FlyBase Allele Report: Dmel\pnt[HMS01452]

General Information

Symbol

Dmel\pnt^HMS01452

Species

D. melanogaster

Name

FlyBase ID

FBal0263360

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01452}

Also Known As

UAS-pntRNAi, UAS-pnt RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01452}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01452

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of pnt^HMS01452 under the control of Scer\GAL4^pnt-14-94 leads to an increase in the number of type II neuroblasts, and to a decrease in mature intermediate neural progenitors per type II neuroblast lineages in the larval brain.

(Chen et al., 2021)

In air sac primordium tip clones expressing pnt^HMS01452 under the control of Scer\GAL4^btl.B123, the mutant cells extend significantly fewer long oriented cytonemes than control clones.

(Du et al., 2018)

Expression of pnt^HMS01452 under the control of Scer\GAL4^pnt-14-94 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a significant increase in the total number of type II neuroblasts, but a decrease in the number of Asense-negative neuroblasts, and complete elimination of intermediate neural precursors in third instar larval brains, as compared to controls.

Expression of pnt^HMS01452 under the control of Scer\GAL4^GMR9D11 leads to an increased number of type II neuroblasts, although this is dependent on the genomic location of the Scer\GAL4^GMR9D11 allele.

(Xie et al., 2016)

Expression of pnt^HMS01452 under the control of Scer\GAL4^CY2 results in eggshells with a single wide dorsal appendage, instead of the two normally seen in wild type.

(Cheung et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^pnt-14-94, pnt^HMS01452 has increased cell number | third instar larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^pnt-14-94, pnt^HMS01452 has abnormal cell number | larval stage phenotype, suppressible | partially by Six4^HM05254/Scer\GAL4^pnt-14-94

(Chen et al., 2021)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by Six4^HM05254/Scer\GAL4^pnt-14-94

(Chen et al., 2021)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has decreased cell number | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Xie et al., 2016)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Xie et al., 2016)

Enhancer of

Statement

Reference

pnt^HMS01452, Scer\GAL4^Act.PU is an enhancer of neoplasia | larval stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU

(Ito and Igaki, 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^pnt-14-94, pnt^HMS01452 has type II neuroblast | third instar larval stage phenotype, enhanceable by Df(2L)erm²/+

(Xie et al., 2016)

Suppressed by

Statement

Reference

Scer\GAL4^pnt-14-94, pnt^HMS01452 has embryonic/larval brain | larval stage phenotype, suppressible | partially by Six4^HM05254/Scer\GAL4^pnt-14-94

(Chen et al., 2021)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has type II neuroblast | increased number | larval stage phenotype, suppressible | partially by Six4^HM05254/Scer\GAL4^pnt-14-94

(Chen et al., 2021)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has intermediate neuronal progenitor | larval stage | decreased number phenotype, suppressible | partially by Six4^HM05254/Scer\GAL4^pnt-14-94

(Chen et al., 2021)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has intermediate neuronal progenitor | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Xie et al., 2016)

Scer\GAL4^pnt-14-94, pnt^HMS01452 has type II neuroblast | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Xie et al., 2016)

Enhancer of

Statement

Reference

pnt^HMS01452, Scer\GAL4^Act.PU is an enhancer of eye-antennal disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU

(Ito and Igaki, 2021)

Additional Comments

Genetic Interactions

Statement

Reference

pros¹⁷/+ partially suppresses the loss of intermediate neural progenitors and Asense-negative neuroblasts seen in larvae expressing pnt^HMS01452 under the control of Scer\GAL4^pnt-14-94.

Df(2L)erm²/+ significantly enhances the increase in type II neuroblasts seen in larvae expressing pnt^HMS01452 under the control of Scer\GAL4^pnt-14-94.

(Xie et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35038

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01452}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pnt^HMS01452

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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