FlyBase Allele Report: Dmel\Slmap[GD3550]

General Information

Symbol

Dmel\Slmap^GD3550

Species

D. melanogaster

Name

FlyBase ID

FBal0263625

Feature type

allele

Associated gene

Dmel\Slmap

Associated Insertion(s)

Carried in Construct

P{GD3550}

Also Known As

UAS-slmap RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Mummery-Widmer et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Mummery-Widmer et al., 2009)

Mutagen

in vitro construct

(Mummery-Widmer et al., 2009)

Progenitor genotype

Carried in construct

P{GD3550}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Mummery-Widmer et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3550

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^en.PU

(Zheng et al., 2017)

atrophy | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

flightless, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021, Schnorrer et al., 2010)

increased body size | P-stage, with Scer\GAL4^Mef2.PR

(Guo et al., 2023)

lethal | parental effect, with Scer\GAL4^bam.PU

(Mao et al., 2022)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

sterile, with Scer\GAL4^bam.PU

(Mao et al., 2022)

viable, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

indirect flight muscle cell | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

myofibril, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

wing, with Scer\GAL4^en.PU

(Zheng et al., 2017)

Detailed Description

Statement

Reference

The expression of Slmap^GD3550 under the control of Scer\GAL4^Mef2.PR leads to complete indirect flight muscle atrophy during pupal development (after 32h APF); fibers are longer than in controls and fail to compact at 32h APF.

(Kaya-Çopur et al., 2021)

Expressing Slmap^GD3550 under the control of Scer\GAL4^C587 (together with Dicer-2, for a more efficient RNAi) does not induce any obvious L3 spermatogenesis defects.

(La Marca et al., 2019)

Expression of Slmap^GD3550 under the control of Scer\GAL4^en.PU results in reduced size of the posterior compartment of adult wings.

(Zheng et al., 2017)

Expression under the control of Scer\GAL4^Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in degenerate myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in missing sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development