FB2024_03 , released June 25, 2024
Allele: Dmel\CragC
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General Information
Symbol
Dmel\CragC
Species
D. melanogaster
Name
FlyBase ID
FBal0283008
Feature type
allele
Associated gene
Dmel\Crag
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Xiong et al., 2012)
Mutagen
ethyl methanesulfonate
(Graves et al., 2020, Xiong et al., 2012)
Progenitor genotype
Cytology
Description

Amino acid replacement: R798term.

(Graves et al., 2020, Xiong et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
X:8,596,513..8,596,513 [+]
Nucleotide change:

C8596513T

Amino acid change:

R798term | Crag-PA; R798term | Crag-PB; R798term | Crag-PD

Reported amino acid change:

R798term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Xiong et al., 2012)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Rhabdomere morphology and photoreceptor axon targeting is normal in 1 day old flies containing homozygous clones in the eye.

      Electroretinogram (ERG) responses of flies containing homozygous clones in the eye are normal for 1 and 14 day old flies kept in constant darkness. However, if the flies are reared under 12 hour light/12 hour dark conditions, both the amplitude of the ERG and the size of the "on" and "off" transients become smaller with age. If the flies are kept under constant light conditions, the ERG amplitude is severely affected, showing a 90% reduction after only 5 days. After 2 week in constant light conditions, the rhabdomeres are severely damaged and the terminals of the photoreceptor cells are disrupted.

      After 5 days under 12 hour light/12 hour dark conditions, mutant photoreceptors show a massive accumulation of vesicles in the cytosol.

      Mutant photoreceptors show an enlargement of the trans-Golgi network compared to wild type after 6 hours of blue light exposure and 18 hours of dark recovery.

      (Xiong et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Other
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      CragC has rhabdomere | somatic clone | progressive | conditional phenotype, suppressible | partially by Scer\GAL4ey.PH/Rab11Q70L.UASp.YFP

      (Xiong et al., 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The reduction in amplitude and in the size of the "on" and "off" transients of the electroretinogram that is seen in 3 week old flies containing homozygous CragC clones in the eye when they are raised under 12 hour light/12 hour dark conditions is partially suppressed by expression of Rab11Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP under the control of Scer\GAL4ey.PH. Rhabdomere morphology is also better preserved in these animals compared to flies containing homozygous CragC clones in the eye in an otherwise wild-type background.

      (Xiong et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      CragCJ101

      (Xiong et al., 2012)

      Cragf07899

      (Xiong et al., 2012)
      Rescued by

      CragC is rescued by Crag+t12.4

      (Xiong et al., 2012)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      CragC
      (Graves et al., 2020, Xiong et al., 2012)
      XE10C
      (Xiong et al., 2012)
      XE10C
      (Graves et al., 2020)
      XIYF011
      (Graves et al., 2020)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)