FlyBase Allele Report: Dmel\Marf[RNAi.cUa.UAS]

General Information

Symbol

Dmel\Marf^RNAi.cUa.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0279441

Feature type

allele

Associated gene

Dmel\Marf

Associated Insertion(s)

Carried in Construct

P{UAS-Marf.RNAi.U}

Also Known As

Marf RNAi, UAS-mfn RNAi

Key Links

Transgenic product class

RNAi_reagent

(Dorn et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dorn et al., 2011)

Mutagen

in vitro construct

(Dorn et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-Marf.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of a Marf RNAi construct.

(Dorn et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Marf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Charcot-Marie-Tooth disease type 2

CEA

(Kandul et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cardiomyopathy

modeled by park^HMS01651

(Bhandari et al., 2014)

modeled by park^HMS01800

(Bhandari et al., 2014)

ameliorates Friedreich ataxia 1

modeled by fh^RNAi.UAS.cAa

(Edenharter et al., 2018)

ameliorates Parkinson's disease

modeled by Miro^UAS.Tag:MYC

(Lee et al., 2018)

model of Charcot-Marie-Tooth disease type 2

is exacerbated by CG13551^UAS.cKa

(Kandul et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

lethal - all die before end of larval stage, with Scer\GAL4^Tub.PU

(Dorn et al., 2011)

some die during larval stage, with Scer\GAL4^Tub.PU

(Dorn et al., 2011)

viable, with Scer\GAL4^tin.CΔ4

(Dorn et al., 2011)

visible, with Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Phenotype Manifest In

adult heart, with Scer\GAL4^tin.CΔ4

(Bhandari et al., 2014, Eschenbacher et al., 2012, Dorn et al., 2011)

cardial cell, with Scer\GAL4^tin.CΔ4

(Bhandari et al., 2014)

cardial cell | adult stage, with Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012, Dorn et al., 2011)

eye, with Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

mitochondrion, with Scer\GAL4^tin.CΔ4

(Bhandari et al., 2014)

mitochondrion | adult stage, with Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012, Dorn et al., 2011)

ommatidium, with Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Detailed Description

Statement

Reference

Flies expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 show a dilated cardiomyopathy characterised by mitochondria with fewer genomes, increased reactive oxygen species (ROS) and greater depolarization. There is no reduction in mitochondrial DNA.

(Bhandari et al., 2014)

Expression of Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel results in an approximately 30% reduction in eye area and results in ommatidial disorganisation.

Flies expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 show a progressive reduction in climbing activity compared to controls as they age. Heart tube fractional shortening is significantly depressed in the mutant flies at 4 weeks of age.

Expression of Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 increases mitochondrial heterogeneity, induces mitochondrial fragmentation in adult cardiomyocytes and shifts the population distribution towards smaller mitochondria.

(Eschenbacher et al., 2012)

Adults expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 have normal longevity.

Mitochondria tend to cluster in aggregates that distort the normal myofibrillar architecture in cardiomyocytes of adults expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4. The mitochondria appear heterogeneous, with both mitochondrial fragments and enlarged mitochondria being seen. Mean mitochondrial size is decreased approximately 30% compared to wild type. T-tubules and sarcoplasmic reticulum appears morphologically normal in the mutant cardiomyocytes.

The heart tubes of adults expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 are dilated compared to wild type and show impaired shortening with no change in the beating rate.

(Dorn et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Marf^RNAi.cUa.UAS, Scer\GAL4^ey.3.5.Exel has visible phenotype, suppressible by Hsap\MFN2^UAS.cDa, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by Hsap\MFN2^UAS.cDa, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

NOT suppressed by

Statement

Reference

Hsap\HTT^Q93.ex1.UAS, Marf^RNAi.cUa.UAS, Scer\GAL4^elav.PU has abnormal neuroanatomy phenotype, non-suppressible by Pink1^UAS.Tag:HA, Scer\GAL4^elav.PU

(Khalil et al., 2015)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has abnormal locomotor behavior | adult stage | progressive phenotype, non-suppressible by Hsap\MFN2^M393I.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has abnormal locomotor behavior | adult stage | progressive phenotype, non-suppressible by Hsap\MFN2^R400Q.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by Hsap\MFN2^M393I.UAS, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^ey.3.5.Exel has visible phenotype, non-suppressible by Hsap\MFN2^R400Q.UAS, Scer\GAL4^ey.3.5.Exel

(Eschenbacher et al., 2012)

Suppressor of

Statement

Reference

Marf^RNAi.cUa.UAS, Scer\GAL4^VGlut-OK371 is a suppressor of abnormal size | larval stage phenotype of Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Marf^RNAi.cUa.UAS, Scer\GAL4^insc-Mz1407 is a suppressor | partially of abnormal neuroanatomy phenotype of N^GD144, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Marf^RNAi.cUa.UAS/Myc^UAS.cZa, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy phenotype of N^GD144, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Marf^RNAi.cUa.UAS, Scer\GAL4^Mhc.PW is a suppressor of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2

