FB2024_03 , released June 25, 2024
Allele: Dmel\pblΔN-term.UAS.Tag:HA
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General Information
Symbol
Dmel\pblΔN-term.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0263872
Feature type
allele
Associated gene
Dmel\pbl
Associated Insertion(s)
Carried in Construct
P{UAS-pbl.ΔN-term.HA}
Key Links
Transgenic product class
inframe_deletion
(van Impel et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(van Impel et al., 2009)
Mutagen
in vitro construct
(van Impel et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-pbl.ΔN-term.HA}
Cytology
Description

UASt regulatory sequences drive expression of a mutated form of pbl-RA cDNA (amino acids 386-853) that lacks the N-terminal regulatory domains and is tagged at the C-terminal end with Tag:HA.

(van Impel et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
pbl
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4e22c-mediated expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 results in 100% embryonic lethality and epidermal fragmentation.

      (Greer et al., 2013)

      Expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 in the eye under the control of the Scer\GAL4GMR.PU driver results in pupal lethality. At 18[o]C the lethality occurs at the pharate adult stage. Pupal flies exhibit a strong eye phenotype.

      Expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 in the mesoderm of stage 8 embryos under the control of Scer\GAL4twi.2PE and Scer\GAL4Mef2.PR drivers blocks mesoderm invagination and the cells fail to undergo cytokinesis.

      (van Impel et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has lethal | pharate adult stage | temperature conditional phenotype, suppressible by Rho1k07236/Rho1[+]

      (van Impel et al., 2009)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has lethal | pharate adult stage | temperature conditional phenotype, non-suppressible by Rac2Δ/Mtl[+]/Rac1J10/MtlΔ/Rac2[+]/Rac1[+]

      (van Impel et al., 2009)
      Other
      Statement
      Reference

      Rho1k07236/Rho1[+], Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has visible | adult stage | temperature conditional phenotype

      (van Impel et al., 2009)
      Phenotype Manifest In
      Other
      Statement
      Reference

      Rho1k07236/Rho1[+], Scer\GAL4GMR.PU, pblΔN-term.UAS.Tag:HA has eye | adult stage | temperature conditional phenotype

      (van Impel et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of Rho1k07236 suppresses the lethality seen when pblΔN-term.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU. Adult flies exhibit a strong eye phenotype.

      One copy of each of Rac1J10, Rac2Δ and MtlΔ fails to suppress the lethality seen when pblΔN-term.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

      (van Impel et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4twi.2PE/pblΔN-term.UAS.Tag:HA fails to rescue pbl3

      (van Impel et al., 2009)
      Comments

      Expression of pblΔN-term.Scer\UAS.T:Ivir\HA1 in the mesoderm under the control of Scer\GAL4twi.2PE fails to rescue the mesodermal migration defects seen in pbl3 homozygous mutants.

      (van Impel et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      pblΔN-term.Scer\UAS.T:Ivir\HA1
      pblΔN-term.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)