FlyBase Allele Report: Dmel\Hcf[HR1]

General Information

Symbol

Dmel\Hcf^HR1

Species

D. melanogaster

Name

FlyBase ID

FBal0267506

Feature type

allele

Associated gene

Dmel\Hcf

Associated Insertion(s)

TI{TI}Hcf^HR1

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Rodriguez-Jato et al., 2011)

Mutagen

ends-out gene targeting

(Rodriguez-Jato et al., 2011, Rodriguez-Jato et al., 2011)

Progenitor genotype

Associated Insertion(s)

TI{TI}Hcf^HR1

Cytology

Description

Hcf sequence extending from -344 to +4004 relative to the transcription start site has been replaced by a w^+mW.hs marker flanked by loxP sites.

(Rodriguez-Jato et al., 2011)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage

(Rodriguez-Jato et al., 2011)

decreased body size | pupal stage

(Rodriguez-Jato et al., 2011)

decreased cell size

(Rodriguez-Jato et al., 2011)

female sterile

(Rodriguez-Jato et al., 2011)

increased cell death | third instar larval stage

(Rodriguez-Jato et al., 2011)

male semi-sterile

(Rodriguez-Jato et al., 2011)

partially lethal - majority die

(Rodriguez-Jato et al., 2011)

some die during pupal stage

(Rodriguez-Jato et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

A significant proportion of Hcf^HR1 homozygous adults show ectopic wing veins and ectopic scutellar bristles, as compared to controls.

(Mariappa et al., 2018)

Homozygotes survive until the pupal stage, where they show approximately 50% lethality. Of those animals surviving to adulthood, 30% of males and 100% of females are sterile. Homozygous pupae and adults are smaller than normal. The wings are reduced in size, at least partly because of a reduction in cell size.

Homozygous wing discs show normal cell proliferation, but have increased levels of apoptosis compared to wild type.

The ovaries of homozygous females are usually underdeveloped. Egg chamber degeneration at around stage 8 of oogenesis is seen. The females lay some eggs, which are often fragile and smaller than normal, and have abnormally shaped anterior termini and dorsal appendages.

Homozygous adults show loss of humeral bristles (60% penetrance), held out wings (30% penetrance) and an ectopic vein initiating from the posterior crossvein in the wing (60% penetrance). Homozygous males have a reduced number of sex comb teeth (80% penetrance for 9 or fewer teeth/sex comb).

(Rodriguez-Jato et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Hcf^HR1 has decreased body size | adult stage phenotype, non-enhanceable by E(z)[+]/E(z)^su301

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has decreased body size | adult stage phenotype, non-enhanceable by Pc¹/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has decreased body size | adult stage phenotype, non-enhanceable by Pc³/Pc[+]

(Rodriguez-Jato et al., 2011)

NOT suppressed by

Statement

Reference

Hcf^HR1 has decreased body size | adult stage phenotype, non-suppressible by Pc¹/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has decreased body size | adult stage phenotype, non-suppressible by Pc³/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has decreased body size | adult stage phenotype, non-suppressible by E(z)[+]/E(z)^su301

(Rodriguez-Jato et al., 2011)

Enhancer of

Statement

Reference

Hcf[+]/Hcf^HR1 is an enhancer of visible phenotype of Pc¹

(Rodriguez-Jato et al., 2011)

Hcf[+]/Hcf^HR1 is an enhancer of visible phenotype of Pc³

(Rodriguez-Jato et al., 2011)

Other

Statement

Reference

Hcf^HR1, brm¹/brm[+] has visible phenotype

(Rodriguez-Jato et al., 2011)

Hcf^HR1, brm²⁰/brm[+] has visible phenotype

(Rodriguez-Jato et al., 2011)

Hcf^HR1, mor[+]/mor¹ has visible phenotype

(Rodriguez-Jato et al., 2011)

Asx^XF23/Asx[+], Hcf^HR1 has visible phenotype

(Rodriguez-Jato et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hcf^HR1 has wing vein | ectopic phenotype, enhanceable by sxc^H537A/sxc^H537A

(Mariappa et al., 2018)

Hcf^HR1 has scutellar bristle | ectopic phenotype, enhanceable by sxc^H537A/sxc^H537A

(Mariappa et al., 2018)

Hcf^HR1 has wing vein | ectopic phenotype, enhanceable by skd¹/skd[+]

(Rodriguez-Jato et al., 2011)

NOT Enhanced by

Statement

Reference

Hcf^HR1 has humeral bristle phenotype, non-enhanceable by E(z)[+]/E(z)^su301

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has wing vein | ectopic phenotype, non-enhanceable by E(z)[+]/E(z)^su301

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has humeral bristle phenotype, non-enhanceable by Pc¹/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has sex comb phenotype, non-enhanceable by Pc¹/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has humeral bristle phenotype, non-enhanceable by Pc³/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has sex comb phenotype, non-enhanceable by Pc³/Pc[+]

