The complex mutation present in the C-terminal section of Dsim\Lhr2.Tag:HA has replaced by 10 residues of wild-type Dsim\Lhr sequence.
Hybrid Hmr1 males (progeny of Hmr1 Dsim\Lhr2+Cter.T:Ivir\HA1 D. melanogaster females mated to D. mauritiana males) carrying Dsim\Lhr2+Cter.T:Ivir\HA1 are essentially inviable at room temperature and show strongly reduced viability (compared to hybrid Hmr1 males not carrying Dsim\Lhr2+Cter.T:Ivir\HA1) at 18[o]C.
Dsim\Lhr2+Cter.T:Ivir\HA1 fully complements Dsim\Lhr1, which is observed as hybrid male inviability in the progeny of a cross of D. melanogaster females carrying Dsim\Lhr2+Cter.T:Ivir\HA1 to D. simulans Dsim\Lhr1 males.