FlyBase Allele Report: Dmel\Su(H)[UAS.cMa]

General Information

Symbol

Dmel\Su(H)^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Maier

FlyBase ID

FBal0270103

Feature type

allele

Associated gene

Dmel\Su(H)

Associated Insertion(s)

Carried in Construct

M{UAS-Su(H).M}

Also Known As

UAS-Su(H)

Key Links

Transgenic product class

wild_type

(Maier et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Maier et al., 2011)

Mutagen

in vitro construct

(Maier et al., 2011)

Progenitor genotype

Carried in construct

M{UAS-Su(H).M}

Cytology

Description

UAS regulatory sequences drive expression of Su(H).

(Maier et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Su(H)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size, with Scer\GAL4^bi-omb-Gal4

(Maier et al., 2013)

abnormal size | pharate adult stage, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

decreased size | adult stage, with Scer\GAL4^vg.QE

(Nagel et al., 2020)

hyperplasia | adult stage, with Scer\GAL4^GMR.PF

(Kurth et al., 2011)

hyperplasia | third instar larval stage, with Scer\GAL4^bi-omb-Gal4

(Kurth et al., 2011, Maier et al., 2011)

increased cell number, with Scer\GAL4^bi-omb-Gal4

(Maier et al., 2013)

lethal - all die during pharate adult stage, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

visible | adult stage, with Scer\GAL4^bi-omb-Gal4

(Nagel et al., 2020)

visible | adult stage, with Scer\GAL4^GMR.PF

(Kurth et al., 2011)

visible | adult stage, with Scer\GAL4^sca.PU

(Krishnan et al., 2021)

visible | adult stage, with Scer\GAL4^vg.QE

(Nagel et al., 2020)

visible | pharate adult stage, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

Phenotype Manifest In

adult external thorax, with Scer\GAL4^Bx-MS1096

(Maier et al., 2013)

chaeta | absent, with Scer\GAL4^sca.PU

(Krishnan et al., 2021)

eye, with Scer\GAL4^GMR.PF

(Kurth et al., 2011)

macrochaeta, with Scer\GAL4^Bx-MS1096

(Maier et al., 2011)

mechanosensory chaeta, with Scer\GAL4^Bx-MS1096

(Maier et al., 2013)

microchaeta, with Scer\GAL4^Bx-MS1096

(Maier et al., 2011)

tormogen cell | increased number, with Scer\GAL4^sca.PU

(Krishnan et al., 2021)

wing, with Scer\GAL4^bi-omb-Gal4

(Nagel et al., 2020)

wing | pharate adult stage, with Scer\GAL4^sd.PU

(Smylla et al., 2019)

wing disc, with Scer\GAL4^bi-omb-Gal4

(Maier et al., 2013)

wing disc | third instar larval stage, with Scer\GAL4^bi-omb-Gal4

(Kurth et al., 2011, Maier et al., 2011)

wing vein, with Scer\GAL4^vg.QE

(Nagel et al., 2020)

Detailed Description

Statement

Reference

Expressing Su(H)^UAS.cMa under the control of Scer\GAL4^sd.PU leads to enlarged wings in pharate adults raised at 18[o]C, and more severely at 25[o]C.

(Smylla et al., 2019)

Scer\GAL4^Bx-MS1096-mediated expression of Su(H)^Scer\UAS.cMa causes a transformation of shaft into socket cell fate during bristle development, affecting the majority of micro- and macrochaetae.

Scer\GAL4^bi-omb-Gal4-mediated expression of Su(H)^Scer\UAS.cMa stimulates wing discs to over-proliferate.

(Maier et al., 2013)

Expression of Su(H)^Scer\UAS.cMa in the central domain of the wing disc under the control of Scer\GAL4^bi-omb-Gal4 results in hyperproliferation.

Expression of Su(H)^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF results in overproliferation of eye tissue.

(Kurth et al., 2011)

Expression of Su(H)^Scer\UAS.cMa in the central domain of the third instar larval wing disc under the control of Scer\GAL4^bi-omb-Gal4 results in hyperproliferation.

Expression of Su(H)^Scer\UAS.cMa under the control of Scer\GAL4^Bx-MS1096 causes a mild shaft-to-socket transformation phenotype that affects most of the microchaetae and macrochaetae.

