FB2024_03 , released June 25, 2024
Recombinant Construct: M{3xHisGU.H3K27R}
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General Information
Symbol
M{3xHisGU.H3K27R}
FlyBase ID
FBtp0070985
Feature type
transgenic_transposable_element
Component Allele(s)
His1HisGU.wt
His2AHisGU.wt
His2BHisGU.wt
His3HisGU.H3K27R
His4HisGU.wt
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele His1HisGU.wt
Transgenic product class
wild_type
(Günesdogan et al., 2010)
Component
Use(s)
Regulatory region(s)
His1
Encoded product / tool
His1
Description

Wild-type His1 gene in the context of a histone gene repeat unit.

(Günesdogan et al., 2010)
Component allele His2AHisGU.wt
Transgenic product class
wild_type
(Günesdogan et al., 2010)
Component
Use(s)
Regulatory region(s)
His2A
Encoded product / tool
His2A
Description

Wild-type His2A gene in the context of a histone gene repeat unit.

(Günesdogan et al., 2010)
Component allele His2BHisGU.wt
Transgenic product class
wild_type
(Günesdogan et al., 2010)
Component
Use(s)
Regulatory region(s)
His2B
Encoded product / tool
His2B
Description

Wild-type His2B gene in the context of a histone gene repeat unit.

(Günesdogan et al., 2010)
Component allele His3HisGU.H3K27R
Transgenic product class
missense_variant
(Günesdogan et al., 2010)
Component
Use(s)
Regulatory region(s)
His3
Encoded product / tool
His3
Description

Mutated His3 gene (amino acid replacement K27R, to prevent methylation) in the context of a histone gene repeat unit.

(Günesdogan et al., 2010)
Component allele His4HisGU.wt
Transgenic product class
wild_type
(Günesdogan et al., 2010)
Component
Use(s)
Regulatory region(s)
His4
Encoded product / tool
His4
Description

Wild-type His4 gene in the context of a histone gene repeat unit.

(Günesdogan et al., 2010)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments

Contains three copies of the histone gene repeat unit (each repeat contains one copy of each of His1, His2A, His2B, His3 and His4). Each copy of the His3 gene has been mutated to contain the amino acid replacement K27R (to prevent methylation).

(Günesdogan et al., 2010)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
Reported As
Symbol Synonym
12xH4K16R
(Samata et al., 2020)
12xH4wt
(Samata et al., 2020)
12xHisGu-H4K16R
(Samata et al., 2020)
12xHisGu-H4wt
(Samata et al., 2020)
3xHisGUH3K27R
(Finogenova et al., 2020)
H4K16R
(Samata et al., 2020)
H4wt
(Samata et al., 2020)
M{3xHisGU.H3K27R}
(Günesdogan et al., 2010, FlyBase, 1996-)
Secondary FlyBase IDs
    References (5)