FlyBase Allele Report: Dmel\Neto[109]

General Information

Symbol

Dmel\Neto¹⁰⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0268741

Feature type

allele

Associated gene

Dmel\Neto

Associated Insertion(s)

Carried in Construct

Also Known As

neto^hypo

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

transposable element activity

Nature of the Allele

Allele class

Mutagen

transposable element activity

(Kim et al., 2012)

Progenitor genotype

Mi{ET1}Neto^MB05569

(Kim et al., 2012)

Cytology

Description

Imprecise excision of Mi{ET1}Neto^MB05569, resulting in removal of sequence X:13374424-13378804 , containing exon 3 (the start codon-containing exon) of Neto. Neto protein is reduced to ~40% of the wild type level in Neto¹⁰⁹ third instar larval muscle tissue. RT-PCR analyses of Neto¹⁰⁹ mRNA recovers only one truncated Neto cDNA, without the exon deleted in Neto¹⁰⁹.

(Kim et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:13,480,391..13,484,771 [+]

Comment:

4381bp deletion resulting from the imprecise excision Mi{ET1}Neto^MB05569, which removes exon 3 and the start codon.

(Kim et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

terminal bouton

(Kim et al., 2015, Sulkowski et al., 2014)

type I bouton | third instar larval stage

(Kim et al., 2012)

Detailed Description

Statement

Reference

Neto¹⁰⁹ mutants show a significant reduction in bouton number compared to controls.

(Kim et al., 2015)

Neto¹⁰⁹ mutants show a significant reduction in bouton number at muscles 6/7 and 4 NMJs compared to controls.

(Sulkowski et al., 2014)

Neto¹⁰⁹ shows 50% pupal lethality under optimal culturing conditions. Adult escapers appear morphologically normal and are fertile, but cannot fly and have severe locomotor defects. These flies cannot straighten themselves from an upside down position and show impaired walking behaviour.

The spontaneous miniature potentials from muscle 6 of third instar larvae are reduced from 6 times per second in controls to 0.3 times per second in Neto¹⁰⁹ animals. The amplitude of these mini potentials is also reduced.

The amplitude of evoked excitatory junctional potentials is reduced in Neto¹⁰⁹ third instar larvae, while the quantal content remains unaltered, compared with wild type controls.

Type I boutons at the muscle 6/7 cleft in multiple abdominal segments in Neto¹⁰⁹ third instar larvae are reduced by up to 40%. The synaptic boutons have abnormal, irregular shapes, and the post-synaptic domains exhibit gross abnormal ultrastructural profiles.

(Kim et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Neto¹⁰⁹ has partially lethal - majority live | dominant phenotype, enhanceable by mtg[+]/mtg¹

(Wang et al., 2018)

Neto¹⁰⁹ has partially lethal - majority live | dominant phenotype, enhanceable by Df(3R)BSC318/+

(Wang et al., 2018)

Neto¹⁰⁹ has partially lethal - majority live | dominant phenotype, enhanceable by Df(3R)BSC492/+

(Wang et al., 2018)

Neto¹⁰⁹ has partially lethal - majority live | dominant phenotype, enhanceable by Df(3R)BSC494/+

(Wang et al., 2018)

Neto¹⁰⁹ has partially lethal - majority die phenotype, enhanceable by gbb¹

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, enhanceable by gbb⁴

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, enhanceable by wit^unspecified

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, enhanceable by wit^B11

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, enhanceable by Mad¹

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, enhanceable by Mad^1-2

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, enhanceable by Df(2L)C28

(Sulkowski et al., 2014)

NOT Enhanced by

Statement

Reference

Neto¹⁰⁹ has partially lethal - majority die phenotype, non-enhanceable by put⁶²

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, non-enhanceable by put¹³⁵

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, non-enhanceable by put⁶²/wit^G15

(Sulkowski et al., 2014)

Neto¹⁰⁹ has partially lethal - majority die phenotype, non-enhanceable by wit^G4/put¹³⁵

(Sulkowski et al., 2014)

NOT suppressed by

Statement

Reference

Neto¹⁰⁹ has abnormal neuroanatomy phenotype, non-suppressible by gbb^UAS.cKa/Scer\GAL4^elav.PLu

(Sulkowski et al., 2014)

Neto¹⁰⁹ has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^G14/gbb^UAS.cKa

(Sulkowski et al., 2014)

Other

Statement

Reference

Neto¹⁰⁹, mtg[+]/mtg¹ has lethal | dominant phenotype

(Wang et al., 2018)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Neto¹⁰⁹ has terminal bouton phenotype, non-suppressible by gbb^UAS.cKa/Scer\GAL4^elav.PLu

(Sulkowski et al., 2014)

Neto¹⁰⁹ has terminal bouton phenotype, non-suppressible by Scer\GAL4^G14/gbb^UAS.cKa

(Sulkowski et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of gbb^Scer\UAS.cKa under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^G14 does not suppress the reduction in NMJ bouton number seen in Neto¹⁰⁹ mutant larvae.

(Sulkowski et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

The defects in flight and climbing abilities of Neto¹⁰⁹ flies are completely rescued by Dp(1;3)DC270.

Defects in spontaneous miniature potential frequency and amplitude in Neto¹⁰⁹/Neto³⁶ animals are rescued by Dp(1;3)DC270.

The reduced amplitude of evoked excitatory junctional potentials in Neto¹⁰⁹ third instar larvae is rescued by Dp(1;3)DC270.

The reduced number of synaptic boutons seen in Neto¹⁰⁹ third instar larvae is rescued by Dp(1;3)DC270.

(Kim et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Neto¹⁰⁹

neto¹⁰⁹

(Muttathukunnel et al., 2022, Wang et al., 2018, Kim et al., 2015, Ramos et al., 2015, Sulkowski et al., 2014, Kim et al., 2012)

neto^hypo

(Han et al., 2020, Ramos et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (7)

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