(Wu et al., 2013)

Marf^RNAi.cUa.UAS, Scer\GAL4^Mhc.PW is a suppressor of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A.UAS.L.Tag:FLAG}

(Wu et al., 2013)

NOT Suppressor of

Statement

Reference

Marf^RNAi.cUa.UAS, Scer\GAL4^elav.PU is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav.PU

(Khalil et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has adult heart phenotype, suppressible by park^HMS01651, Scer\GAL4^tin.CΔ4

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has cardial cell phenotype, suppressible by park^HMS01651, Scer\GAL4^tin.CΔ4

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has mitochondrion phenotype, suppressible | partially by park^HMS01651, Scer\GAL4^tin.CΔ4

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has adult heart phenotype, suppressible by Hsap\MFN2^UAS.cDa, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012, Dorn et al., 2011)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has mitochondrion | adult stage phenotype, suppressible by Hsap\MFN2^UAS.cDa, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has cardial cell | adult stage phenotype, suppressible by Hsap\MFN2^UAS.cDa, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has adult heart phenotype, suppressible by Sod1^UAS.cAa, Scer\GAL4^tin.CΔ4

(Dorn et al., 2011)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has mitochondrion | adult stage phenotype, suppressible | partially by Sod1^UAS.cAa, Scer\GAL4^tin.CΔ4

(Dorn et al., 2011)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has cardial cell | adult stage phenotype, suppressible | partially by Sod1^UAS.cAa, Scer\GAL4^tin.CΔ4

(Dorn et al., 2011)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has adult heart phenotype, suppressible by Hsap\MFN1^UAS.cDa, Scer\GAL4^tin.CΔ4

(Dorn et al., 2011)

NOT suppressed by

Statement

Reference

Hsap\HTT^Q93.ex1.UAS, Marf^RNAi.cUa.UAS, Scer\GAL4^elav.PU has eye photoreceptor cell phenotype, non-suppressible by Pink1^UAS.Tag:HA, Scer\GAL4^elav.PU

(Khalil et al., 2015)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has adult heart phenotype, non-suppressible by Hsap\MFN2^M393I.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has adult heart phenotype, non-suppressible by Hsap\MFN2^R400Q.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has mitochondrion | adult stage phenotype, non-suppressible by Hsap\MFN2^M393I.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has cardial cell | adult stage phenotype, non-suppressible by Hsap\MFN2^M393I.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has mitochondrion | adult stage phenotype, non-suppressible by Hsap\MFN2^R400Q.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 has cardial cell | adult stage phenotype, non-suppressible by Hsap\MFN2^R400Q.UAS, Scer\GAL4^tin.CΔ4

(Eschenbacher et al., 2012)

Suppressor of

Statement

Reference

Marf^RNAi.cUa.UAS, Scer\GAL4^VGlut-OK371 is a suppressor of mitochondrion | larval stage phenotype of Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Marf^RNAi.cUa.UAS, Scer\GAL4^VGlut-OK371 is a suppressor of embryonic/larval motor neuron | larval stage phenotype of Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Marf^RNAi.cUa.UAS, Scer\GAL4^VGlut-OK371 is a suppressor of NMJ bouton | larval stage phenotype of Drp1^{K38A.UAS.Tag:HA}, Scer\GAL4^VGlut-OK371

(El Fissi et al., 2018)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 is a suppressor of adult heart phenotype of Scer\GAL4^tin.CΔ4, park^HMS01651

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 is a suppressor of cardial cell phenotype of Scer\GAL4^tin.CΔ4, park^HMS01651

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 is a suppressor of mitochondrion phenotype of Scer\GAL4^tin.CΔ4, park^HMS01651

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 is a suppressor of adult heart phenotype of Scer\GAL4^tin.CΔ4, park^HMS01800

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 is a suppressor of cardial cell phenotype of Scer\GAL4^tin.CΔ4, park^HMS01800

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^tin.CΔ4 is a suppressor of mitochondrion phenotype of Scer\GAL4^tin.CΔ4, park^HMS01800

(Bhandari et al., 2014)

Marf^RNAi.cUa.UAS/Scer\GAL4^unspecified is a suppressor of adult thorax | dorsal phenotype of Mul1^A6, park²⁵

(Yun et al., 2014)

Marf^RNAi.cUa.UAS/Scer\GAL4^unspecified is a suppressor of mitochondrion phenotype of Mul1^A6, park²⁵

(Yun et al., 2014)

Marf^RNAi.cUa.UAS, Scer\GAL4^insc-Mz1407 is a suppressor | partially of type II neuroblast phenotype of N^GD144, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Marf^RNAi.cUa.UAS/Myc^UAS.cZa, Scer\GAL4^insc-Mz1407 is a suppressor of type II neuroblast phenotype of N^GD144, Scer\GAL4^insc-Mz1407

