FlyBase Allele Report: Dmel\DnaJ-1[UAS.cKb]

General Information

Symbol

Dmel\DnaJ-1^UAS.cKb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Kuo

FlyBase ID

FBal0291059

Feature type

allele

Associated gene

Dmel\DnaJ-1

Associated Insertion(s)

Carried in Construct

P{UAS-DNaJ-1.Kb}

Also Known As

UAS-dnaJ-1

Key Links

Transgenic product class

cDNA

(Kuo et al., 2013)

wild_type

(Kuo et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kuo et al., 2013)

wild_type

(Kuo et al., 2013)

Mutagen

in vitro construct

(Kuo et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-DNaJ-1.Kb}

Cytology

Description

UAS regulatory sequences drive expression of a DnaJ-1 cDNA (EST clone SD08787).

(Kuo et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

DnaJ-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}, Hsp110^UAS.cKa

(Kuo et al., 2013)

ameliorates Huntington's disease

modeled by Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

modeled by Hsap\HTT^GMR.Q120, Hsp110^UAS.cKa

(Kuo et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, suppressible by Hsp83^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

NOT suppressed by

Statement

Reference

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsp60A^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsp68^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsc70-1^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsc70-2^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsc70-3^UAS.cEa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsc70-5^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsp110^K68S.UAS, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has visible | adult stage phenotype, non-suppressible by Hsp110^KD.UAS, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

Suppressor of

Statement

Reference

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Scer\GAL4^GMR.PU, DnaJ-1^UAS.cKb is a suppressor | partially of visible | adult stage phenotype of Df(3R)Hsp70A, Df(3R)Hsp70B, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

NOT Suppressor of

Statement

Reference

Hsp110^HMS01080/DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, suppressible by Hsp83^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, suppressible by Hsp83^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Hsap\HTT^GMR.Q120, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Hsp110^UAS.cKa, Scer\GAL4^GMR.PF

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Hsap\HTT^GMR.Q120, Scer\GAL4^GMR.PF has rhabdomere phenotype, suppressible by Hsp110^UAS.cKa, Scer\GAL4^GMR.PF

(Kuo et al., 2013)

NOT suppressed by

Statement

Reference

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsp60A^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsp68^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsc70-2^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsc70-3^UAS.cEa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsc70-5^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsp110^K68S.UAS, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsp110^K68S.UAS, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsp110^KD.UAS, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsp110^KD.UAS, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by mrj^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by mrj^UAS.Tag:FLAG, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsc70-1^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsc70-2^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsc70-3^UAS.cEa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsc70-4^UAS.cEa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has eye phenotype, non-suppressible by Hsc70-5^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsp60A^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsp68^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA} has photoreceptor phenotype, non-suppressible by Hsc70-1^UAS.cKa, Scer\GAL4^GMR.PU

(Kuo et al., 2013)

Suppressor of

Statement

Reference

DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Scer\GAL4^GMR.PU, DnaJ-1^UAS.cKb is a suppressor | partially of eye phenotype of Df(3R)Hsp70A, Df(3R)Hsp70B, Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

DnaJ-1^UAS.cKb/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

DnaJ-1^UAS.cKb/Scer\GAL4^GMR.PF is a suppressor of rhabdomere phenotype of Hsap\HTT^GMR.Q120

(Kuo et al., 2013)

NOT Suppressor of

Statement

Reference

Hsp110^HMS01080/DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Hsp110^HMS01080/DnaJ-1^UAS.cKb, Scer\GAL4^GMR.PU is a non-suppressor of photoreceptor phenotype of Scer\GAL4^GMR.PU, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Kuo et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of DnaJ-1^Scer\UAS.cKb suppresses the eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PU. Young animals have spotted pigment loss in the retina, indicating the degeneration of retinal cells, and this phenotype is more visible in aged animals. The photoreceptor loss is partially suppressed; ommatidia are observed that have small and missing rhabdomeres. The phenotype is still suppressed in a Df(3R)Hsp70A Df(3R)Hsp70B mutant background.

Expression of Hsc70Cb^Scer\UAS.cKa suppresses the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU. No pigmentation loss is seen in either young or aged animals and at one day old all seven photoreceptors with normal sized rhabdomeres are detected.

Expression of Hsp83^Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70-5^Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70-4^Scer\UAS.cEa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70-3^Scer\UAS.cEa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70-2^Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70-1^Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsp68^Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsp60^Scer\UAS.cKa does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

In 10 day old animals, severe neuronal degeneration marked by vesicle accumulation, loss of rhabdomeres, and invasion of pigment granules is detected in flies expressing Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70Cb^KD.Scer\UAS does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70Cb^{K68S.Scer\UAS} does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} and DnaJ-1^Scer\UAS.cKb are co-expressed under the control of Scer\GAL4^GMR.PU.

Co-expression of Hsc70Cb^HMS01080 and DnaJ-1^Scer\UAS.cKb does not suppress the external eye degeneration phenotype seen when Zzzz\CAG^{127Q.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PU. Complete neuronal degeneration is observed in these flies.

Expression of DnaJ-1^Scer\UAS.cKb under the control of Scer\GAL4^GMR.PF slows the neural eye degeneration caused by expression of Hsap\HTT^GMR.Q120. Some degeneration is observed at 10 days, and complete photoreceptor neuron loss is observed at 15 days.

Hsc70Cb^Scer\UAS.cKa suppresses the neural eye degeneration caused by seen when Hsap\HTT^GMR.Q120 and DnaJ-1^Scer\UAS.cKb are expressed under the control of Scer\GAL4^GMR.PF. Essentially normal morphology is seen in the retinas of these flies at 10 days, although some degeneration is seen at 15 days.

(Kuo et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DnaJ-1^Scer\UAS.cKb

DnaJ-1^UAS.cKb

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Kuo

Secondary FlyBase IDs

References (2)

Report Sections

Open Close