(Maier et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has lethal - all die during pharate adult stage phenotype, suppressible | partially by H^WA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has lethal - all die during pharate adult stage phenotype, suppressible | partially by H^UAS.cMb, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has lethal - all die during pharate adult stage phenotype, suppressible by H^FA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has lethal - all die during pharate adult stage phenotype, suppressible | partially by H^LLAA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has lethal - all die during pharate adult stage phenotype, non-suppressible by H^LD.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has visible | adult stage phenotype, non-suppressible by H^LD.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal size | pharate adult stage phenotype, non-suppressible by H^LD.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Scer\GAL4^GMR.PF, Su(H)^UAS.cMa has hyperplasia | adult stage phenotype, non-suppressible by H^UAS.cMb, Scer\GAL4^GMR.PF

(Kurth et al., 2011)

Scer\GAL4^GMR.PF, Su(H)^UAS.cMa has visible | adult stage phenotype, non-suppressible by H^UAS.cMb, Scer\GAL4^GMR.PF

(Kurth et al., 2011)

Scer\GAL4^bi-omb-Gal4, Su(H)^UAS.cMa has hyperplasia | third instar larval stage phenotype, non-suppressible by H^UAS.cMb, Scer\GAL4^bi-omb-Gal4

(Kurth et al., 2011)

Enhancer of

Statement

Reference

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of visible | adult stage phenotype of H^FA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of abnormal size | adult stage phenotype of H^FA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of visible | adult stage phenotype of H^LLAA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of abnormal size | adult stage phenotype of H^LLAA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of visible | adult stage phenotype of H^WA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of abnormal size | adult stage phenotype of H^WA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of visible | adult stage phenotype of H^UAS.cMb, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of abnormal size | adult stage phenotype of H^UAS.cMb, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of decreased cell number | adult stage phenotype of H^UAS.cMb, Scer\GAL4^GMR.PF

(Kurth et al., 2011)

Su(H)^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of abnormal size | adult stage phenotype of H^UAS.cMb, Scer\GAL4^GMR.PF

(Kurth et al., 2011)

Su(H)^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of H^UAS.cMb, Scer\GAL4^GMR.PF

(Kurth et al., 2011)

Other

Statement

Reference

H^LD.UAS, Scer\GAL4^bi-omb-Gal4, Su(H)^UAS.cMa has viable phenotype

(Nagel et al., 2020)

H^UAS.cMb, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has visible | adult stage phenotype

(Nagel et al., 2020)

H^UAS.cMb, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has decreased size | adult stage phenotype

(Nagel et al., 2020)

H^UAS.cMb, Scer\GAL4^bi-omb-Gal4, Su(H)^UAS.cMa has lethal - all die before end of P-stage phenotype

(Nagel et al., 2020)

H^ΔNT.UAS, Scer\GAL4^bi-omb-Gal4, Su(H)^UAS.cMa has viable phenotype

(Nagel et al., 2020)

H^ΔNT.UAS, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has visible | adult stage phenotype

(Nagel et al., 2020)

H^LD.UAS, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has visible | adult stage phenotype

(Nagel et al., 2020)

H^FA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has viable phenotype

(Smylla et al., 2019)

H^FA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has visible | adult stage phenotype

(Smylla et al., 2019)

H^FA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal size | adult stage phenotype

(Smylla et al., 2019)

H^LLAA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has visible | adult stage phenotype

(Smylla et al., 2019)

H^LLAA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal eclosion phenotype

(Smylla et al., 2019)

H^LLAA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal size | adult stage phenotype

(Smylla et al., 2019)

H^WA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has visible | adult stage phenotype

(Smylla et al., 2019)

H^WA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal eclosion phenotype

(Smylla et al., 2019)

H^WA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal size | adult stage phenotype

(Smylla et al., 2019)

H^UAS.cMb, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has visible | adult stage phenotype

(Smylla et al., 2019)

H^UAS.cMb, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal eclosion phenotype

(Smylla et al., 2019)

H^UAS.cMb, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal size | adult stage phenotype

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has visible | adult stage phenotype

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has abnormal size | adult stage phenotype

(Smylla et al., 2019)

H^UAS.cMb, Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa has lethal - all die during pharate adult stage phenotype

(Maier et al., 2013)

H^UAS.cMb, Scer\GAL4^bi-omb-Gal4, Su(H)^UAS.cMa has abnormal size phenotype

(Maier et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa has macrochaeta phenotype, non-enhanceable by H^LD.UAS, Scer\GAL4^Bx-MS1096

(Maier et al., 2011)

Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa has microchaeta phenotype, non-enhanceable by H^LD.UAS, Scer\GAL4^Bx-MS1096

(Maier et al., 2011)

Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa has macrochaeta phenotype, non-enhanceable by H^ΔNT.UAS, Scer\GAL4^Bx-MS1096

(Maier et al., 2011)

Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa has microchaeta phenotype, non-enhanceable by H^ΔNT.UAS, Scer\GAL4^Bx-MS1096

(Maier et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^sd.PU, Su(H)^UAS.cMa has wing | pharate adult stage phenotype, non-suppressible by H^LD.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Scer\GAL4^GMR.PF, Su(H)^UAS.cMa has eye phenotype, non-suppressible by H^UAS.cMb, Scer\GAL4^GMR.PF

(Kurth et al., 2011)

Enhancer of

Statement

Reference

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of wing phenotype of H^LLAA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of wing phenotype of H^WA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of wing phenotype of H^UAS.cMb, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^sd.PU is an enhancer of wing phenotype of H^FA.UAS, Scer\GAL4^sd.PU

(Smylla et al., 2019)

Su(H)^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of H^UAS.cMb, Scer\GAL4^GMR.PF

