FB2024_03 , released June 25, 2024
Allele: Dmel\bsh1
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General Information
Symbol
Dmel\bsh1
Species
D. melanogaster
Name
FlyBase ID
FBal0284935
Feature type
allele
Associated gene
Dmel\bsh
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Hasegawa et al., 2013)
Mutagen
Delta2-3 transposase
(Hasegawa et al., 2013)
Progenitor genotype
P{EP}bshG3075
(Hasegawa et al., 2013)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a 3062bp deletion (coordinates 2L:19766024..19769085 , release 5 genome) which extends from 156bp upstream of the first bsh start codon to 336bp downstream of the bsh stop codon.

(Hasegawa et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:19,766,024..19,769,085 [-]
Comment:

A 3062 bp deletion resulting from the imprecise excision of P{EP}bshG3075 maps to R5 coordinates 2L:19766024..19769085 .

(Hasegawa et al., 2013)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In bsh1/In bsh2 mutants, there is strong disorganization of the columnar structures in the larval and pupal medulla.

      (Trush et al., 2019)

      MARCM neuron clones in the medulla that would normally express bsh (labelled using Scer\GAL4bsh.PH) but which are homozygous for bsh1 are transformed into transmedullary neuron (Tm)-type neurons in 89.6% of cases (bsh-expressing neurons normally develop into Mi1 medulla intrinsic neurons in wild-type animals).

      MARCM neuron clones in the lamina that would normally express bsh (labelled using Scer\GAL4bsh.PH) but which are homozygous for bsh1 develop into L3-like neurons in 91.7% of cases (bsh is normally expressed in L4 and L5 lamina neurons in wild-type).

      (Hasegawa et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      bsh1 is partially rescued by bshUAS.PA/Scer\GAL4bsh.PH

      (Hasegawa et al., 2013)

      bsh1 is partially rescued by Scer\GAL4bsh.PH/bshUAS.PB

      (Hasegawa et al., 2013)
      Comments

      Expression of bshScer\UAS.PB under the control of Scer\GAL4bsh.PH partially rescues the transformation of Mi1 medulla intrinsic neurons into transmedullary neuron (Tm)-type neurons seen in bsh1 MARCM neuron clones. The projections of the rescued neurons are restricted to the medulla and arborisations are formed at the M1, M5 and M9-M10 layers, but the size and shape of the arborisations is not identical to wild-type Mi1 neurons.

      Expression of bshScer\UAS.PA under the control of Scer\GAL4bsh.PH mildly rescues the transformation of Mi1 medulla intrinsic neurons into transmedullary neuron (Tm)-type neurons seen in bsh1 MARCM neuron clones.

      Expression of bshScer\UAS.PB under the control of Scer\GAL4bsh.PH partially rescues the formation of ectopic L3-like lamina neurons seen in bsh1 MARCM neuron clones.

      (Hasegawa et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      bsh1
      (Trush et al., 2019, Hasegawa et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)