FB2024_03 , released June 25, 2024
Allele: Dsim\Lhr2.Tag:HA
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General Information
Symbol
Dsim\Lhr2.Tag:HA
Species
D. simulans
Name
FlyBase ID
FBal0280197
Feature type
allele
Associated gene
Dsim\Lhr
Associated Insertion(s)
Carried in Construct
M{sim-Lhr2-HA}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Maheshwari and Barbash, 2012)
Progenitor genotype
Dsim\Lhr2
(Maheshwari and Barbash, 2012)
Dsim\Lhr+tMa
(Maheshwari and Barbash, 2012)
Carried in construct
M{sim-Lhr2-HA}
Cytology
Description

The wild-type Dsim\Lhr coding sequence present in Dsim\Lhr+tMa has been replaced with the mutant Dsim\Lhr2 coding sequence. Three copies of the Tag:HA tag have been inserted at the C-terminal end of the mutant coding region.

(Maheshwari and Barbash, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
Dsim\Lhr
Encoded product / tool
Dsim\Lhr
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Hybrid Hmr1 males (progeny of Hmr1 Dsim\Lhr2.T:Ivir\HA1 D. melanogaster females mated to D. mauritiana males) carrying Dsim\Lhr2.T:Ivir\HA1 are fully viable at 18[o]C and have substantially higher viability (138.8-144.3%) at room temperature compared to hybrid Hmr1 male siblings not carrying Dsim\Lhr2.T:Ivir\HA1.

      (Maheshwari and Barbash, 2012)
      Complementation and Rescue Data
      Partially rescues

      Dsim\Lhr2.Tag:HA partially rescues Dsim\Lhr1

      (Maheshwari and Barbash, 2012)
      Comments

      Dsim\Lhr2.T:Ivir\HA1 partially complements Dsim\Lhr1, which is observed as 35-40% hybrid male viability in the progeny of a cross of D. melanogaster females carrying Dsim\Lhr2.T:Ivir\HA1 to D. simulans Dsim\Lhr1 males.

      (Maheshwari and Barbash, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dsim\Lhr2.T:Ivir\HA1
      Dsim\Lhr2.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)