(Kurth et al., 2011)

Su(H)^UAS.cMa, Scer\GAL4^bi-omb-Gal4 is an enhancer of wing disc | third instar larval stage phenotype of H^UAS.cMb, Scer\GAL4^bi-omb-Gal4

(Kurth et al., 2011)

Other

Statement

Reference

H^ΔNT.UAS, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has wing vein phenotype

(Nagel et al., 2020)

H^UAS.cMb, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has wing phenotype

(Nagel et al., 2020)

H^UAS.cMb, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has wing vein phenotype

(Nagel et al., 2020)

H^LD.UAS, Scer\GAL4^vg.QE, Su(H)^UAS.cMa has wing vein phenotype

(Nagel et al., 2020)

H^FA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has wing phenotype

(Smylla et al., 2019)

H^FA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has scutellar bristle | ectopic phenotype

(Smylla et al., 2019)

H^LLAA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has wing phenotype

(Smylla et al., 2019)

H^LLAA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has scutellar bristle | ectopic phenotype

(Smylla et al., 2019)

H^WA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has wing phenotype

(Smylla et al., 2019)

H^WA.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has scutellar bristle | ectopic phenotype

(Smylla et al., 2019)

H^UAS.cMb, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has wing phenotype

(Smylla et al., 2019)

H^UAS.cMb, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has scutellar bristle | ectopic phenotype

(Smylla et al., 2019)

H^LD.UAS, Scer\GAL4^sd.PU, Su(H)^UAS.cMa has wing phenotype

(Smylla et al., 2019)

H^UAS.cMb, Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa has eye phenotype

(Maier et al., 2013)

H^UAS.cMb, Scer\GAL4^Bx-MS1096, Su(H)^UAS.cMa has adult head phenotype

(Maier et al., 2013)

H^UAS.cMb, Scer\GAL4^bi-omb-Gal4, Su(H)^UAS.cMa has wing disc phenotype

(Maier et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing Su(H)^UAS.cMa with one of H^FA.UAS, H^LLAA.UAS, H^WA.UAS or H^UAS.cMb results in rudimentary wings and ectopic bristle tufts mainly on the scutellum when flies are raised at 18[o]C, and complete wing loss with more scutellar bristles at 25[o]C.

Adults co-expressing Su(H)^UAS.cMa with one of H^LLAA.UAS, H^WA.UAS or H^UAS.cMb are barely viable and need assistance at eclosion from the pupal case.

(Smylla et al., 2019)

Scer\GAL4^Bx-MS1096-mediated co-expression of H^Scer\UAS.cMb and Su(H)^Scer\UAS.cMa strongly affects all N-dependent steps of bristle formation - asymmetric cell divisions as well as lateral inhibition. Bald patches, double bristles and bristle clusters are visible on the head and thorax. The eye is almost completely absent without any visible external structures. Flies die as pharate adults.

Scer\GAL4^bi-omb-Gal4-mediated co-expression of H^Scer\UAS.cMb and Su(H)^Scer\UAS.cMa leads to a very small wing disc.

(Maier et al., 2013)

Expression of Su(H)^Scer\UAS.cMa enhances the wing disc tissue loss seen when H^{Scer\UAS.attB.cMa} is expressed under the control of Scer\GAL4^bi-omb-Gal4.

Expression of H^LD.Scer\UAS does not suppress the wing disc hyperproliferation phenotype seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^bi-omb-Gal4.

Expression of Su(H)^Scer\UAS.cMa enhances the eye phenotype seen when H^{Scer\UAS.attB.cMa} is expressed under the control of Scer\GAL4^GMR.PF. Only small slits lacking ommatidial structures and eye colour remain.

Expression of H^LD.Scer\UAS does not suppress the eye hyperproliferation phenotype seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^GMR.PF.

(Kurth et al., 2011)

Co-expression of H^{Scer\UAS.attB.cMa} and Su(H)^Scer\UAS.cMa under the control of Scer\GAL4^bi-omb-Gal4 results in severely disturbed growth and development of the third instar larval wing disc.

Expression of H^LD.Scer\UAS does not enhance the wing disc proliferation defects seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^bi-omb-Gal4.

Expression of H^{ΔNT.Scer\UAS} does not enhance the wing disc proliferation defects seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^bi-omb-Gal4.

Expression of Su(H)^Scer\UAS.cMa strongly enhances the bristle loss phenotype seen when H^{Scer\UAS.attB.cMa} is expressed under the control of Scer\GAL4^Bx-MS1096. Bushes of bristles appear as a result of reduced lateral inhibition.

Expression of H^LD.Scer\UAS does not enhance the bristle phenotypes seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^Bx-MS1096.

Expression of H^{ΔNT.Scer\UAS} does not enhance the bristle phenotypes seen when Su(H)^Scer\UAS.cMa is expressed under the control of Scer\GAL4^Bx-MS1096.

(Maier et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Su(H)^Scer\UAS.cMa

Su(H)^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Maier

Secondary FlyBase IDs

References (11)

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