FlyBase Allele Report: Dmel\ck[KT9]

General Information

Symbol

Dmel\ck^KT9

Species

D. melanogaster

Name

FlyBase ID

FBal0267128

Feature type

allele

Associated gene

Dmel\ck

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Bejsovec and Chao, 2012)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Bejsovec and Chao, 2012)

Amino acid replacement: Q445term.

(Bejsovec and Chao, 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:15,050,960..15,050,960 [-]

Nucleotide change:

C15050960T

Reported nucleotide change:

C?T

Amino acid change:

Q445term | ck-PA; Q445term | ck-PB; Q445term | ck-PC; Q445term | ck-PD

Reported amino acid change:

Q445term

(Bejsovec and Chao, 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage

(Bejsovec and Chao, 2012)

some die during larval stage

(Bejsovec and Chao, 2012)

Phenotype Manifest In

dorsal fine hair

(Bejsovec and Chao, 2012)

Detailed Description

Statement

Reference

Hemizygous embryos do not have any obvious denticle morphology defects in the denticle belts. The fine hairs on the dorsal surface of the cuticle are dramatically truncated and frequently misorientated.

Apical actin aggregations in the ventral epidermal cells do not mature into the normal final denticle shape in homozygous embryos, remaining rounded even at and after 14 hours after egg laying.

(Bejsovec and Chao, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

ck^KT9, f¹ has denticle phenotype

(Bejsovec and Chao, 2012)

ck^KT9, f¹, m¹ has denticle phenotype

(Bejsovec and Chao, 2012)

ck^KT9, m¹ has denticle phenotype

(Bejsovec and Chao, 2012)

Scer\GAL4^e22c, ck^KT9, ovo^svb.UAS has denticle phenotype

(Bejsovec and Chao, 2012)

ck^KT9, wg^PE2 has denticle phenotype

(Bejsovec and Chao, 2012)

ck^KT9, wg^l-12 has denticle | heat sensitive phenotype

(Bejsovec and Chao, 2012)

ck^KT9, wg^l-17 has denticle phenotype

(Bejsovec and Chao, 2012)

ck^KT9, pan², wg^l-17 has denticle phenotype

(Bejsovec and Chao, 2012)

SoxN^NC14, ck^KT9, wg^l-17 has denticle phenotype

(Bejsovec and Chao, 2012)

Scer\GAL4^e22c, ck^KT9, m^EP406 has denticle phenotype

(Bejsovec and Chao, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

ck^KT9 modifies the wg^l-17 denticle phenotype, such that the double mutants have denticles with a rounded appearance and no distinctive hook.

In wg^l-17 ck^KT9 ; pan² triple mutants, smaller anterior denticles can have sharp points, while larger posterior denticles remain rounded and some appear fragmented.

wg^l-17 ck^KT9 SoxN^NC14 triple mutant embryos show a dramatic reduction in denticle size and many of the denticles are fragmented into one or two short projections. Actin bundles in the epidermal cells are split into two or sometimes three separate aggregates.

wg^l-12 ck^KT9 double mutant embryos shifted to the restrictive temperature at 9.5 hours after egg laying (AEL) have similar cuticle patterning defects as wg^l-12 single mutant embryos shifted at the same time, but in addition, many denticles remain rounded in appearance in the double mutants, particularly the large posterior denticles. The row 5 denticles are not properly elongated or hooked in double mutant embryos shifted to the restrictive temperature at 10 hours AEL.

m¹ ; ck^KT9 double mutants show a reduction in both size and sharpness of the denticles compared to either single mutant.

f¹ ; ck^KT9 double mutants show a slight reduction in both size and sharpness of the denticles compared to either single mutant.

m¹ f¹ ck^KT9 triple mutants show almost no elongation of the denticles.

Expression of m^EP406 under the control of Scer\GAL4^e22c in a ck^KT9 background results in a strong reduction in denticle size.

Expression of ovo^svb.Scer\UAS under the control of Scer\GAL4^e22c in a ck^KT9 background causes a strong reduction in denticle size and results in a change in denticle morphology, while the ectopic denticles found between the denticle belts in animals expressing ovo^svb.Scer\UAS under the control of Scer\GAL4^e22c in a wild-type background are virtually eliminated.

(Bejsovec and Chao, 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ck^KT9

(Sallee et al., 2021, Bejsovec and Chao, 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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