FlyBase Allele Report: Dmel\Med[K738R.UASp]

General Information

Symbol

Dmel\Med^K738R.UASp

Species

D. melanogaster

Name

FlyBase ID

FBal0280158

Feature type

allele

Associated gene

Dmel\Med

Associated Insertion(s)

Carried in Construct

P{UASp-Med.K738R}

Key Links

Transgenic product class

missense_variant

(Stinchfield et al., 2012)

polypeptide_post_translational_processing_variant

(Stinchfield et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Stinchfield et al., 2012)

polypeptide_post_translational_processing_variant

(Stinchfield et al., 2012)

Mutagen

in vitro construct

(Stinchfield et al., 2012)

Progenitor genotype

Carried in construct

P{UASp-Med.K738R}

Cytology

Description

UASp regulatory sequences drive expression of a mutated form of Med, which contains the amino acid replacement K738R (the mutation is designed to produce a non-ubiquitylatable form of Med).

(Stinchfield et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Med

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Med^K738R.UASp is a suppressor of partially lethal - majority die | maternal effect phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

Med^K738R.UASp is a suppressor of partially lethal - majority die | maternal effect phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}/Med^K738R.UASp is a suppressor | partially of lethal | maternal effect phenotype of dpp^hr27, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

Med^K738R.UASp is a suppressor | partially of lethal | maternal effect phenotype of dpp^hr27, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}/Med^K738R.UASp is a suppressor of lethal | dominant | maternal effect | embryonic stage phenotype of dpp^hr4/dpp[+], faf^FO8

(Stinchfield et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/Med^K738R.UASp is a suppressor | partially of embryo phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

Med^K738R.UASp is a suppressor | partially of embryo phenotype of dpp^hr4, lolal[+]/lolal¹¹²²

(Quijano et al., 2016)

Med^K738R.UASp is a suppressor | partially of embryonic/first instar larval cuticle | maternal effect phenotype of dpp^hr4/dpp[+], faf^FO8

(Stinchfield et al., 2012)

Scer\GAL4^{VP16.nanos.UTR}/Med^K738R.UASp is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of dpp^hr4/dpp[+], faf^FO8

(Stinchfield et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The maternal expression of Med^{K738R.Scer\UAS.P\T}, either under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} or in the absence of the Gal4 driver, fully rescues the near lethality and partially rescues the embryo ventralization observed in the progeny from dpp^hr4/+ fathers and lolal¹¹²²/+ mothers; this maternal expression, either under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} or in the absence of the Gal4 driver, also partially rescues the lethality of the progeny from dpp^hr27/+ fathers and lolal¹¹²²/+ mothers.

(Quijano et al., 2016)

The survival of the progeny of faf^FO8/+ females mated to dpp^hr4/+ males is increased 4.2-fold by basal expression of Med^{K738R.Scer\UAS.P\T} (in the absence of a Scer\GAL4 driver) in the females. Expression of Med^{K738R.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the females increases survival of the progeny to approximately 80% in both cases.

The ventralised cuticle phenotype of embryos derived from faf^FO8/+ females mated to dpp^hr4/+ males is rescued in a proportion of the embryos by basal expression of Med^{K738R.Scer\UAS.P\T} (in the absence of a Scer\GAL4 driver) in the females. Expression of Med^{K738R.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the females effectively rescues the ventralised cuticle phenotype of the embryos.

(Stinchfield et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The survival of the progeny of Med⁸/+ females mated to dpp^hr4/+ males is increased 3.6-fold by basal expression of Med^{K738R.Scer\UAS.P\T} (in the absence of a Scer\GAL4 driver) in the females. Expression of Med^{K738R.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the females fully rescues the lethality of the embryos.

(Stinchfield et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Med^{K738R.Scer\UAS.P\T}

Med^K738R.UASp

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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