FlyBase Allele Report: Dmel\His2B[HisGU.wt]

General Information

Symbol

Dmel\His2B^HisGU.wt

Species

D. melanogaster

Name

FlyBase ID

FBal0266779

Feature type

allele

Associated gene

His2B

Associated Insertion(s)

Carried in Construct

M{1xHisGU.wt}

M{3xHisGU.H3K27R}

M{3xHisGU.wt}

Also Known As

6xHisGU

Key Links

Transgenic product class

wild_type

(Günesdogan et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Günesdogan et al., 2010)

Mutagen

in vitro construct

(Günesdogan et al., 2010)

Progenitor genotype

Cytology

Description

Wild-type His2B gene in the context of a histone gene repeat unit.

(Günesdogan et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

His2B

Encoded product / tool

His2B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The sensory bristles in Df(2L)His^C 4xM{3xHisGU.wt} mutant flies appear indistinguishable from controls.

(Pengelly et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16R, His4r^Δ has partially lethal - majority die | female limited phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16R, His4r^Δ has short lived | female limited phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16R, His4r^Δ has lethal - all die before end of larval stage | male limited phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.wt, His4r^Δ has fertile phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.wt, His4r^Δ has partially lethal - majority die phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16Q, His4r^Δ has partially lethal - majority die phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16Q, His4r^Δ has short lived phenotype

(Copur et al., 2018)

Df(2L)His^C, His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt, His4^HisGU.H4K16A, His4r^Δ has lethal - all die during embryonic stage phenotype

(Copur et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Df(2L)His^C, His4r^Δ double homozygotes rescued by 12x copies of the HisGU gene unit (bearing His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt, His3^HisGU.wt and His4^HisGU.wt) are semi-lethal and adults are fertile and show normal lifespan.

Epidermal Df(2L)His^C clones in His4r^Δ homozygotes that also express variants of the HisGU gene unit (12 copies; bearing His1^HisGU.wt, His2A^HisGU.wt, His2B^HisGU.wt and His3^HisGU.wt with either His4^HisGU.wt, His4^HisGU.H4K16R, His4^HisGU.H4K16Q or His4^HisGU.H4K16A) are able to form normal-looking adult structures with sensory bristles.

(Copur et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

His2B^HisGU.wt rescues Df(2L)His^C

(Pengelly et al., 2013)

Comments

Clones containing Df(2L)His^C mutant cells stop proliferating, but are rescued in animals that also carry four copies of M{3xHisGU.wt}.

(Pengelly et al., 2013)

Increasing copy numbers of the histone gene repeat unit provided by transgenic constructs can rescue the Df(2L)His^C phenotype: Df(2L)His^C homozygotes that are also homozygous for M{1xHisGU.wt} develop a defective cuticle. Df(2L)His^C homozygotes that are also homozygous for M{3xHisGU.wt} develop a wild-type cuticle pattern but fail to hatch. Df(2L)His^C homozygotes that carry four copies of M{3xHisGU.wt} (homozygous for two different insertions of M{3xHisGU.wt}) are rescued to become fertile adults with no visible morphological defects.