FB2024_03 , released June 25, 2024
Allele: Dmel\shot2A2-4
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General Information
Symbol
Dmel\shot2A2-4
Species
D. melanogaster
Name
FlyBase ID
FBal0266751
Feature type
allele
Associated gene
Dmel\shot
Associated Insertion(s)
Carried in Construct
Also Known As
shot2A2
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Chang et al., 2011)
Progenitor genotype
Cytology
Description

Amino acid replacement: V224D.

(Nashchekin et al., 2016)

Coordinates of amino acid replacement are relative to isoform PE.

(Nashchekin et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2R:13,916,936..13,916,936 [-]
Nucleotide change:

T13916936A

Reported nucleotide change:

T?A

Amino acid change:

V113D | shot-PK; V224D | shot-PO; V224D | shot-PE; V224D | shot-PA; V133D | shot-PAA; V113D | shot-PJ; V113D | shot-PI; V224D | shot-PL; V224D | shot-PM; V113D | shot-PB; V119D | shot-PZ; V113D | shot-PG; V113D | shot-PN

Reported amino acid change:

V224D

Comment:

The shot2A2-4 amino acid change was reported relative to the shot-PE isoform.

(Nashchekin et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The polarized (posterior) localization of fate determinants (osk mRNA) and markers of kinesin-dependent transport activity (Dynein and Glued) is abolished in oocytes from shot2A2-4 mutant germline clones and the microtubule organization (their anterior enrichment) is perturbed compared to wild-type.

      (Nashchekin et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      shot2A2-4/shot[+] is a suppressor of embryonic head phenotype of miraαTub67C.mGFP6

      (Chang et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The bicaudal phenotype of embryos derived from miraαTub67C.T:Avic\GFP-m6/+ females is suppressed if the females are also heterozygous for shot2A2-4.

      (Chang et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      2A2-4
      (Chang et al., 2011)
      shot2A2-4
      shot2A2
      (Nashchekin et al., 2024, Nashchekin et al., 2016, Trovisco et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)