FlyBase Allele Report: Dmel\Nak[RNAi.UAS]

General Information

Symbol

Dmel\Nak^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0264517

Feature type

allele

Associated gene

Dmel\Nak

Associated Insertion(s)

Carried in Construct

P{UAS-Nak.RNAi}

Also Known As

UAS-nak-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Peng et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Peng et al., 2009)

Mutagen

in vitro construct

(Peng et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-Nak.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 434bp Nak cDNA sequence (spanning 198bp preceding the stop codon and 237bp of the 3' UTR).

(Peng et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Nak

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendrite | larval stage, with Df(2L)TW3, Scer\GAL4^elav-C155

(Yang et al., 2011)

dendrite | larval stage, with Scer\GAL4^109(2)80

(Yang et al., 2011)

dendrite | larval stage, with Scer\GAL4^elav-C155

(Yang et al., 2011)

dendrite | larval stage, with Scer\GAL4^pk-IG1-1

(Yang et al., 2011)

dendrite | larval stage | somatic clone, with Scer\GAL4⁴⁷⁷

(Yang et al., 2011)

dendritic arborizing neuron | larval stage, with Df(2L)TW3, Scer\GAL4^elav-C155

(Yang et al., 2011)

dendritic arborizing neuron | larval stage, with Scer\GAL4^109(2)80

(Yang et al., 2011)

dendritic arborizing neuron | larval stage, with Scer\GAL4^elav-C155

(Yang et al., 2011)

dendritic spine | larval stage, with Df(2L)TW3, Scer\GAL4^elav-C155

(Yang et al., 2011)

larval dorsal multidendritic neuron ddaA | larval stage, with Df(2L)TW3, Scer\GAL4^elav-C155

(Yang et al., 2011)

larval dorsal multidendritic neuron ddaC | larval stage | somatic clone, with Scer\GAL4⁴⁷⁷

(Yang et al., 2011)

larval dorsal multidendritic neuron ddaE | larval stage, with Scer\GAL4^pk-IG1-1

(Yang et al., 2011)

Detailed Description

Statement

Reference

The number of dendritic endpoints of dorsal dendritic arborisation neurons is reduced compared to controls in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^109(2)80. Analysis of branching in second instar larvae (when dendrites are undergoing arborisation) suggests that the defects are due to disruption of the formation of new dendritic branches and disruption of the extension of existing branches, while dendrite retraction appears to be unaffected.

Single cell ddaC neuron clones expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4⁴⁷⁷ show shortening and reduction of dendritic branches, with higher order dendrites being preferentially affected.

The number and the length of dendritic spikes in class III ddaA neurons is reduced in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155 and carrying Df(2L)TW3 compared to controls. In addition, the number of dendritic endpoints of dorsal dendritic arborisation neurons is reduced compared to controls in these larvae.

Expression of Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^IG1-1 results in a reduction in the number and length of higher order (tertiary or higher branch) dendrites of class I ddaE neurons.

The number of dendritic endpoints of dorsal dendritic arborisation neurons is reduced compared to controls in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155.

(Yang et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Nrg^l7/Nrg[+]

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has abnormal neuroanatomy | larval stage phenotype, enhanceable by AP-2α³/alpha-Adaptin[+]

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Chc[+]/Chc¹

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has abnormal neuroanatomy | larval stage phenotype, enhanceable by Nrg^l4/Nrg[+]

(Yang et al., 2011)

NOT Enhanced by

Statement

Reference

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by AP-47[+]/AP-1μ^SAE-10

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has abnormal neuroanatomy | larval stage phenotype, non-enhanceable by g[+]/g¹

(Yang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has dendritic arborizing neuron | larval stage phenotype, enhanceable by AP-2α³/alpha-Adaptin[+]

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has dendrite | larval stage phenotype, enhanceable by AP-2α³/alpha-Adaptin[+]

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has dendrite | larval stage phenotype, enhanceable by Nrg^l7/Nrg[+]

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has dendritic arborizing neuron | larval stage phenotype, enhanceable by Chc[+]/Chc¹

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has dendrite | larval stage phenotype, enhanceable by Chc[+]/Chc¹

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has dendritic arborizing neuron | larval stage phenotype, enhanceable by Nrg^l4/Nrg[+]

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has dendrite | larval stage phenotype, enhanceable by Nrg^l4/Nrg[+]

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^elav-C155 has dendritic arborizing neuron | larval stage phenotype, enhanceable by Nrg^l7/Nrg[+]

(Yang et al., 2011)

NOT Enhanced by

Statement

Reference

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has dendritic arborizing neuron | larval stage phenotype, non-enhanceable by AP-47[+]/AP-1μ^SAE-10

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has dendrite | larval stage phenotype, non-enhanceable by AP-47[+]/AP-1μ^SAE-10

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has dendritic arborizing neuron | larval stage phenotype, non-enhanceable by g[+]/g¹

(Yang et al., 2011)

Nak^RNAi.UAS, Scer\GAL4^109(2)80 has dendrite | larval stage phenotype, non-enhanceable by g[+]/g¹

(Yang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

α-Adaptin³/+ enhances the reduction in the number of dendritic endpoints of dorsal dendritic arborisation neurons which is seen in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155. In addition, clusters of shortened terminals are more often seen.

Chc¹/+ enhances the reduction in the number of dendritic endpoints of dorsal dendritic arborisation neurons which is seen in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^109(2)80. In addition, clusters of shortened terminals are more often seen.

Nrg^l4 and Nrg^l7 each dominantly enhance the shortening of dendritic length and the reduction in the number of dendritic endpoints of dorsal dendritic arborisation neurons which is seen in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155.

The reduction in the number of dendritic endpoints of dorsal dendritic arborisation neurons which is seen in larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^109(2)80 is not enhanced by either AP-47^SAE-10/+ or g¹/+.

(Yang et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nak^RNAi.UAS is rescued by Scer\GAL4^109(2)80/Nak^UAS.cCa

(Yang et al., 2011)

Nak^RNAi.UAS is rescued by Scer\GAL4^elav-C155/Nak^UAS.cCa

(Yang et al., 2011)

Not rescued by

Nak^RNAi.UAS is not rescued by Scer\GAL4^109(2)80/Nak^{KD.UAS.Tag:MYC}

(Yang et al., 2011)

Nak^RNAi.UAS is not rescued by Scer\GAL4^elav-C155/Nak^{KD.UAS.Tag:MYC}

(Yang et al., 2011)

Nak^RNAi.UAS is not rescued by Scer\GAL4^109(2)80/Nak^{DPF-AAA.UAS.Tag:MYC}

(Yang et al., 2011)

Nak^RNAi.UAS is not rescued by Scer\GAL4^elav-C155/Nak^{DPF-AAA.UAS.Tag:MYC}

(Yang et al., 2011)

Comments

Co-expression of Nak^Scer\UAS.cCa rescues the dendritic defects seen in the dorsal dendritic arborisation neurons of larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^109(2)80.

Co-expression of Nak^Scer\UAS.cCa rescues the dendritic defects seen in the dorsal dendritic arborisation neurons of larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav-C155.

Co-expression of either Nak^{KD.Scer\UAS.T:Hsap\MYC} or Nak^{DPF-AAA.Scer\UAS.T:Hsap\MYC} fails to rescue the dendritic defects seen in the dorsal dendritic arborisation neurons of larvae expressing Nak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^109(2)80.

(Yang et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nak^RNAi.UAS

Nak^{dsRNA.Scer\UAS}

Nak^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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