FB2024_03 , released June 25, 2024
Allele: Dmel\AxnαTub84B.Tag:FLAG
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General Information
Symbol
Dmel\AxnαTub84B.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0285524
Feature type
allele
Associated gene
Dmel\Axn
Associated Insertion(s)
Carried in Construct
P{αTub84B(-FRT)Axn.FLAG}
Key Links
Transgenic product class
cDNA
(Peterson-Nedry et al., 2008)
wild_type
(Peterson-Nedry et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Peterson-Nedry et al., 2008)
wild_type
(Peterson-Nedry et al., 2008)
Mutagen
FLPase
(Peterson-Nedry et al., 2008)
Progenitor genotype
AxnFRT.αTub84B.Tag:FLAG
(Peterson-Nedry et al., 2008)
Carried in construct
P{αTub84B(-FRT)Axn.FLAG}
Cytology
Description

Excision of the FRT-w cassette from progenitor AxnFRT.αTub84B.Tag:FLAG allows the αTub84B promoter to drive expression of full-length Axn fused with N-terminal 6xTag:FLAG.

(Peterson-Nedry et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
Axn
Tagged with
Tag:FLAG
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Transgenic flies co-expressing Scer\GAL4ptc-559.1>AxnScer\UAS.T:Zzzz\FLAG and AxnαTub84B.T:Zzzz\FLAG in addition to endogenous wild-type Axn show no visible defects in the bristle pattern or at the wing margin.

      Transgenic flies expressing maternally deposited AxnαTub84B.T:Zzzz\FLAG in addition to endogenous Axn show no visible defects in adult structures including the wing, although AxnαTub84B.T:Zzzz\FLAG-expressing embryos show about 20% lower survival compared with wild-type animals.

      The hatch rate of embryos expressing zygotic AxnαTub84B.T:Zzzz\FLAG is not significantly affected. The majority of embryos complete development and reach adulthood.

      (Peterson-Nedry et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      AxnαTub84B.Tag:FLAG rescues AxnE77

      (Peterson-Nedry et al., 2008)

      AxnαTub84B.Tag:FLAG rescues AxnS044230

      (Peterson-Nedry et al., 2008)
      Comments

      Maternal expression of AxnαTub84B.T:Zzzz\FLAG in AxnS044230 (maternal-/zygotic-) embryos restores the wild-type pattern of denticle belts. No ectopic denticles are present. Maternal expression of AxnS044230 allows AxnS044230 (maternal-/zygotic-) embryos to hatch.

      Expression of AxnαTub84B.T:Zzzz\FLAG rescues both the viability and fertility of AxnE77. The rescued flies display normal wing margin.

      (Peterson-Nedry et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AxnαTub84B.T:Zzzz\FLAG
      AxnαTub84B.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (4)