FlyBase Allele Report: Dmel\His3[HisGU.wt]

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Allele: Dmel\His3^HisGU.wt

General Information

Symbol

Dmel\His3^HisGU.wt

Species

D. melanogaster

Name

FlyBase ID

FBal0266780

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

PBac{3xHisGU.wt}

Also Known As

6xHisGU

Key Links

Transgenic product class

(Günesdogan et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Günesdogan et al., 2010)

Mutagen

in vitro construct

(Günesdogan et al., 2010)

Progenitor genotype

Carried in construct

M{3xHisGU.H2A^4K-4R}

M{3xHisGU.H4K16A}

M{3xHisGU.H4K16Q}

M{3xHisGU.H4K16R}

PBac{3xHisGU.H2A^4K-4R}

PBac{3xHisGU.H4K16Q}

PBac{3xHisGU.H4K16R}

PBac{3xHisGU.wt}

PBac{6xHisGU.wt}

Cytology

Description

Wild-type His3 gene in the context of a histone gene repeat unit.

(Günesdogan et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The sensory bristles in Df(2L)His^C 4xM{3xHisGU.wt} mutant flies appear indistinguishable from controls.

(Pengelly et al., 2013)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16R, His4r^Δ has partially lethal - majority die | female limited phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16R, His4r^Δ has short lived | female limited phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16R, His4r^Δ has lethal - all die before end of larval stage | male limited phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.wt, His4r^Δ has fertile phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.wt, His4r^Δ has partially lethal - majority die phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16Q, His4r^Δ has partially lethal - majority die phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16Q, His4r^Δ has short lived phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16A, His4r^Δ has lethal - all die during embryonic stage phenotype

(Copur et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Df(2L)His^C, His4r^Δ double homozygotes rescued by 12x copies of the HisGU gene unit (bearing His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt and His4^HisGU.wt) are semi-lethal and adults are fertile and show normal lifespan.

Epidermal Df(2L)His^C clones in His4r^Δ homozygotes that also express variants of the HisGU gene unit (12 copies; bearing His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt and His3^HisGU.wt with either His4^HisGU.wt, His4^HisGU.H4K16R, His4^HisGU.H4K16Q or His4^HisGU.H4K16A) are able to form normal-looking adult structures with sensory bristles.

(Copur et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

His3^HisGU.wt rescues Df(2L)His^C

(Graves et al., 2016)

His3^HisGU.wt rescues Df(2L)His^C

(Pengelly et al., 2013)

Comments

12 copies of a histone repeat unit (flies contain 4 copies of PBac{3xHisGU.wt}) largely rescues the embryonic lethality of Df(2L)His^C homozygotes to adulthood (some pupal lethality is seen).

12 copies of a histone repeat unit (flies contain 4 copies of PBac{3xHisGU.wt}) rescue the severe growth defect seen in homozygous Df(2L)His^C clones in the wing disc.

(Graves et al., 2016)

Clones containing Df(2L)His^C mutant cells stop proliferating, but this phenotype is rescued in animals that also carry four copies of M{3xHisGU.wt}.

(Pengelly et al., 2013)

Increasing copy numbers of the histone gene repeat unit provided by transgenic constructs can rescue the Df(2L)His^C phenotype: Df(2L)His^C homozygotes that are also homozygous for M{1xHisGU.wt} develop a defective cuticle. Df(2L)His^C homozygotes that are also homozygous for M{3xHisGU.wt} develop a wild-type cuticle pattern but fail to hatch. Df(2L)His^C homozygotes that carry four copies of M{3xHisGU.wt} (homozygous for two different insertions of M{3xHisGU.wt}) are rescued to become fertile adults with no visible morphological defects.

(Günesdogan et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

65301

w^*; PBac{3xHisGU.wt}VK00027 PBac{3xHisGU.wt}VK00033/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

His3^HisGU.wt

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections