264Y.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction | adult stage phenotype, suppressible | partially by Hsap\L1CAM^C264Y.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, suppressible by Hsap\L1CAM^4A.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction | adult stage phenotype, suppressible by Hsap\L1CAM^4A.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

NOT suppressed by

Statement

Reference

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, non-suppressible by Hsap\L1CAM^UAS.1180/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction phenotype, non-suppressible by Hsap\L1CAM^UAS.1180/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, non-suppressible by Hsap\L1CAM^H1.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction | adult stage phenotype, non-suppressible by Hsap\L1CAM^H1.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, non-suppressible by Hsap\L1CAM^H210Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction | adult stage phenotype, non-suppressible by Hsap\L1CAM^H210Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has giant fiber neuron | adult stage phenotype, non-suppressible by Hsap\L1CAM^R184Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Nrg^180ΔFIGQY, Nrg^l4 has adult neuromuscular junction | adult stage phenotype, non-suppressible by Hsap\L1CAM^R184Q.UAS/Scer\GAL4^shot-OK307

(Kudumala et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\L1CAM^{RSLE-.Scer\UAS} under the control of Scer\GAL4^OK307 suppresses the Giant Fibre (GF)-Trochanteral Muscle (TTM) synaptic defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background; there is an increase in following frequencies after a train of stimulations at 100Hz and response latency is decreased. All flies observed show TTM responses upon GF stimulation. The morphology of the synaptic terminals is restored.

Expression of Hsap\L1CAM^{Scer\UAS.1180} under the control of Scer\GAL4^OK307 does not suppress the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background.

Expression of Hsap\L1CAM^H1.Scer\UAS under the control of Scer\GAL4^OK307 does not suppress the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background.

Expression of Hsap\L1CAM^{H210Q.Scer\UAS} under the control of Scer\GAL4^OK307 does not suppress the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background.

Expression of Hsap\L1CAM^{R184Q.Scer\UAS} under the control of Scer\GAL4^OK307 does not suppress the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background.

Expression of Hsap\L1CAM^{L120V.Scer\UAS} under the control of Scer\GAL4^OK307 significantly suppresses the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background.

Expression of Hsap\L1CAM^H38.Scer\UAS under the control of Scer\GAL4^OK307 significantly suppresses the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background.

Expression of Hsap\L1CAM^{C264Y.Scer\UAS} under the control of Scer\GAL4^OK307 significantly suppresses the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background. Half of the tested GF to TTM connections are functionally normal, while the other half are functionally impaired.

Expression of Hsap\L1CAM^4A.Scer\UAS under the control of Scer\GAL4^OK307 significantly suppresses the Giant Fibre (GF)-Trochanteral Muscle (TTM) synapse functional and morphological defects seen in flies expressing Nrg^180ΔFIGQY in a Nrg^l4 mutant background.

(Kudumala et al., 2013)

Complementation and Rescue Data

Rescues

Nrg^180ΔFIGQY rescues Nrg^l4

(Zhang et al., 2014)

Nrg^UAS.180.cEa, Nrg^180ΔFIGQY, Scer\GAL4^shot-OK307 rescues Nrg^l4

(Enneking et al., 2013)

Nrg^180ΔFIGQY rescues Nrg^l7

(Enneking et al., 2013)

Partially rescues

Nrg^180ΔFIGQY partially rescues Nrg^l4

(Enneking et al., 2013)

Comments

Expression of Nrg^180ΔFIGQY fully rescues Nrg^l4 mutant embryonic lethality. The axonal and NMJ defects seen in Nrg^l4 mutant larval motorneuron clones are only partially rescued and similar rates of innervation are seen for all four major classes of motorneurons (SNc/d, SNb, SNa and ISN) as controls.

Expression of Nrg^180ΔFIGQY fully rescues Nrg^l7 mutant embryonic lethality.

Expression of Nrg^{Scer\UAS.180.cEa} under the control of Scer\GAL4^OK307 significantly rescues the electrophysiological and morphological defects seen at the adult Giant Fibre (GF)-Trochanteral Muscle (TTMn) synapse when Nrg^180ΔFIGQY is expressed in a Nrg^l4 mutant background. Rescue is also seen when Nrg^{Scer\UAS.180.cEa} is expressed presynaptically (Scer\GAL4^c17) or postsynaptically (Scer\GAL4^{shakB.lethal.4.1}), but not when Scer\GAL4^c42.2 is expressed presynaptically only after the connection between the GF and the TTMn has been established.

(Enneking et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nrg^180ΔFIGQY

Name Synonyms

Secondary FlyBase IDs

References (6)

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