(Rodriguez-Jato et al., 2011)

Suppressed by

Statement

Reference

Hcf^HR1 has wing vein | ectopic phenotype, suppressible by Pc³/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has wing vein | ectopic phenotype, suppressible by Pc¹/Pc[+]

(Rodriguez-Jato et al., 2011)

NOT suppressed by

Statement

Reference

Hcf^HR1 has humeral bristle phenotype, non-suppressible by Pc¹/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has humeral bristle phenotype, non-suppressible by Pc³/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has humeral bristle phenotype, non-suppressible by E(z)[+]/E(z)^su301

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has sex comb phenotype, non-suppressible by Pc¹/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has sex comb phenotype, non-suppressible by Pc³/Pc[+]

(Rodriguez-Jato et al., 2011)

Hcf^HR1 has wing vein | ectopic phenotype, non-suppressible by E(z)[+]/E(z)^su301

(Rodriguez-Jato et al., 2011)

Enhancer of

Statement

Reference

Hcf^HR1/Hcf^HR1 is an enhancer of wing vein | ectopic phenotype of sxc^H537A

(Mariappa et al., 2018)

Hcf[+]/Hcf^HR1 is an enhancer of mesothoracic preepisternum phenotype of Pc¹

(Rodriguez-Jato et al., 2011)

Hcf[+]/Hcf^HR1 is an enhancer of mesothoracic preepisternum phenotype of Pc³

(Rodriguez-Jato et al., 2011)

Other

Statement

Reference

Hcf^HR1, brm¹/brm[+] has leg phenotype

(Rodriguez-Jato et al., 2011)

Hcf^HR1, skd¹/skd[+] has scutellar bristle | ectopic phenotype

(Rodriguez-Jato et al., 2011)

Hcf^HR1, brm²⁰/brm[+] has leg phenotype

(Rodriguez-Jato et al., 2011)

Hcf^HR1, mor[+]/mor¹ has leg phenotype

(Rodriguez-Jato et al., 2011)

Hcf^HR1, Pc¹/Pc[+] has male genitalia phenotype

(Rodriguez-Jato et al., 2011)

Hcf^HR1, Pc³/Pc[+] has male genitalia phenotype

(Rodriguez-Jato et al., 2011)

Asx^XF23/Asx[+], Hcf^HR1 has male genitalia phenotype

(Rodriguez-Jato et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

sxc^H537A, Hcf^HR1 double homozygous adults show a more prevalent ectopic wing vein phenotype than either single homozygotes and a more prevalent ectopic scutellar bristle phenotype than the Hcf^HR1 single homozygotes.

(Mariappa et al., 2018)

Hcf^HR1 shows leg defects when in double heterozygous combination with brm¹ (20% penetrance), brm²⁰ (10% penetrance) or mor¹ (10% penetrance).

Hcf^HR1/+ enhances the penetrance of the T2 to T1 transformation (reduction of the sternopleural region) seen in Pc¹/+ and Pc³/+ flies, from 3% to 60% and from 3% to 40% respectively.

The adult size and humeral bristle phenotypes of Hcf^HR1 mutants are not affected by Pc¹/+, Pc³/+ or E(z)^su301/+. The reduced sex comb phenotype of Hcf^HR1 is not modified by Pc¹/+ or Pc³/+. The ectopic vein phenotype of Hcf^HR1 mutants is suppressed by Pc¹/+ and by Pc³/+, whereas it is unaffected by E(z)^su301/+.

3% of Pc¹/+ ; Hcf^HR1 males, 4% of Pc³/+ ; Hcf^HR1 males and 2% of Asx^XF23/+ ; Hcf^HR1 males have defects in rotation of the genitalia.

30% of skd¹/+ ; Hcf^HR1 males have defects in rotation of the genitalia. 30% of skd¹/+ ; Hcf^HR1 flies have extra scutellar bristles. The expressivity of the ectopic wing vein phenotype seen in Hcf^HR1 mutants is increased by skd¹/+.

(Rodriguez-Jato et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Hcf^HR1 is rescued by Scer\GAL4^Act.PU/Hcf^{UAS.Tag:T7,Tag:FLAG}

(Rodriguez-Jato et al., 2011)

Comments

Expression of Hcf^{Scer\UAS.T:Uuuu\T7,T:Zzzz\FLAG} under the control of Scer\GAL4^Act.PU at 18[o]C rescues the reduced body size, loss of humeral bristles, held-out wings and ectopic veins seen in Hcf^HR1 animals.

(Rodriguez-Jato et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

39627

w^67c23; TI{TI}Hcf^HR1/In(4)ci^D, ci^D pan^ciD

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hcf^HR1

(Mariappa et al., 2018)

dHCF^HR1

(Rodriguez-Jato et al., 2011, Rodriguez-Jato et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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