(Lee et al., 2013)

Marf^RNAi.cUa.UAS, Scer\GAL4^Mhc.PW is a suppressor of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2

(Wu et al., 2013)

Marf^RNAi.cUa.UAS, Scer\GAL4^Mhc.PW is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2

(Wu et al., 2013)

Marf^RNAi.cUa.UAS, Scer\GAL4^Mhc.PW is a suppressor of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A.UAS.L.Tag:FLAG}

(Wu et al., 2013)

Marf^RNAi.cUa.UAS, Scer\GAL4^Mhc.PW is a suppressor of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, trc^{K122A.UAS.L.Tag:FLAG}

(Wu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Marf^{miRNA.cUa.Scer\UAS} and park^HMS01651 under the control of Scer\GAL4^tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content usually seen in flies expressing park^HMS01651 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen in park^HMS01651, and appear fragmented as is seen with Marf^{miRNA.cUa.Scer\UAS}.

Co-expression of Marf^{miRNA.cUa.Scer\UAS} and park^HMS01800 under the control of Scer\GAL4^tin.CΔ4 suppresses the heart tube cardiomyopathy and some of the mitochondrial defects seen when either transgene is expressed alone. The proportion of reactive oxygen species (ROS)-producing and depolarized organelles is reduced, and the reduction in mitochondrial DNA content seen in flies expressing park^HMS01800 is rescued. However, the mitochondria show characteristics of both parent lines: they are still enlarged as is seen with park^HMS01800, and appear fragmented as is seen with Marf^{miRNA.cUa.Scer\UAS}.

(Bhandari et al., 2014)

The thoracic indentation phenotype of park²⁵ Mul1^A6 double mutants is almost completely suppressed by expression of Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^unspecified.

(Yun et al., 2014)

Co-expression of either Marf^{miRNA.cUa.Scer\UAS} or dm^Scer\UAS.cZa partially rescues the loss of type II neuroblasts seen in animals expressing N^GD144 under the control of Scer\GAL4^insc-Mz1407, while simultaneous co-expression of both Marf^{miRNA.cUa.Scer\UAS} and dm^Scer\UAS.cZa together fully rescues the phenotype.

(Lee et al., 2013)

The ability of rictor^Δ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is blocked by co-expression of Marf^{miRNA.cUa.Scer\UAS}.

The ability of trc^{K122A.Scer\UAS.T:Zzzz\FLAG} to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is blocked by co-expression of Marf^{miRNA.cUa.Scer\UAS}.

(Wu et al., 2013)

Co-expression of Sod^Scer\UAS.cAa rescues the heart tube defects (dilation and impaired shortening) seen in adults expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4. In addition, the morphology of the mitochondria in the cardiomyocytes is markedly improved towards wild type.

(Dorn et al., 2011)

Xenogenetic Interactions

Statement

Reference

Co-expression of Marf^{miRNA.cUa.Scer\UAS} results in a significant reduction of Pink1^{Scer\UAS.T:Ivir\HA1}-mediated neuronal rescue in Scer\GAL4^elav.PU>Hsap\HTT^{Q93.ex1p.Scer\UAS} flies.

(Khalil et al., 2015)

Co-expression of Hsap\MFN2^Scer\UAS.cDa, but not of either Hsap\MFN2^{M393I.Scer\UAS} or Hsap\MFN2^{R400Q.Scer\UAS}, rescues the reduced eye area caused by expression of Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel.

The progressive reduction in climbing ability and depressed heart tube fractional shortening of flies expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 is almost completely rescued by co-expression of Hsap\MFN2^Scer\UAS.cDa, but is not rescued by co-expression of either Hsap\MFN2^{M393I.Scer\UAS} or Hsap\MFN2^{R400Q.Scer\UAS}.

Mitochondrial fragmentation in the adult cardiomyocytes caused by expression of Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4 is almost completely suppressed by co-expression of Hsap\MFN2^Scer\UAS.cDa, but is not suppressed by co-expression of either Hsap\MFN2^{M393I.Scer\UAS} or Hsap\MFN2^{R400Q.Scer\UAS}.

(Eschenbacher et al., 2012)

Co-expression of Hsap\MFN2^Scer\UAS.cDa completely rescues the heart tube defects (dilation and impaired shortening) seen in adults expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4.

Co-expression of Hsap\MFN1^Scer\UAS.cDa can completely rescue the heart tube defects (dilation and impaired shortening) seen in adults expressing Marf^{miRNA.cUa.Scer\UAS} under the control of Scer\GAL4^tin.CΔ4, depending on the Hsap\MFN1^Scer\UAS.cDa line used.

(Dorn et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Marf^RNAi.cUa.UAS

Marf^{miRNA.cUa.Scer\UAS}

Marf^{miRNA.cUa.UAS}

Name Synonyms

Secondary FlyBase IDs

References (17)

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